D-I-TASSER Q14749

D-I-TASSER results for Q14749

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q14749
Protein Name: Glycine N-methyltransferase
Gene Name: GNMT

Input Sequence in FASTA format

>Q14749 (295 residues)
MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQR
VLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMT
LDKDVPQSAEGGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNY
DHILSTGCAPPGKNIYYKSDLTKDVTTSVLIVNNKAHMVTLDYTVQVPGAGQDGSPGLSK
FRLSYYPHCLASFTELLQAAFGGKCQHSVLGDFKPYKPGQTYIPCYFIHVLKRTD

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q14749-D1	1-38,186-247	100		MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTTGCAPPGKNIYYKSDLTKDVTTSVLIVNNKAHMVTLDYTVQVPGAGQDGSPGLSKFRLSYYP
2	Q14749-D2	39-185,248-295	195		RSRTAEYKAWLLGLLRQHGCQRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLDKDVPQSAEGGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNYDHILSHCLASFTELLQAAFGGKCQHSVLGDFKPYKPGQTYIPCYFIHVLKRTD

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.78

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1d2gA	0.73	3.45	0.706	0.837	model1_1d2gA.pdb.gz
2	1wznA	0.64	3.80	0.171	0.776	model1_1wznA.pdb.gz
3	3d2lC	0.64	3.30	0.175	0.749	model1_3d2lC.pdb.gz
4	1y8cA	0.64	3.48	0.117	0.763	model1_1y8cA.pdb.gz
5	5gwxA	0.63	3.70	0.267	0.752	model1_5gwxA.pdb.gz
6	2vdwA	0.63	3.94	0.086	0.773	model1_2vdwA.pdb.gz
7	3bxoA	0.60	3.89	0.189	0.736	model1_3bxoA.pdb.gz
8	2iipA	0.59	3.71	0.122	0.715	model1_2iipA.pdb.gz
9	2avnA	0.58	3.57	0.217	0.695	model1_2avnA.pdb.gz
10	1ve3B	0.57	3.90	0.159	0.702	model1_1ve3B.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.93	catalytic activity
GO:0016741	0.92	transferase activity, transferring one-carbon groups
GO:0008168	0.91	methyltransferase activity
GO:0008757	0.90	S-adenosylmethionine-dependent methyltransferase activity
GO:0043167	0.55	ion binding
GO:0008170	0.53	N-methyltransferase activity
GO:0043168	0.50	anion binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0008152	1.00	metabolic process
GO:0009987	0.80	cellular process
GO:0044237	0.77	cellular metabolic process
GO:0044699	0.68	single-organism process
GO:0071704	0.66	organic substance metabolic process
GO:0044710	0.62	single-organism metabolic process
GO:0044281	0.60	small molecule metabolic process
GO:0044238	0.59	primary metabolic process
GO:0044763	0.57	single-organism cellular process
GO:0006732	0.53	coenzyme metabolic process
GO:0006807	0.52	nitrogen compound metabolic process
GO:0006790	0.51	sulfur compound metabolic process
GO:0046500	0.50	S-adenosylmethionine metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0005737	0.86	cytoplasm
GO:0044444	0.64	cytoplasmic part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1l1eA	0.535	3.97	0.108	0.654	2.1.1.79	NA
2	0.060	1wznA	0.645	3.80	0.171	0.776	2.1.1.-	NA
3	0.060	2i62C	0.579	3.69	0.133	0.712	2.1.1.1	22,67,89,92,96,144,195
4	0.060	2h11B	0.553	3.59	0.124	0.664	2.1.1.67	78
5	0.060	1xxlA	0.521	3.41	0.131	0.614	2.1.1.-	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.79	1d2hB	0.704	2.76	0.745	0.776	1.73	SAH	complex1.pdb.gz	65,70,86,88,91,117,118,142,145
2	0.22	2avnA	0.584	3.71	0.218	0.705	0.87	SAI	complex2.pdb.gz	65,66,67,86,87,88,91,117,118,119,139,140,142,145,146
3	0.12	3bgvD	0.589	3.88	0.118	0.725	0.87	SAH	complex3.pdb.gz	65,66,67,86,87,116,139,140,141,144,159
4	0.11	1d2g0	0.731	3.45	0.706	0.837	1.18	III	complex4.pdb.gz	67,86,87,88,89,90,91,93,97,99,100,103,104,109,115,117,118,142,145
5	0.11	1xvaA	0.733	3.48	0.706	0.841	1.09	SAM	complex5.pdb.gz	65,70,71,139,142,145,178

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.