D-I-TASSER results for Q13464-D1

Input Sequence in FASTA format

>Q13464 (1354 residues)
MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYK
DTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAF
FWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTA
EVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDY
ISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFP
DDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLS
SDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNA
DKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLEST
VSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKL
SQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTD
KDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLN
HSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKL
KEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVKNLTLQLEQES
NKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMR
ELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAE
SEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENE
ELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRK
KANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQL
ASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKK
QYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILY
ANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALE
CRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNP
PSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS

Predicted Secondary Structure

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820                 840                 860                 880                 900                 920                 940                 960                 980                1000                1020                1040                1060                1080                1100                1120                1140                1160                1180                1200                1220                1240                1260                1280                1300                1320                1340
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Sequence
MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS
Prediction
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCSSSSSSSSCCCCSSSSSSSSCCCCSSSSSSSCHHHHHHHHHHHHHHHHHHHHHHCCCCCSCCSSSSSSCCCSSSSSSCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCSSCCCCHHHSSSCCCCCSSSSCCCCCCCCCCCCCCCSCCCCCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCSSSSSSSSCCCCCCCCCCCSSSSSSSSCCSSSSSSCCCCCCCCCCSSSSCCCCCCCSSCCCHHHHHHCCCCCCCHHHHSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSSSCCCCCCCCCCCCCCCCCCCCCCCSSSCCCCCSSCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Confidence
9988627899985234315898777645666688875310476132033428999998524554541148986664367787516770799999937995899987329999878778999999999985799827123456516993899971457549999998559984999999999999999999729571147834502568997998144112125889982023445683444589984489988888336589689999998538999999999999999981777789999765599999999998258312489986999971966799997899843899997664599762011688766788767899986566545677996232771010357877565553100000222112578999987666666678999988762438999999987666789999999999999999999999999987424688877899999888775889999998889999999999999999999999999999999999999999986999999999999999999999999999999999999999999999999999999999999999999999999999999999999999998788999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999986528999999999999999999999998889999999999999999999999999999999999999999999999999999999999999999999999999862033127899999999856666543111256776415555654344658889763023320032244456555268999985178766542678878889887776512136789988654234666789997344168888731468998307998467885268999967769994087544569813687178664231154565321670126612210114777787876555555555433224577225666448998530136510145579986141246731142101688655678877777114666554458978999999999863367899986678998778888876555677788899999

H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820                 840                 860                 880                 900                 920                 940                 960                 980                1000                1020                1040                1060                1080                1100                1120                1140                1160                1180                1200                1220                1240                1260                1280                1300                1320                1340
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Sequence
MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS
Prediction
5444442441144046314445341224000100300143043452455541440254246435524644031510300100020010001002225343000000021540154521420330240033051000010100010441000000010000000103546052630000000000001101532000000100000013400000000000022346442302000001000000002333454511100000000000000000000012523440142014344504124455016402300330043144101440141014130035010420343614341204154650121026344655454344345424444430320000102120233344444344443443454355424434541454454455145445634440443254145455445414441441454254145404404542544454444344414424542543444454344245414413431440354145445435414544542454354255135404534541463454245434414541453454455446424414542540454055145415434543542454244345435534451454044135414534563444454045434534644444244245425404544443444444345425404441541454255145445404541550454344444444424540541444354044425414511540454454145414404542541442044045414434414531452154045405644553444254045225404541544454344444434414442441454345334414541453454254045435414540551454355045414534542544664435524541454144544344444444454464564646655444641444456455344525434642454144244415514443444453144214214344240440443144244425444444454464444431010001003465433430420000004210000124644543301010303441114313442123245520320030123444446444444444554444444232230022303320303203400000032110010440413003412445520011031224242022000004246314300320143044454444444223432444444422344444466568

Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank	PDB hit	ID1	ID2	Cov	Norm. Zscore	Download alignment		20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820                 840                 860                 880                 900                 920                 940                 960                 980                1000                1020                1040                1060                1080                1100                1120                1140                1160                1180                1200                1220                1240                1260                1280                1300                1320                1340                    |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |
							SS	CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCSSSSSSSSCCCCSSSSSSSSCCCCSSSSSSSCHHHHHHHHHHHHHHHHHHHHHHCCCCCSCCSSSSSSCCCSSSSSSCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCSSCCCCHHHSSSCCCCCSSSSCCCCCCCCCCCCCCCSCCCCCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCSSSSSSSSCCCCCCCCCCCSSSSSSSSCCSSSSSSCCCCCCCCCCSSSSCCCCCCCSSCCCHHHHHHCCCCCCCHHHHSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSSSCCCCCCCCCCCCCCCCCCCCCCCSSSCCCCCSSCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
							Seq	MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS
1	4iw0A	0.15	0.07	2.32	1.02	CEthreader		------------------------------------------------------------------GSQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQM--REFEVLKKLNHKNIVKLFEEETTTRHKVLIMEFCPCGSLYTVLEAYGLPESEFLIVLRDVVGGMNHLRENGIVHRNIKPGNIMRDGQSVYKLTDFGAARELEDDEQ---FVLYGTEEYLHPDMYERAVLRKDYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIF-HELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRY-DLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQET-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2	4oauC	0.07	0.03	1.10	0.85	EigenThreader		--------------------------------------DNHLLIKAVQNEDVDLVQQLLEGG--------------------ANVNFQEEE-----GGWTPLHNAVQMSLLRHGADPVLRKATPFILAAIAGSVKLLKLFLSKGADVN----ECDFYGFTAFMEAAVYGKVKALKFLYKR----------GANVNLRRKTKEDQERLRKGGATALMDAAEKGHLKILLDEMGADVNACDNMGRNALIHALLSSDDSDVEAITHLLLDHGADVNVRGERGKTPLILAVEKLEQEHIEINDTDSDGKTALLLAVELIAELLCKRGASTDCGDLVMTARRNYDHSLVKVLLSHGAKEDFEDWKPQSSHWGAALKDLHRIYRPMIGKLKFFIDEKYEIYLGFYEKQEVAVKTFCEGSPRAQREVSCLQSEACLDVHRGEDFARNVLSSIFKAVQELHLSCGYTHRDLEDLGRLVLYVVKKGSISFEDLKVVQLETKDLIHRLFHLLGHESRYRTLRNVGNESDIKTILRLLTTKIVMKKMNKFYEGDLLKFIRNLGENIDEEMKLKIGDPSLYFQKTFPD------LVIYVYTKLQNTEYRKHFPQT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
3	6yvuB	0.14	0.09	3.23	2.48	FFAS-3D		-------------------------------------------------------------------------------------------------------------------------------------------FKSYAGHTSFSAVV--GPNGS-----------------GKSNVIDSMLFV--FGFRANKMRQDRLLIHKSEAFPSLQSCSVVIDESSGTSRIDEEKPG---LIITRKAFKNNSSKYEKESSYTEVTKLLKNEGIDLDHKRFLILQGEVENIAQMKPKAEKESDDGLLEYLEDII-----------GTANYKPLIEER-----MGQIENLNEVCLEK-ENRFEIVD-REKNSLESGKETALEF--------------------------------LEKEKQLTLLRSKLFQFKLLQSNSKLASTLEKISSSNKDLEDEKMKFQESLKKVDEIKAQRKEIKDRISSCSSKEKTLVLERRELEGTRVSLEERTKNLVSKMEKAEKTLKSTKHSISEAE---NMLEELRGQQTEHETEIK---DLTQLLEKERSILDDIKLSLKDKTKNISAEIIRHEKELEPWDLQLQEKESQIQLAESELSLLEETQAKLKKNVETLEEKILAKKTHKQELQDLILDLKKKLNSLKDERS----QGEKNFTSAHLKLKEMQKVLNAHRQRAMEARSSLSKAQNKSKVLTALSRLQKSDTVECAQHCIDYLRKNFDLVKPKNPKFSNAFYSVLRDDDYTPEEVDKIERELSERENNFRVASDTVHEMEEELKKLRDHEPDLESQISKAEMEADSLASELTLAEQQVKEAEMAYVKAVSQLNVVMKNLERLRGEYNDLQSETKTKKQMQNSKVESVCQKLDILVAKLKKVKSASKKSGGDVVKFQKLLQNSEKVIEEQLKHTKLALAENDTNMNETLNLKVELKEQSEQLKEQMEDMEESINEFKSIEI-EMKNKLEKLNSLLTYLDVELIESKINELSYYVEETNVDIGVLEEYARRLAEFKRRKLDLNNAVQKRDEVKEQLGILKKKRFDEFMAGFNIISMTLKEMYQMITNLSGGEKTLSSL-ALVFAL---HKYKPTPLMDEIDAALDFRNVSIVANY-----IKERTKNAQFIVISLRNNMFELAQQLVKRDNRTKSTTIKNI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
