D-I-TASSER Q00G26

D-I-TASSER results for Q00G26

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q00G26
Protein Name: Perilipin-5
Gene Name: PLIN5

Input Sequence in FASTA format

>Q00G26 (463 residues)
MSEEEAAQIPRSSVWEQDQQNVVQRVVALPLVRATCTAVCDVYSAAKDRHPLLGSACRLA
ENCVCGLTTRALDHAQPLLEHLQPQLATMNSLACRGLDKLEEKLPFLQQPSETVVTSAKD
VVASSVTGVVDLARRGRRWSVELKRSVSHAVDVVLEKSEELVDHFLPMTEEELAALAAEA
EGPEVGSVEDQRRQQGYFVRLGSLSARIRHLAYEHSVGKLRQSKHRAQDTLAQLQETLEL
IDHMQCGVTPTAPACPGKVHELWGEWGQRPPESRRRSQAELETLVLSRSLTQELQGTVEA
LESSVRGLPAGAQEKVAEVRRSVDALQTAFADARCFRDVPAAALAEGRGRVAHAHACVDE
LLELVVQAVPLPWLVGPFAPILVERPEPLPDLADLVDEVIGGPDPRWAHLDWPAQQRAWE
AEHRDGSGNGDGDRMGVAGDICEQEPETPSCPVKHTLMPELDF

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q00G26-D1	1-463	463		MSEEEAAQIPRSSVWEQDQQNVVQRVVALPLVRATCTAVCDVYSAAKDRHPLLGSACRLAENCVCGLTTRALDHAQPLLEHLQPQLATMNSLACRGLDKLEEKLPFLQQPSETVVTSAKDVVASSVTGVVDLARRGRRWSVELKRSVSHAVDVVLEKSEELVDHFLPMTEEELAALAAEAEGPEVGSVEDQRRQQGYFVRLGSLSARIRHLAYEHSVGKLRQSKHRAQDTLAQLQETLELIDHMQCGVTPTAPACPGKVHELWGEWGQRPPESRRRSQAELETLVLSRSLTQELQGTVEALESSVRGLPAGAQEKVAEVRRSVDALQTAFADARCFRDVPAAALAEGRGRVAHAHACVDELLELVVQAVPLPWLVGPFAPILVERPEPLPDLADLVDEVIGGPDPRWAHLDWPAQQRAWEAEHRDGSGNGDGDRMGVAGDICEQEPETPSCPVKHTLMPELDF

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.597

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6mu1A	0.45	6.27	0.022	0.652	model1_6mu1A.pdb.gz
2	5vkqA	0.37	6.85	0.062	0.579	model1_5vkqA.pdb.gz
3	6ysgA	0.37	6.78	0.039	0.581	model1_6ysgA.pdb.gz
4	6bpqA	0.36	6.29	0.041	0.536	model1_6bpqA.pdb.gz
5	5wp6A	0.35	7.00	0.058	0.564	model1_5wp6A.pdb.gz
6	6ar6A	0.35	7.80	0.031	0.620	model1_6ar6A.pdb.gz
7	5owvC	0.35	6.43	0.045	0.505	model1_5owvC.pdb.gz
8	6co7A	0.35	6.91	0.034	0.549	model1_6co7A.pdb.gz
9	6vr4A	0.35	6.93	0.045	0.555	model1_6vr4A.pdb.gz
10	4r04A	0.35	7.41	0.040	0.581	model1_4r04A.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0019899	0.60	enzyme binding
GO:0035473	0.58	lipase binding
GO:0003824	0.52	catalytic activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.87	biological regulation
GO:0009987	0.81	cellular process
GO:0065008	0.79	regulation of biological quality
GO:0050789	0.75	regulation of biological process
GO:0080090	0.71	regulation of primary metabolic process
GO:0019216	0.70	regulation of lipid metabolic process
GO:0009894	0.70	regulation of catabolic process
GO:0009892	0.70	negative regulation of metabolic process
GO:0050995	0.69	negative regulation of lipid catabolic process
GO:0050794	0.69	regulation of cellular process
GO:0048518	0.68	positive regulation of biological process
GO:0048522	0.66	positive regulation of cellular process
GO:0044699	0.66	single-organism process
GO:0031323	0.66	regulation of cellular metabolic process
GO:0090207	0.65	regulation of triglyceride metabolic process
GO:0045834	0.65	positive regulation of lipid metabolic process
GO:0044092	0.65	negative regulation of molecular function
GO:0032879	0.65	regulation of localization
GO:0031329	0.65	regulation of cellular catabolic process
GO:0031325	0.65	positive regulation of cellular metabolic process
GO:0016043	0.65	cellular component organization
GO:0090208	0.64	positive regulation of triglyceride metabolic process
GO:0050996	0.64	positive regulation of lipid catabolic process
GO:0034389	0.64	lipid particle organization
GO:0031331	0.64	positive regulation of cellular catabolic process
GO:0031330	0.64	negative regulation of cellular catabolic process
GO:0010896	0.64	regulation of triglyceride catabolic process
GO:0010883	0.64	regulation of lipid storage
GO:0010866	0.64	regulation of triglyceride biosynthetic process
GO:0009966	0.64	regulation of signal transduction
GO:2000378	0.63	negative regulation of reactive oxygen species metabolic process
GO:0060193	0.63	positive regulation of lipase activity
GO:0060192	0.63	negative regulation of lipase activity
GO:0035359	0.63	negative regulation of peroxisome proliferator activated receptor signaling pathway
GO:0032000	0.63	positive regulation of fatty acid beta-oxidation
GO:0031999	0.63	negative regulation of fatty acid beta-oxidation
GO:0010897	0.63	negative regulation of triglyceride catabolic process
GO:0010890	0.63	positive regulation of sequestering of triglyceride
GO:0010867	0.63	positive regulation of triglyceride biosynthetic process
GO:0008152	0.58	metabolic process
GO:0044710	0.51	single-organism metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0044424	0.92	intracellular part
GO:0044444	0.81	cytoplasmic part
GO:0043229	0.80	intracellular organelle
GO:0005811	0.72	lipid particle
GO:0043227	0.68	membrane-bounded organelle
GO:0043231	0.67	intracellular membrane-bounded organelle
GO:0005737	0.55	cytoplasm

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2vdcF	0.268	6.82	0.067	0.421	1.4.1.13	NA
2	0.060	2vkzG	0.312	6.94	0.041	0.497	2.3.1.38 3.1.2.14	NA
3	0.060	3b8eC	0.234	6.31	0.048	0.350	3.6.3.9	NA
4	0.060	1f1oA	0.223	5.24	0.057	0.296	4.3.2.2	NA
5	0.060	2uv8G	0.311	6.89	0.033	0.492	2.3.1.86	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

No Binding sites was predicted

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.