D-I-TASSER Q08495-D1

D-I-TASSER results for Q08495-D1

[Click result.zip to download all results on this page]

Input Sequence in FASTA format

>Q08495 (405 residues)
MERLQKQPLTSPGSVSPSRDSSVPGSPSSIVAKMDNQVLGYKDLAAIPKDKAILDIERPD
LMIYEPHFTYSLLEHVELPRSRERSLSPKSTSPPPSPEVWADSRSPGIISQASAPRTTGT
PRTSLPHFHHPETSRPDSNIYKKPPIYKQRESVGGSPQTKHLIEDLIIESSKFPAAQPPD
PNQPAKIETDYWPCPPSLAVVETEWRKRKASRRGAEEEEEEEDDDSGEEMKALRERQREE
LSKVTSNLGKMILKEEMEKSLPIRRKTRSLPDRTPFHTSLHQGTSKSSSLPAYGRTTLSR
LQSTEFSPSGSETGSPGLQNGEGQRGRMDRGNSLPCVLEQKIYPYEMLVVTNKGRTKLPP
GVDRMRLERHLSAEDFSRVFAMSPEEFGKLALWKRNELKKKASLF

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MERLQKQPLTSPGSVSPSRDSSVPGSPSSIVAKMDNQVLGYKDLAAIPKDKAILDIERPDLMIYEPHFTYSLLEHVELPRSRERSLSPKSTSPPPSPEVWADSRSPGIISQASAPRTTGTPRTSLPHFHHPETSRPDSNIYKKPPIYKQRESVGGSPQTKHLIEDLIIESSKFPAAQPPDPNQPAKIETDYWPCPPSLAVVETEWRKRKASRRGAEEEEEEEDDDSGEEMKALRERQREELSKVTSNLGKMILKEEMEKSLPIRRKTRSLPDRTPFHTSLHQGTSKSSSLPAYGRTTLSRLQSTEFSPSGSETGSPGLQNGEGQRGRMDRGNSLPCVLEQKIYPYEMLVVTNKGRTKLPPGVDRMRLERHLSAEDFSRVFAMSPEEFGKLALWKRNELKKKASLF
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHCCC
Confidence	974457889999862589999989997666557788656776667787776556789997555688888887778777777888889877888889887778899987888988889989999999888888888888778899988888888889888887766666678899889999999887677777889876678766677776678888877555655443321000134211356787766555555667888888665566666766777777777778887666677666666777766665555555677776555555444344556777799997421457678998777256553189999999989899999973088899999874789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MERLQKQPLTSPGSVSPSRDSSVPGSPSSIVAKMDNQVLGYKDLAAIPKDKAILDIERPDLMIYEPHFTYSLLEHVELPRSRERSLSPKSTSPPPSPEVWADSRSPGIISQASAPRTTGTPRTSLPHFHHPETSRPDSNIYKKPPIYKQRESVGGSPQTKHLIEDLIIESSKFPAAQPPDPNQPAKIETDYWPCPPSLAVVETEWRKRKASRRGAEEEEEEEDDDSGEEMKALRERQREELSKVTSNLGKMILKEEMEKSLPIRRKTRSLPDRTPFHTSLHQGTSKSSSLPAYGRTTLSRLQSTEFSPSGSETGSPGLQNGEGQRGRMDRGNSLPCVLEQKIYPYEMLVVTNKGRTKLPPGVDRMRLERHLSAEDFSRVFAMSPEEFGKLALWKRNELKKKASLF
Prediction	755546545345513232433414233442234265412526413314534421326222313231223333544453454554434244344444453345343433344443434443144434222315455554534542443444444444444442456423635334434415254324344421432443444566344443445445444544544455435434534455144244424442345435654444453442443442443444443444422434443244233343243344333333444544443434344244445432011420233355555116402453254102571035007133620550251225512464717
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

Hydrogen bond networks

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank

PDB
hit

ID1

ID2

Cov

Norm.
Zscore

Download
alignment

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

SS
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHCCC
MERLQKQPLTSPGSVSPSRDSSVPGSPSSIVAKMDNQVLGYKDLAAIPKDKAILDIERPDLMIYEPHFTYSLLEHVELPRSRERSLSPKSTSPPPSPEVWADSRSPGIISQASAPRTTGTPRTSLPHFHHPETSRPDSNIYKKPPIYKQRESVGGSPQTKHLIEDLIIESSKFPAAQPPDPNQPAKIETDYWPCPPSLAVVETEWRKRKASRRGAEEEEEEEDDDSGEEMKALRERQREELSKVTSNLGKMILKEEMEKSLPIRRKTRSLPDRTPFHTSLHQGTSKSSSLPAYGRTTLSRLQSTEFSPSGSETGSPGLQNGEGQRGRMDRGNSLPCVLEQKIYPYEMLVVTNKGRTKLPPGVDRMRLERHLSAEDFSRVFAMSPEEFGKLALWKRNELKKKASLF