4	6r9tA	0.11	0.10	3.78	1.69	SPARKS-K		HSQVDAITAGTASVVNLTAGDPAETDYTAVGCAVTTISSNLLLAALLEDGSGRPLLQAAKGLAGAVSELLRSAQPLQAAGNVGQASGELLQQIGESDTDPHFQDALMQLAKAVASAAAALVLKATQCALSTSQLVACVVAPTISSPV----CQEQLVEEGCVSASQAATQLLRGVGAAATAVTQALNELLQHVKAHAT---GAGPAGRYDQA----TDTILTVTE----NIFSSMGDAGEAIKADAEGESDLENATNAAAQNAIKKKLLEHAAKQAAASATQTIAAAQHAASTPPQPLLVQSKAVAEQIPLLVQGVRGSSFLQPGGKMVAAAKASVPTIQDQASAMQLSQCAKNLGTALAQEACGPLEMDLSVVQNLEKDLQEVKAAARDG----------------KLKPLPGETMEKCTQDLGNSTKAVSSAIAQLLGEVAQYAGIAARDVAGGLRSLAQAARGVAALTSDPAVQAIVLDTASDVLDKASSLIEEAKKAAGHDPESQQRLAQVAKAVTQALNRCVSVDNALRAVGDASKRLLGLNQAATELVQASRGTPQDLARASGRFGQDFSTFLEAGVEMEDRAQVVSNLKSTDPAAPNLKSQLAAAARAVTDPGQKECDNALRELETVRELYFGCLDSVMENSKVLGEAMTGISQNAKNGNLPEFAISTASKALCGFTEAAAQAAYLVGVSDPNSQAGFARANQAIQMACQSLGAQVLSAATIVAKHTSALCNSCRLASARKRQFVQSAKEVANSTANLVKTIKALRAQCRAATAPLLEAVDNLSAFASNPEFSPEGRAAMEPIVISAKTMLESAGGLIQTARALAVNPR-DPPSWSVLAGHSRTVSDSIKKLITSMRETAIAALNSCLRDLDQASLAAVSLTAVQEISHLIEPLANAARAHKVSQMAQYFEPLTLAAVGAASKTLSHPQQMALLDQTKTLAESALQLLYTAKEATQEALEEAVQMMTEAVEDLTTTLNEAASAA-GVVGGMVDSITQAINQLDE-----GPMGEPEG-SFVDYQTTMVRTAKAIAVTVQEMVTKSNTSPEELGPLANQLTSDYGRLASEAAVAAENEEIGSHIKHRVQELGHGCALQCSPSDAYTKKELIECKVSHVLAAGNRGTQ-------------------ACITAASAVSGIIADLDTTIMFGTLNREGTETFADHREGILKTAKVLVEDTKVLVQNASQEKLAQAAQSSVATTRLADVVKLGAASLG-AEDPETQVVLINAVKDVAKALGDLISATKAAAGKVGD-----------DPAVWQLKNSAKMVTNVTSLLKTVKAVEDEATKGTIRQELAVFCSPEPPAKTSTPEDFIRMTKGI
5	4d1eA	0.11	0.06	2.33	1.40	CNFpred		------CNSHLRKAGTQIENIEEDFRNLMLMLLLEVISG-ERLPKPDRGKMRFHMIANVNMALDYIASKGVKLVSIGAEEIVDG--NVKMTLG------MIWTIILRFAIQDISAKEGLLLWCQRMTAPYR-NVNIQNFHTSWMDGLGLCALIHRHR--DLIDYSKLN--------DPIGNINLAMEIAEK------------------------------------------HLDIPKMLDAEDIVNT--------MPDERAIMTYVSCFYHAFGAEQAETAANRICKVL------------AVNQENERLMEEYER---------LASELLEWIRR-----TIPWLENRTPAAT--------------------------------------------------------------------MQAMQKMLEDFRDYRRMHMPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSE-GKMVSDIAGAWQRLEQAEKGYEE-----------------WLLNEIRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKD-----ESASLTEVRALLRMHEAFESDLAAHQDRVEQI-------AAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERME----------------------KLLETIDQLHLEFAKRAAPF------------------NNWMEGAMEDLQDMF--IVHSIEEIQSLITAHEQFMATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTV-------------TMDELRTKWDKVKQLVPIRDQSLQEELARQHA-------NERLRRQFAAQANAIGPWIQNKMEEIARSSIQITG----ALEDQMNQLKQYEHNIINYMNNIDKLEGDHQLIQEALVFDNKHTNY--------------TMEHIRVGWELLLTTIARTINEVETQILTRDAKGI-----TQEQMNEFRASFNHDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTV---------TFQSFIDFMTR-TDTAEQVIASFRKPYILAEELRREL--PPDQAQYCIKRMPAYS----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
