D-I-TASSER P33076-D1

D-I-TASSER results for P33076-D1

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Input Sequence in FASTA format

>P33076 (1078 residues)
MRCLAPRPAGSYLSEPQGSSQCATMELGPLEGGYLELLNSDADPLCLYHFYDQMDLAGEE
EIELYSEPDTDTINCDQFSRLLCDMEGDEETREAYANIAELDQYVFQDSQLEGLSKDIFK
HIGPDEVIGESMEMPAEVGQKSQKRPFPEELPADLKHWKPAEPPTVVTGSLLVRPVSDCS
TLPCLPLPALFNQEPASGQMRLEKTDQIPMPFSSSSLSCLNLPEGPIQFVPTISTLPHGL
WQISEAGTGVSSIFIYHGEVPQASQVPPPSGFTVHGLPTSPDRPGSTSPFAPSATDLPSM
PEPALTSRANMTEHKTSPTQCPAAGEVSNKLPKWPEPVEQFYRSLQDTYGAEPAGPDGIL
VEVDLVQARLERSSSKSLERELATPDWAERQLAQGGLAEVLLAAKEHRRPRETRVIAVLG
KAGQGKSYWAGAVSRAWACGRLPQYDFVFSVPCHCLNRPGDAYGLQDLLFSLGPQPLVAA
DEVFSHILKRPDRVLLILDGFEELEAQDGFLHSTCGPAPAEPCSLRGLLAGLFQKKLLRG
CTLLLTARPRGRLVQSLSKADALFELSGFSMEQAQAYVMRYFESSGMTEHQDRALTLLRD
RPLLLSHSHSPTLCRAVCQLSEALLELGEDAKLPSTLTGLYVGLLGRAALDSPLAFPSFL
LQCFLGALWLALSGEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLIFQPPARCLGA
LLGPSAAASVDRKQKVLARYLKRLQPGTLRARQLLELLHCAHEAEEAGIWQHVVQELPGR
LSFLGTRLTPPDAHVLGKALEAAGQDFSLDLRSTGICPSGLGSLVGLSCVTRFRAALSDT
VALWESLQQHGETKLLQAAEEKFTIEPFKAKSLKDVEDLGKLVQTQRTRSSSEDTAGELP
AVRDLKKLEFALGPVSGPQAFPKLVRILTAFSSLQHLDLDALSENKIGDEGVSQLSATFP
QLKSLETLNLSQNNITDLGAYKLAEALPSLAASLLRLSLYNNCICDVGAESLARVLPDMV
SLRVMDVQYNKFTAAGAQQLAASLRRCPHVETLAMWTPTIPFSVQEHLQQQDSRISLR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 720 740 760 780 800 820 840 860 880 900 920 940 960 980 1000 1020 1040 1060 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MRCLAPRPAGSYLSEPQGSSQCATMELGPLEGGYLELLNSDADPLCLYHFYDQMDLAGEEEIELYSEPDTDTINCDQFSRLLCDMEGDEETREAYANIAELDQYVFQDSQLEGLSKDIFKHIGPDEVIGESMEMPAEVGQKSQKRPFPEELPADLKHWKPAEPPTVVTGSLLVRPVSDCSTLPCLPLPALFNQEPASGQMRLEKTDQIPMPFSSSSLSCLNLPEGPIQFVPTISTLPHGLWQISEAGTGVSSIFIYHGEVPQASQVPPPSGFTVHGLPTSPDRPGSTSPFAPSATDLPSMPEPALTSRANMTEHKTSPTQCPAAGEVSNKLPKWPEPVEQFYRSLQDTYGAEPAGPDGILVEVDLVQARLERSSSKSLERELATPDWAERQLAQGGLAEVLLAAKEHRRPRETRVIAVLGKAGQGKSYWAGAVSRAWACGRLPQYDFVFSVPCHCLNRPGDAYGLQDLLFSLGPQPLVAADEVFSHILKRPDRVLLILDGFEELEAQDGFLHSTCGPAPAEPCSLRGLLAGLFQKKLLRGCTLLLTARPRGRLVQSLSKADALFELSGFSMEQAQAYVMRYFESSGMTEHQDRALTLLRDRPLLLSHSHSPTLCRAVCQLSEALLELGEDAKLPSTLTGLYVGLLGRAALDSPLAFPSFLLQCFLGALWLALSGEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLIFQPPARCLGALLGPSAAASVDRKQKVLARYLKRLQPGTLRARQLLELLHCAHEAEEAGIWQHVVQELPGRLSFLGTRLTPPDAHVLGKALEAAGQDFSLDLRSTGICPSGLGSLVGLSCVTRFRAALSDTVALWESLQQHGETKLLQAAEEKFTIEPFKAKSLKDVEDLGKLVQTQRTRSSSEDTAGELPAVRDLKKLEFALGPVSGPQAFPKLVRILTAFSSLQHLDLDALSENKIGDEGVSQLSATFPQLKSLETLNLSQNNITDLGAYKLAEALPSLAASLLRLSLYNNCICDVGAESLARVLPDMVSLRVMDVQYNKFTAAGAQQLAASLRRCPHVETLAMWTPTIPFSVQEHLQQQDSRISLR
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCSSSSCCCCCCCCCCCCSSSCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSCCCCCCCCCCCCCCCCCCCCCCCCSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCSSSSSSCCCCCCCCCCCSSSCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCSSSSSSCCCCCCHHHHHHHHHHHHHCCCCCCSSSSSSSSCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCSSSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCSSSSCCCCCCCCCCCCCCCSSSSSSCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHSSSCCCCCCHHHHHHHHHHHHHCCCCSSSSCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCSSCCCCCCCCCCCHHHHHHHHHHHHHCCCCCSSSCCCCCCCHHHHHHHHHHHHCCCCCCCSSSCCCCCCCHHHHHHHHHHHHHCCCCCSSSCCCCCCCHHHHHHHHHHHHHCCCCCSSSCCCCCCCHHHHHHHHHHHHHCCCC