1vt4I3

0.05

2.40

1.05

CEthreader

EDEAIFEEAHKQVQRGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG

5jcss

0.07

2.73

1.31

SPARKS-K

MGCHDSIVKIHLGEQTDAKLLIGTYTSGDKPGTFEWRAGVLATAVKEGRWVLIEDIDKAPTDVLSILLSLLEKRELTIPSRGETV--------KAANGFQLISTVRINEDHQKDSSNKIYNLNMMRIWNVIELEEPSEEDLTHI-LAQKFPILTNLIPKLIDSYKNVKSIYMNTKFISLNKGA--------HTRVVSVRDLIKLCERLDILFKNNGINK----PDQLIQSSVYDSIFSEAADCFAGALEPIIQAIGESLDIASSRISLFLTQHVPTLENLDDSI-------KIGRAVLLKEKLNIQKKSMNSTLFAFTNHSLRLMEQISVCIQMTEPVLLVGETGTGKTTVVQQLAKMLAKKLTVINVSQQTETGDLLGGYKPKTVAVPIQENFETLFNATFSLK

1vt4I

0.06

0.05

2.45

1.87

MapAlign

-----GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGG

1ujsA

0.57

0.12

3.34

3.41

HHsearch

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSSGSSG------------------NAVNWGMREYKIYPYELLLVTTRGRNRLPKDVDRTRLERHLSQEEFYQVFGMTISEFDRLALWKRNELKKQARLF

6ybt1

0.06

2.51

0.90

CEthreader

GGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIILTGHKKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIPARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKNNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMA-----------

4adyA

0.06

2.60

0.78

EigenThreader

WSEISNELPDIEALYDDDTFSDREAALIA--SKVYYNLGEYESAVKYALAAKDRFDIDEKSIEYVQEASKQYTKDEQFYTKDIIDPKLTSIFERIEKCLKASELKLALGIALEGYRLDIIESALKSKLDQSTVTNSKFRSSILRKSFDFLNPNCDYLTLNKVVVNLNDALQLFKKLKEENDEGLSAQIAFDLVSSASQQLLEILVTELTAQGYDPALLNILSGLPTCDYYNTFLLNNKPWLGKAQNWAKFTATASLGVIHKGNLLEGKKVAPYLPGSRASSRFIKGGSLYGLGLIYAGFGHGASLGIGLAAGSANGGLCLGTGKFTYSQETQHGNITRGLAVGLALINYGRSDESLLRYGGAFTIALAYAGTGVIALGFVLLTVPRIVQLLSCGTAFALGIACAG

1ujsA

0.58

0.11

3.19

0.88

FFAS-3D

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGNAVNWGMREYKIYPYELLLVTTRGRNRLPKDVDRTRLERHLSQEEFYQVFGMTISEFDRLALWKRNELKKQARLF

3j3iA

0.11

3.97

1.17

SPARKS-K

RQYLNSNSRLVTQMASKLTGIGLDATPQMRIFSEMDTA-DYADMLHLTIFEGLLVNGEKLLSADRAGYDVLVEELTLANIRIEHHKMPALRLTPRSRTARECDFNPTMNLKALRGSGVKSRRRVSEVFRSPPRRESTTTTDDSPRWLLTRRVPIIDEPPAYESTSSISEGTSQHEEEMGLFDAEELPMQQTVIATEARRRLGRGTLERIQEAALAQGEVTAEKNRRIEAMLSARDPQFTKMLSDGGLGVREREEWLELVDKTVGVKGLKEVRSIDGIRRHLEEYGEREGFAVVRTLLSGNSKHVRRINQLIRESNPSAFETEASRMRRLRADWDGDAGSAPVN--ALHFVGNSPGWK-------RWLENNNIPSDIQVAGKRMCS---YLAEVLSHDATKLGRLV