6	1vw1A	0.05	0.02	0.89	0.50	DEthreader		--------------------------------------------------------------------------------------------------------SQCGFNCLTD-------I---------------------------------------------------------------------------------------------------------------------------------------------------------F-------------------------------------------------------------------------------H--L----------SWSETHDLYHDAQQAQKDNRLYEARIL-RA-P--Q------------LAPNAELIGYNNFSGRASQYVAPGTVSSFSPALSQQNMDIELSTLSLSNELLLESILSGAYKRYYNLSDEELSQKASQEYSNN-YQMDVEYKFKAYLSIKLNDKREFE--TGDEE----NKDGKIKNKNLNLGLDLEGKTFTSTSYNKTPGDGA----------TKYTSEAVETQEHIVQYCQALAQLEMVYEAFRLFVTKPEMFFADWVANILQWVNVAQQLNVAGVLTAGLNSAN-H----------TRIAEAIASIQLYVNRALENVENSSI-------------------------------------------------QKEITKETDYRYELK-AHWNTPITFDVNKKIPGMFYMGQSNVYRDNSYQED-AASSD----------------------NALEIDGLSIDVTFYESFSIP-VT-LKVSTDNALTLH----------------------------QYMQWQSY----------------------LGKGFYAT-FVIPPYNLST--WFKLYSGQLTD--INI-TL--FIPLDDVPLNQDYHAK--------PSDGTWWGPHFLTHFES---EPMDFSGANSLYFWELFYYT--L---R--------VW---RPLLEDTSWNSDPLDSVDP--V--H-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HDSAIVALRQNIPTRSA--L-------------WQT-AQVYRHLPYPI
7	7kogB	0.08	0.06	2.51	1.79	MapAlign		------EEMRKLEELVATTQAALEKEEKARKEVEALNAK------LIQEKTDLLRNLEGEKGSISSIQEKAAKLQ-------AQKSDLESQLMDTQERLQQEEDNRNQMFQQKKKLEQEVGGLKKDIEDLELSLQKSDQDKAS-------------KDHQIRNLNDEIAHQDELINKLNKEKKMQGEHTQK--------------------------------TAEELQASEDKVNHLTKVKAKLEQTLDELEDSLEREKKLRGDVEKAKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEIASLTAKLEDEQSIVSKTQKQIKELQSRIEELEEEVEAERQARGKAE----------------------------------------------------------------KQRADLARELEELGERLEEAGGATSAQIELNKKREAEMSKLRRDLEESNIQHESTLAN---LRKKHNDAVSEMGEQIDQLNKLKTKAEHDRTHVQNDLNNTRHALDQMCREKAATEKIAKQLQHQVNEIQGKLDEANRTLNDFDSAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEARERATLLGKFRNLEHDLDNIREQLEEEAEGKADIQRQLSKANAEAQLWRTKYESEGVAR----AEELEEAKRKLQARLAEAEETIESLNQKVIALEKTKQRLATEVEDLQLEVDRATAIANAAEKKAKAIDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEAQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKQKKRLEVEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANK------------ANSEAQKTIKKYQQQLKDVQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELGDAHEQINELAAQATSASAAKRKLEGELQTLHADLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALETQIKEL------------------------------------------------------------------------------------QIRLDEAETNALKGGKKAIAKL-------------------------------------------------EQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQADEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAIAKF
8	2f2uA	0.85	0.24	6.85	1.12	MUSTER		---------Q-RKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEV-----ETFPIPKAFVGNQLPFIGFTYYR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
9	2f2uA	0.85	0.24	6.81	1.30	HHsearch		----------QRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEVET-----FPIPKAFVGNQLPFIGFTYYR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
10	6cq0A	0.15	0.07	2.21	0.75	CEthreader		------------------------------------------------------------------AMQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFV------------QMREFEVLKKLNHKNIVKLFAIEEETRHKVLIMEFCPCGSLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGNIMRVIGED------------GQSVYKLTDFGAGTEEYLHPDMYE------------DLWSIGVTFYHAATGSLPFRP-----------------------------------------FEGPRRNKEVMYKIITGK------------------------PSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQIHKFDKQKLYYHATKA-MTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQET------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------