Confidence	9765778889877689998755322457887427887507998401332036677788753010478874421368999986654267036777765677776531132213454331346662333212433234423556578998888877555787889652212224688777654556777665667888877775656566788877666667888887400358898877666777777777650453677776667899987746789999888788888888866677888765223556221567876642100133113210678999999888750688777111432111223013456664210220247763222344437887613677777876239999858988599999999999981976542299998412216676435899999986889876336799999855894999971631267776777676668865566599999999861327888099980874003566445884999927999999999999953688578899999999836799999779999999999999998527777789857999998778763367756652246889999999874552111234455555530110102124433202444124442134431131678899999999999998751132114677665443344432678998988510115302576774899999999985376414322124789888888876654112455666778999999986256677876523431244420110122344421023424689999998764330144553058977358999999998616777612213267899989999999999964899888978899996899999999982058976789777999978999999999973899987978799898999999999996199887897549989999999999752211369
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 720 740 760 780 800 820 840 860 880 900 920 940 960 980 1000 1020 1040 1060 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MRCLAPRPAGSYLSEPQGSSQCATMELGPLEGGYLELLNSDADPLCLYHFYDQMDLAGEEEIELYSEPDTDTINCDQFSRLLCDMEGDEETREAYANIAELDQYVFQDSQLEGLSKDIFKHIGPDEVIGESMEMPAEVGQKSQKRPFPEELPADLKHWKPAEPPTVVTGSLLVRPVSDCSTLPCLPLPALFNQEPASGQMRLEKTDQIPMPFSSSSLSCLNLPEGPIQFVPTISTLPHGLWQISEAGTGVSSIFIYHGEVPQASQVPPPSGFTVHGLPTSPDRPGSTSPFAPSATDLPSMPEPALTSRANMTEHKTSPTQCPAAGEVSNKLPKWPEPVEQFYRSLQDTYGAEPAGPDGILVEVDLVQARLERSSSKSLERELATPDWAERQLAQGGLAEVLLAAKEHRRPRETRVIAVLGKAGQGKSYWAGAVSRAWACGRLPQYDFVFSVPCHCLNRPGDAYGLQDLLFSLGPQPLVAADEVFSHILKRPDRVLLILDGFEELEAQDGFLHSTCGPAPAEPCSLRGLLAGLFQKKLLRGCTLLLTARPRGRLVQSLSKADALFELSGFSMEQAQAYVMRYFESSGMTEHQDRALTLLRDRPLLLSHSHSPTLCRAVCQLSEALLELGEDAKLPSTLTGLYVGLLGRAALDSPLAFPSFLLQCFLGALWLALSGEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLIFQPPARCLGALLGPSAAASVDRKQKVLARYLKRLQPGTLRARQLLELLHCAHEAEEAGIWQHVVQELPGRLSFLGTRLTPPDAHVLGKALEAAGQDFSLDLRSTGICPSGLGSLVGLSCVTRFRAALSDTVALWESLQQHGETKLLQAAEEKFTIEPFKAKSLKDVEDLGKLVQTQRTRSSSEDTAGELPAVRDLKKLEFALGPVSGPQAFPKLVRILTAFSSLQHLDLDALSENKIGDEGVSQLSATFPQLKSLETLNLSQNNITDLGAYKLAEALPSLAASLLRLSLYNNCICDVGAESLARVLPDMVSLRVMDVQYNKFTAAGAQQLAASLRRCPHVETLAMWTPTIPFSVQEHLQQQDSRISLR
Prediction	6413233541431443624220030411214201030020312000001011414243543240234232220314202300130444542340233033014101424416424442053022443224224234534541453545654244244443443343133311333444332222242332345434444442544444324423441422424443242323425044411435434342320011235244444434213221513242454345344343254425524522344244356464425534445424541561442044113402542455242243000301101130443345333441432434444445431340033256664444110000212200000000000010004231210100000001103424441001200141003365234301420153130000000000002130515544314215443303200120033300330100000101043232144031101000014720230011003324344103200310351310000000000000000001200444543430300000111111221244423124322310111010000110222000111111233311221000101111000001121202220211100110220110001002202133210220010000000011110022023203330301212022200200020021120100000010000010020111001011111001000000210332111000101221000110221001100000000102111200000020021011011001110200134002000300330320230102101403011400420040034034344434240301040042104004411230344202303000400210040034034244434340301040031004003403414433444140244005204624642547
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