3hz3A

0.05

0.03

1.41

0.67

DEthreader

------PTTGQPRKNFLLQNGNDW-I-YF-D------KDTGA--GTNAL-K-LQ-FDKGTISADEQYRRGNEPIMQILLMHETWNKQ-L--LANDIDNVVDVDLLLQLTMDDRLSLVNRAND-------------------------TENAVIPSNKDSVAGGQVRFGGIMAHKNQKY--------YNDTDAPVAYTD-NGDLH----------------------------FTNTNLDGQQYTA---------------------TESERVRIAYGSDEDLRNAL-------------ADWVPIGYQKYGGIDPV-I--------FNGTNILHRGAGYVLRS-----------NLGTSTQQFLPSLSVQDNE-GYGFVKHYYDENKQM----------------

1vt4I3

0.06

0.05

2.11

1.68

MapAlign

GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-------------------------------------------------GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG------------GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG--GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG

(a)	ID1 is the number of template residues identical to query divided by number of aligned residues.
(b)	ID2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e)	Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f)	Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.4141

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5j44A	0.35	6.74	0.065	0.575	model1_5j44A.pdb.gz
2	4r04A	0.34	6.59	0.042	0.543	model1_4r04A.pdb.gz
3	7cpxA	0.34	6.44	0.052	0.523	model1_7cpxA.pdb.gz
4	6k7gA	0.33	7.49	0.026	0.578	model1_6k7gA.pdb.gz
5	7kpxC	0.33	7.14	0.055	0.556	model1_7kpxC.pdb.gz
6	5u1sA	0.32	7.29	0.048	0.560	model1_5u1sA.pdb.gz
7	1wxrA	0.32	6.63	0.038	0.523	model1_1wxrA.pdb.gz
8	5hb4B	0.32	6.83	0.033	0.521	model1_5hb4B.pdb.gz
9	6zwmA	0.32	7.02	0.040	0.523	model1_6zwmA.pdb.gz
10	6w2dJ	0.32	7.09	0.024	0.538	model1_6w2dJ.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0008092	0.74	cytoskeletal protein binding
GO:0003779	0.73	actin binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0044699	0.97	single-organism process
GO:0009987	0.97	cellular process
GO:0044763	0.96	single-organism cellular process
GO:0065007	0.87	biological regulation
GO:0050794	0.86	regulation of cellular process
GO:0016043	0.86	cellular component organization
GO:0032502	0.81	developmental process
GO:0022607	0.80	cellular component assembly
GO:0048518	0.79	positive regulation of biological process
GO:0044767	0.79	single-organism developmental process
GO:0048522	0.78	positive regulation of cellular process
GO:1902589	0.75	single-organism organelle organization
GO:0051128	0.73	regulation of cellular component organization
GO:0051130	0.69	positive regulation of cellular component organization
GO:0033043	0.67	regulation of organelle organization
GO:0032879	0.67	regulation of localization
GO:0080090	0.65	regulation of primary metabolic process
GO:0060255	0.65	regulation of macromolecule metabolic process
GO:0031323	0.65	regulation of cellular metabolic process
GO:1903827	0.63	regulation of cellular protein localization
GO:0070201	0.63	regulation of establishment of protein localization
GO:1903533	0.62	regulation of protein targeting
GO:0031344	0.62	regulation of cell projection organization
GO:0050896	0.61	response to stimulus
GO:0051239	0.60	regulation of multicellular organismal process
GO:0050793	0.60	regulation of developmental process
GO:0048519	0.60	negative regulation of biological process
GO:0007165	0.60	signal transduction
GO:0007015	0.60	actin filament organization
GO:1902743	0.59	regulation of lamellipodium organization
GO:0048523	0.59	negative regulation of cellular process
GO:0044087	0.59	regulation of cellular component biogenesis
GO:0065008	0.58	regulation of biological quality
GO:0051240	0.58	positive regulation of multicellular organismal process
GO:0048869	0.58	cellular developmental process
GO:0048583	0.58	regulation of response to stimulus
GO:0042221	0.58	response to chemical
GO:0030029	0.58	actin filament-based process
GO:0022603	0.58	regulation of anatomical structure morphogenesis
GO:0080134	0.57	regulation of response to stress
GO:0051716	0.57	cellular response to stimulus
GO:0051270	0.57	regulation of cellular component movement
GO:0051129	0.57	negative regulation of cellular component organization
GO:0032956	0.57	regulation of actin cytoskeleton organization
GO:0022604	0.57	regulation of cell morphogenesis
GO:0010033	0.57	response to organic substance
GO:0006461	0.57	protein complex assembly
GO:0071495	0.56	cellular response to endogenous stimulus
GO:0071310	0.56	cellular response to organic substance
GO:0061041	0.56	regulation of wound healing
GO:0051234	0.56	establishment of localization
GO:0048856	0.56	anatomical structure development
GO:0030335	0.56	positive regulation of cell migration
GO:0008360	0.56	regulation of cell shape
GO:0051094	0.55	positive regulation of developmental process
GO:0045595	0.55	regulation of cell differentiation
GO:0044765	0.55	single-organism transport
GO:0060284	0.54	regulation of cell development
GO:0051489	0.54	regulation of filopodium assembly
GO:0030036	0.54	actin cytoskeleton organization
GO:0010591	0.54	regulation of lamellipodium assembly
GO:0071702	0.53	organic substance transport
GO:0051246	0.53	regulation of protein metabolic process
GO:0051017	0.53	actin filament bundle assembly
GO:0035556	0.53	intracellular signal transduction
GO:0031324	0.53	negative regulation of cellular metabolic process
GO:0023051	0.53	regulation of signaling
GO:0010720	0.53	positive regulation of cell development
GO:0010646	0.53	regulation of cell communication
GO:0010605	0.53	negative regulation of macromolecule metabolic process
GO:1901700	0.52	response to oxygen-containing compound
GO:0048584	0.52	positive regulation of response to stimulus
GO:0032269	0.52	negative regulation of cellular protein metabolic process
GO:0019220	0.52	regulation of phosphate metabolic process
GO:0015031	0.52	protein transport
GO:0014070	0.52	response to organic cyclic compound
GO:0010769	0.52	regulation of cell morphogenesis involved in differentiation
GO:0009966	0.52	regulation of signal transduction
GO:2001046	0.51	positive regulation of integrin-mediated signaling pathway
GO:1901731	0.51	positive regulation of platelet aggregation
GO:1900026	0.51	positive regulation of substrate adhesion-dependent cell spreading
GO:1900025	0.51	negative regulation of substrate adhesion-dependent cell spreading
GO:0090527	0.51	actin filament reorganization
GO:0090315	0.51	negative regulation of protein targeting to membrane
GO:0071320	0.51	cellular response to cAMP
GO:0071277	0.51	cellular response to calcium ion
GO:0070560	0.51	protein secretion by platelet
GO:0051895	0.51	negative regulation of focal adhesion assembly
GO:0050732	0.51	negative regulation of peptidyl-tyrosine phosphorylation
GO:0048821	0.51	erythrocyte development
GO:0035585	0.51	calcium-mediated signaling using extracellular calcium source
GO:0035584	0.51	calcium-mediated signaling using intracellular calcium source
GO:0033137	0.51	negative regulation of peptidyl-serine phosphorylation
GO:0010801	0.51	negative regulation of peptidyl-threonine phosphorylation
GO:0010763	0.51	positive regulation of fibroblast migration

