D-I-TASSER Q14240

D-I-TASSER results for Q14240

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q14240
Protein Name: Eukaryotic initiation factor 4A-II
Gene Name: EIF4A2

Input Sequence in FASTA format

>Q14240 (407 residues)
MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQ
QRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKV
ILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMF
VLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKE
ELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTV
SALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHR
IGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q14240-D1	1-243	243		MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELT
2	Q14240-D2	244-407	164		LEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.89

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	2hyiI	0.87	2.18	0.677	0.934	model1_2hyiI.pdb.gz
2	4d25A	0.87	2.37	0.293	0.939	model1_4d25A.pdb.gz
3	2db3A	0.86	2.52	0.281	0.939	model1_2db3A.pdb.gz
4	3i61A	0.85	2.84	0.280	0.946	model1_3i61A.pdb.gz
5	3pewA	0.84	2.44	0.404	0.911	model1_3pewA.pdb.gz
6	5supA	0.83	2.37	0.366	0.907	model1_5supA.pdb.gz
7	5v9xA	0.80	2.71	0.185	0.889	model1_5v9xA.pdb.gz
8	6vz4K	0.76	3.90	0.111	0.909	model1_6vz4K.pdb.gz
9	6tdaS	0.76	3.77	0.118	0.902	model1_6tdaS.pdb.gz
10	6kw5Q	0.75	3.83	0.119	0.894	model1_6kw5Q.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0016787	1.00	hydrolase activity
GO:0008026	0.99	ATP-dependent helicase activity
GO:0004004	0.94	ATP-dependent RNA helicase activity
GO:1901363	0.60	heterocyclic compound binding
GO:0097159	0.60	organic cyclic compound binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0044260	0.99	cellular macromolecule metabolic process
GO:0044238	0.99	primary metabolic process
GO:0034641	0.99	cellular nitrogen compound metabolic process
GO:0090304	0.98	nucleic acid metabolic process
GO:0016070	0.94	RNA metabolic process
GO:0010501	0.92	RNA secondary structure unwinding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0032991	0.76	macromolecular complex
GO:0043229	0.74	intracellular organelle
GO:0030529	0.70	intracellular ribonucleoprotein complex
GO:0044446	0.66	intracellular organelle part
GO:0044444	0.66	cytoplasmic part
GO:0044428	0.55	nuclear part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.350	3i62A	0.852	2.84	0.280	0.946	3.6.4.13	61,81,84,336,338,366,368
2	0.293	1xtkA	0.567	5.54	0.249	0.808	3.6.4.13	184
3	0.140	2hyiI	0.871	2.18	0.677	0.934	3.6.4.13	51,53,57,59,78,81,84,116,246,336,338,366,368
4	0.103	3iuyA	0.491	1.82	0.297	0.513	3.6.4.13	NA
5	0.100	3eiqD	0.624	4.38	0.641	0.774	3.6.4.13	59,79,81,84,110,113,184,187,191,215

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.85	2hyiC	0.881	2.25	0.690	0.946	1.45	ANP	complex1.pdb.gz	53,55,60,79,80,81,82,83,84,85,184,336,338,363,366
2	0.67	3ex7C	0.872	2.17	0.677	0.934	1.10	AF3	complex2.pdb.gz	79,80,83,184,215,336
3	0.11	2j0s5	0.872	2.15	0.675	0.934	1.27	III	complex3.pdb.gz	101,170,173,174,175,197,198,201,202,203,204,205,206,226,227,228,256,257,258,282,284,285,288,349,351,378,381,383,384
4	0.11	2hyiC	0.881	2.25	0.690	0.946	0.90	RQA	complex4.pdb.gz	109,110,111,137,159,161,162,193,196,312,330,331,332
5	0.11	2j0s3	0.872	2.15	0.675	0.934	1.06	III	complex5.pdb.gz	58,62,81,243,251,353,354,355,367,368,386,387,390,391,393,394,396,397

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.