(a) ID1 is the number of template residues identical to query divided by number of aligned residues.
(b) ID2 is the number of template residues identical to query divided by query sequence length.
(c) Cov is equal the number of aligned template residues divided by query sequence length.
(d) Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e) Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f) Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Rank^a	Estimated TM-score^b
1	0.4484


(a) D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.
(b) The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)


Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.
1	6r9tA	0.93	2.45	0.096	0.965
2	6jxaA	0.32	10.08	0.038	0.504
3	3jb9A	0.32	9.07	0.036	0.465
4	6yejA	0.32	9.78	0.047	0.485
5	6vr4A	0.31	10.15	0.033	0.498
6	7dxjA	0.31	9.29	0.041	0.456
7	6mu1A	0.31	10.16	0.040	0.486
8	6z2wE	0.30	9.42	0.046	0.448
9	3javA	0.30	9.78	0.030	0.465
10	5x6oC	0.30	9.50	0.043	0.451



(a) Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b) Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c) RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d) IDEN^a is the percentage sequence identity in the structurally aligned region.
(e) Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)


GO term	Cscore^GO	Name
GO:0016301	0.97	kinase activity
GO:0004672	0.96	protein kinase activity
GO:0004674	0.88	protein serine/threonine kinase activity






(a) Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

Biological Process (BP)


GO term	Cscore^GO	Name
GO:0065007	0.90	biological regulation
GO:0050789	0.89	regulation of biological process
GO:0009987	0.89	cellular process
GO:0050794	0.86	regulation of cellular process
GO:0008152	0.77	metabolic process
GO:0071704	0.76	organic substance metabolic process
GO:0044237	0.76	cellular metabolic process
GO:0044260	0.75	cellular macromolecule metabolic process
GO:0044238	0.74	primary metabolic process
GO:0044267	0.73	cellular protein metabolic process
GO:0006468	0.72	protein phosphorylation
GO:0007165	0.68	signal transduction
GO:0035556	0.53	intracellular signal transduction
GO:0044699	0.52	single-organism process






(a) Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

Cellular Component (CC)


GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0044424	0.88	intracellular part
GO:0043226	0.55	organelle
GO:0005737	0.55	cytoplasm
GO:0043229	0.53	intracellular organelle






(a) Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB


Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	3ffzA	0.234	9.63	0.042	0.354	3.4.24.69	NA
2	0.060	1ynnJ	0.083	6.02	0.036	0.100	2.7.7.6	71
3	0.060	1ea0A	0.275	10.66	0.032	0.455	1.4.1.13	NA
4	0.060	2zxqA	0.247	9.31	0.034	0.367	3.2.1.97	NA
5	0.060	2dqmA	0.240	8.70	0.035	0.346	3.4.11.2	NA



(a) Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b) TM-score is a measure of global structural similarity between query and template protein.
(c) RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d) IDEN^a is the percentage sequence identity in the structurally aligned region.
(e) Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

No Binding sites was predicted

References:
1. Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.