D-I-TASSER P43694

D-I-TASSER results for P43694

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: P43694
Protein Name: Transcription factor GATA-4
Gene Name: GATA4

Input Sequence in FASTA format

>P43694 (442 residues)
MYQSLAMAANHGPPPGAYEAGGPGAFMHGAGAASSPVYVPTPRVPSSVLGLSYLQGGGAG
SASGGASGGSSGGAASGAGPGTQQGSPGWSQAGADGAAYTPPPVSPRFSFPGTTGSLAAA
AAAAAAREAAAYSSGGGAAGAGLAGREQYGRAGFAGSYSSPYPAYMADVGASWAAAAAAS
AGPFDSPVLHSLPGRANPAARHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNA
CGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMK
LHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASSNSSNATTSSSEE
MRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQT
SSKQDSWNSLVLADSHGDIITA

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	P43694-D1	1-442	442		MYQSLAMAANHGPPPGAYEAGGPGAFMHGAGAASSPVYVPTPRVPSSVLGLSYLQGGGAGSASGGASGGSSGGAASGAGPGTQQGSPGWSQAGADGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYSSGGGAAGAGLAGREQYGRAGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPAARHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.3913

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	3h6jA	0.78	3.47	0.103	0.919	model1_3h6jA.pdb.gz
2	1w8nA	0.65	4.34	0.062	0.808	model1_1w8nA.pdb.gz
3	2agsA	0.62	4.96	0.050	0.790	model1_2agsA.pdb.gz
4	1kitA	0.56	3.92	0.073	0.683	model1_1kitA.pdb.gz
5	2vk7A1	0.56	3.54	0.048	0.665	model1_2vk7A1.pdb.gz
6	2jkbA	0.55	3.87	0.065	0.665	model1_2jkbA.pdb.gz
7	1dilA	0.55	3.73	0.038	0.658	model1_1dilA.pdb.gz
8	2xcyA	0.53	3.92	0.063	0.645	model1_2xcyA.pdb.gz
9	3a0fA	0.50	4.74	0.034	0.656	model1_3a0fA.pdb.gz
10	3h71B	0.44	3.93	0.068	0.534	model1_3h71B.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:1901363	0.98	heterocyclic compound binding
GO:0097159	0.98	organic cyclic compound binding
GO:0003677	0.97	DNA binding
GO:0003700	0.93	transcription factor activity, sequence-specific DNA binding
GO:0044212	0.92	transcription regulatory region DNA binding
GO:0043565	0.91	sequence-specific DNA binding
GO:0003690	0.90	double-stranded DNA binding
GO:0000981	0.89	RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0000976	0.89	transcription regulatory region sequence-specific DNA binding
GO:0003682	0.86	chromatin binding
GO:0000977	0.86	RNA polymerase II regulatory region sequence-specific DNA binding
GO:0001228	0.85	transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0001085	0.85	RNA polymerase II transcription factor binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.97	cellular process
GO:0044699	0.96	single-organism process
GO:0044763	0.94	single-organism cellular process
GO:0048869	0.93	cellular developmental process
GO:0032502	0.86	developmental process
GO:0048856	0.85	anatomical structure development
GO:0044767	0.84	single-organism developmental process
GO:0048513	0.82	animal organ development
GO:0009653	0.81	anatomical structure morphogenesis
GO:0035295	0.74	tube development
GO:0009888	0.73	tissue development
GO:0048646	0.72	anatomical structure formation involved in morphogenesis
GO:0009887	0.72	organ morphogenesis
GO:0048565	0.70	digestive tract development
GO:0048468	0.70	cell development
GO:0045165	0.70	cell fate commitment
GO:0007507	0.69	heart development
GO:0065007	0.63	biological regulation
GO:0050789	0.62	regulation of biological process
GO:0050794	0.60	regulation of cellular process
GO:0080090	0.53	regulation of primary metabolic process
GO:0060255	0.53	regulation of macromolecule metabolic process
GO:0048518	0.53	positive regulation of biological process
GO:0031323	0.53	regulation of cellular metabolic process
GO:0048522	0.51	positive regulation of cellular process
GO:0006355	0.51	regulation of transcription, DNA-templated

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0005634	1.00	nucleus
GO:0032991	0.90	macromolecular complex
GO:0005667	0.89	transcription factor complex

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.067	1vcuB	0.564	3.61	0.054	0.670	3.2.1.18	NA
2	0.066	1cvmA	0.516	4.17	0.068	0.640	3.1.3.8	NA
3	0.066	3fw0A	0.484	4.09	0.060	0.597	4.3.2.5	221
4	0.066	2uvkB	0.490	4.01	0.052	0.602	5.1.3.-	NA
5	0.066	2g8sA	0.492	3.98	0.075	0.604	1.1.5.-	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	1w0oA	0.557	3.93	0.077	0.674	0.28	SIA	complex1.pdb.gz	228,230,284,285
2	0.01	2f0zA	0.555	3.50	0.066	0.654	0.16	ZMR	complex2.pdb.gz	60,110,111,168,228
3	0.01	2w68A	0.231	5.12	0.016	0.312	0.15	UUU	complex3.pdb.gz	316,317,318
4	0.01	2vc2A	0.510	4.40	0.056	0.647	0.33	180	complex4.pdb.gz	26,297,302,303,306
5	0.01	2a75A	0.615	5.00	0.042	0.790	0.22	FSI	complex5.pdb.gz	12,29,60,61,210,229
6	0.01	3ijeA	0.570	5.14	0.045	0.762	0.26	UUU	complex6.pdb.gz	296,298,303,304,306
7	0.01	2f25B	0.558	3.63	0.058	0.665	0.14	DAN	complex7.pdb.gz	28,59,60,275

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.