Hydrogen bond networks

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank

PDB
hit

ID1

ID2

Cov

Norm.
Zscore

Download
alignment

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820                 840                 860                 880                 900                 920                 940                 960                 980                1000                1020                1040                1060

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SS
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCSSSSCCCCCCCCCCCCSSSCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSCCCCCCCCCCCCCCCCCCCCCCCCSSSCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCSSSSSSCCCCCCCCCCCSSSCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCSSSSSSCCCCCCHHHHHHHHHHHHHCCCCCCSSSSSSSSCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCSSSSSSSCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCSSSSCCCCCCCCCCCCCCCSSSSSSCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHSSSCCCCCCHHHHHHHHHHHHHCCCCSSSSCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCSSCCCCCCCCCCCHHHHHHHHHHHHHCCCCCSSSCCCCCCCHHHHHHHHHHHHCCCCCCCSSSCCCCCCCHHHHHHHHHHHHHCCCCCSSSCCCCCCCHHHHHHHHHHHHHCCCCCSSSCCCCCCCHHHHHHHHHHHHHCCCC
MRCLAPRPAGSYLSEPQGSSQCATMELGPLEGGYLELLNSDADPLCLYHFYDQMDLAGEEEIELYSEPDTDTINCDQFSRLLCDMEGDEETREAYANIAELDQYVFQDSQLEGLSKDIFKHIGPDEVIGESMEMPAEVGQKSQKRPFPEELPADLKHWKPAEPPTVVTGSLLVRPVSDCSTLPCLPLPALFNQEPASGQMRLEKTDQIPMPFSSSSLSCLNLPEGPIQFVPTISTLPHGLWQISEAGTGVSSIFIYHGEVPQASQVPPPSGFTVHGLPTSPDRPGSTSPFAPSATDLPSMPEPALTSRANMTEHKTSPTQCPAAGEVSNKLPKWPEPVEQFYRSLQDTYGAEPAGPDGILVEVDLVQARLERSSSKSLERELATPDWAERQLAQGGLAEVLLAAKEHRRPRETRVIAVLGKAGQGKSYWAGAVSRAWACGRLPQYDFVFSVPCHCLNRPGDAYGLQDLLFSLGPQPLVAADEVFSHILKRPDRVLLILDGFEELEAQDGFLHSTCGPAPAEPCSLRGLLAGLFQKKLLRGCTLLLTARPRGRLVQSLSKADALFELSGFSMEQAQAYVMRYFESSGMTEHQDRALTLLRDRPLLLSHSHSPTLCRAVCQLSEALLELGEDAKLPSTLTGLYVGLLGRAALDSPLAFPSFLLQCFLGALWLALSGEIKDKELPQYLALTPRKKRPYDNWLEGVPRFLAGLIFQPPARCLGALLGPSAAASVDRKQKVLARYLKRLQPGTLRARQLLELLHCAHEAEEAGIWQHVVQELPGRLSFLGTRLTPPDAHVLGKALEAAGQDFSLDLRSTGICPSGLGSLVGLSCVTRFRAALSDTVALWESLQQHGETKLLQAAEEKFTIEPFKAKSLKDVEDLGKLVQTQRTRSSSEDTAGELPAVRDLKKLEFALGPVSGPQAFPKLVRILTAFSSLQHLDLDALSENKIGDEGVSQLSATFPQLKSLETLNLSQNNITDLGAYKLAEALPSLAASLLRLSLYNNCICDVGAESLARVLPDMVSLRVMDVQYNKFTAAGAQQLAASLRRCPHVETLAMWTPTIPFSVQEHLQQQDSRISLR

