D-I-TASSER Q15198

D-I-TASSER results for Q15198

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q15198
Protein Name: Platelet-derived growth factor receptor-like protein
Gene Name: PDGFRL

Input Sequence in FASTA format

>Q15198 (375 residues)
MKVWLLLGLLLVHEALEDVTGQHLPKNKRPKEPGENRIKPTNKKVKPKIPKMKDRDSANS
APKTQSIMMQVLDKGRFQKPAATLSLLAGQTVELRCKGSRIGWSYPAYLDTFKDSRLSVK
QNERYGQLTLVNSTSADTGEFSCWVQLCSGYICRKDEAKTGSTYIFFTEKGELFVPSPSY
FDVVYLNPDRQAVVPCRVTVLSAKVTLHREFPAKEIPANGTDIVYDMKRGFVYLQPHSEH
QGVVYCRAEAGGRSQISVKYQLLYVAVPSGPPSTTILASSNKVKSGDDISVLCTVLGEPD
VEVEFTWIFPGQKDERPVTIQDTWRLIHRGLGHTTRISQSVITVEDFETIDAGYYICTAQ
NLQGQTTVATTVEFS

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q15198-D1	1-375	375		MKVWLLLGLLLVHEALEDVTGQHLPKNKRPKEPGENRIKPTNKKVKPKIPKMKDRDSANSAPKTQSIMMQVLDKGRFQKPAATLSLLAGQTVELRCKGSRIGWSYPAYLDTFKDSRLSVKQNERYGQLTLVNSTSADTGEFSCWVQLCSGYICRKDEAKTGSTYIFFTEKGELFVPSPSYFDVVYLNPDRQAVVPCRVTVLSAKVTLHREFPAKEIPANGTDIVYDMKRGFVYLQPHSEHQGVVYCRAEAGGRSQISVKYQLLYVAVPSGPPSTTILASSNKVKSGDDISVLCTVLGEPDVEVEFTWIFPGQKDERPVTIQDTWRLIHRGLGHTTRISQSVITVEDFETIDAGYYICTAQNLQGQTTVATTVEFS

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.7323

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	3b43A	0.73	3.58	0.149	0.885	model1_3b43A.pdb.gz
2	5o5gA	0.70	4.15	0.143	0.859	model1_5o5gA.pdb.gz
3	4ofyD	0.61	5.47	0.112	0.864	model1_4ofyD.pdb.gz
4	2wimA	0.59	3.32	0.141	0.701	model1_2wimA.pdb.gz
5	1e07A	0.58	5.27	0.085	0.827	model1_1e07A.pdb.gz
6	5lf5A	0.58	5.43	0.099	0.821	model1_5lf5A.pdb.gz
7	2wngA	0.57	4.02	0.141	0.717	model1_2wngA.pdb.gz
8	6iaaA	0.57	5.03	0.153	0.773	model1_6iaaA.pdb.gz
9	5sznA	0.56	5.28	0.057	0.787	model1_5sznA.pdb.gz
10	5dzvA	0.55	5.37	0.076	0.779	model1_5dzvA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.64	catalytic activity
GO:0016740	0.59	transferase activity
GO:0016773	0.58	phosphotransferase activity, alcohol group as acceptor
GO:0016301	0.58	kinase activity
GO:0004672	0.57	protein kinase activity
GO:0004713	0.56	protein tyrosine kinase activity
GO:0004888	0.50	transmembrane signaling receptor activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.95	cellular process
GO:0044699	0.94	single-organism process
GO:0044763	0.89	single-organism cellular process
GO:0032502	0.84	developmental process
GO:0044767	0.83	single-organism developmental process
GO:0065007	0.80	biological regulation
GO:0050789	0.79	regulation of biological process
GO:0050794	0.76	regulation of cellular process
GO:0048856	0.74	anatomical structure development
GO:0048869	0.72	cellular developmental process
GO:0008152	0.68	metabolic process
GO:0071704	0.66	organic substance metabolic process
GO:0044238	0.65	primary metabolic process
GO:0044237	0.65	cellular metabolic process
GO:0048468	0.62	cell development
GO:0030154	0.59	cell differentiation
GO:0016043	0.59	cellular component organization
GO:0044702	0.58	single organism reproductive process
GO:0006928	0.56	movement of cell or subcellular component
GO:0003006	0.56	developmental process involved in reproduction
GO:0051239	0.55	regulation of multicellular organismal process
GO:0048518	0.55	positive regulation of biological process
GO:0006807	0.55	nitrogen compound metabolic process
GO:0022412	0.54	cellular process involved in reproduction in multicellular organism
GO:0050793	0.53	regulation of developmental process
GO:0048522	0.53	positive regulation of cellular process
GO:0032501	0.53	multicellular organismal process
GO:0007281	0.53	germ cell development
GO:0007165	0.53	signal transduction
GO:0050896	0.52	response to stimulus
GO:0048515	0.52	spermatid differentiation
GO:0019222	0.51	regulation of metabolic process
GO:2000026	0.50	regulation of multicellular organismal development
GO:0080090	0.50	regulation of primary metabolic process
GO:0060255	0.50	regulation of macromolecule metabolic process
GO:0048583	0.50	regulation of response to stimulus
GO:0031323	0.50	regulation of cellular metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0044425	0.94	membrane part
GO:0016020	0.94	membrane
GO:0031224	0.93	intrinsic component of membrane
GO:0044424	0.90	intracellular part
GO:0016021	0.88	integral component of membrane
GO:0005886	0.75	plasma membrane
GO:0043226	0.74	organelle
GO:0044459	0.70	plasma membrane part
GO:0043227	0.70	membrane-bounded organelle
GO:0043229	0.67	intracellular organelle
GO:0043231	0.63	intracellular membrane-bounded organelle
GO:0031226	0.60	intrinsic component of plasma membrane
GO:0044444	0.59	cytoplasmic part
GO:0005887	0.58	integral component of plasma membrane

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2pffB	0.246	7.19	0.057	0.429	2.3.1.86	297
2	0.060	3ejjX	0.373	3.69	0.084	0.459	2.7.10.1	NA
3	0.060	1wd8A	0.380	6.58	0.052	0.619	3.5.3.15	NA
4	0.060	1g0dA	0.366	6.40	0.032	0.560	2.3.2.13	NA
5	0.060	2nziA	0.472	5.60	0.072	0.704	2.7.11.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	2j8o0	0.389	3.84	0.144	0.483	0.55	III	complex1.pdb.gz	82,86,87,90,91,95,125,127,249,252,253,254,255,257
2	0.01	1ry71	0.380	4.39	0.134	0.499	0.56	III	complex2.pdb.gz	83,84,85,86,87,138,168,170,175,196,197,230,231
3	0.01	2qvfB	0.345	4.86	0.089	0.464	0.57	CA	complex3.pdb.gz	300,302,365
4	0.01	2b1hL	0.281	5.60	0.059	0.413	0.60	III	complex4.pdb.gz	144,146,150,151

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.