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0044424	0.98	intracellular part
GO:0044446	0.87	intracellular organelle part
GO:0044430	0.77	cytoskeletal part
GO:0043226	0.76	organelle
GO:0043229	0.73	intracellular organelle
GO:0044444	0.67	cytoplasmic part
GO:0016020	0.67	membrane
GO:0043232	0.65	intracellular non-membrane-bounded organelle
GO:0005856	0.64	cytoskeleton
GO:0043227	0.63	membrane-bounded organelle
GO:0005886	0.62	plasma membrane
GO:0043231	0.60	intracellular membrane-bounded organelle
GO:0044425	0.58	membrane part
GO:0044459	0.54	plasma membrane part
GO:0005829	0.54	cytosol
GO:0098590	0.53	plasma membrane region
GO:0044463	0.52	cell projection part
GO:0031988	0.51	membrane-bounded vesicle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	3b8cA	0.281	6.74	0.036	0.449	3.6.3.6	NA
2	0.060	3cf4A	0.272	6.68	0.051	0.432	1.2.99.2	NA
3	0.060	3b9jI	0.122	5.65	0.033	0.180	1.17.1.4 1.17.3.2	NA
4	0.060	2uv8G	0.267	7.29	0.022	0.457	2.3.1.86	NA
5	0.060	1nbwA	0.274	6.36	0.031	0.420	4.2.1.30	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

No Binding sites was predicted

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

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References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.