6b5bA

0.13

0.11

3.90

1.05

MapAlign

---------------QVAKSQEEEEHKERMKMKKGFNSQMRSEAKRLKTFETYDTFRSWTPQEMAAAGFYHTGVKLGVQCILFGNSLRKLPIERHKARLESFEDWAHGTSPRVLSAAGFVFTGKRDTV--------------------------------------------QCFSCGGSLGNWEEGDDPWKEHAKWFPKCEFLQSKKSDSVFANEELRMDMFKDWPQESPVGVEALVRAGFFYTGKKDIVRCFS-----------------------------------CGGCLEKWAEGDDPMEDHIKFFPECVFLQTLKSSAEVIPTLQSQFQEARSLSEQLRDNCSSLGTDHLLSCVSIISKHI-------------------SQPVQEALTIPEVFS--------NLNSVMCVEGETGSGKTTFLKRIAFLWASGCLYRFQLVFYLSLSSITP---DQGLANIICAQLLGAGISEVCLSSSIQQLQHQVLFLLDDYSG------------------LASLPQALHTLITKNYLSRTCLLIAVHT-NRVRDIRLYLGTSLEIQEFPFYNTVSVLRKFFSH--DIICVEKLIIYFIDNKDLQGVYKTPLFVAAVCTDWIQN------ASAKFQDVTLFQSYMQYLSKATAEPLQATVSSCGQLALTGLFLTTLLMSKFTAQRLRPVYRFLGPLFQEFLAAVRLTELLSSDDQDLGLYYL---RQIDSPLKAINSFNIFLYYLWLVSPESSSSFVSEHLLRLYENDISEGYWKLIPKLEVQVNNTDAAALLQVLMEVFSAS-QSIEFRLFNSSGFLESICPALEL-SKASVTKCSMSRLELSRAEQELHHMEKLSIRTSTESDLSKLVKFIQNFPNLHVFHLKCDFLSNCESLMAVLASCKKLREIEFSGRCFE----AMTFVNILPNFVSLKILNLK--DQQFPDKETSEKFAQALGSLRNLEELLVPTGDGIHQVAKLIVRQCLQL-PCLRVLTFHDI-LDDDSVIEIARAATGFQKLENLDISMNKITEEGYRNFFQALDNLPNLQELNICRNQVQKALGQCVIRLHMLSWL-

5irlA

0.25

0.16

4.94

3.60

SPARKS-K

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EAAACKKYMSKLRTIVAAQSRFLNLCLEDIYTENTL--------------------EVRTAA---LGLEELF---------SDADTVLVVGEAGSGKSTLLQQVHLLWATGQDFQFLFVFPFSCRQLQCVARPLSVMTLLFEHCCWPDVGQQDVFQFLLDHPDRILLTFDGFDEFKFKFTDHER--HCSPTDPTSVQTLLFNLLQGNLLKNARKVLTSRPDAVSAFLRKYVRTEFNLKGFSEEGIELYLRKCHREP---GVADRLIHLLQTTSALHGLCHLPVFSWMVSKCHQELLLQDGGS--PKTTTDMYLLILQHFLRHAPLEFLHITFQCFLAAFYLVLSTDVPTASLRYLFTVAALLQKTEPHNLQITAAFLAGLLSREHRDLLAACQASE--RSLLRRRACARWCLARSLHKIPAMPGFLWLIRSLYEMQEERLAQEAVRGLNVEHKLTFCGVGPAECAALAFVLRHLRRPVALQLDHNSVGDIGVEQLLPLGACKDNNISDRGICKLIEHALHCEQLQKLALFNNKLTD--------GCAHSVAQLLACK----------------QNFLALRLGN-NHITAEGAQVLAEGLRDNSSLQFLGF---WGNKVGDKGAQALAEALSDHQSLKWLSLVGNNIGSVGAQALASMLEKNVA-LEELCLAANHLQDAGVCSLAEGLKRNSSLKVLKLSNNCITFVGAEALLQALASNDTILEVWLRGNPFSPEEMEALSHRDSRLLL-

6b5bA

0.15

0.12

4.17

2.22

SPARKS-K

---------------------------------------------------------------------------------------------------------------------------AFQVAKSQEEEEHKERMKMKKGF-NSQMRSEAKRLKETYDTFRSAAAGFYHTGVKLGCCSLILFGNSLRKLPIERHKKLREEADWPFYAHGTSPR--VLSAAGF-----VFTGKRDTVQCFSCGG-------SLGVFANEKDWPQESPVGVEALVRTGKKDIVRCFSCGGCLEKWAEDDPMEDHIKFFPECVFLQTLKSSAEVIPLQSQF--QEARSLSEQLRDNYTKSLGTDHLLSCDVSIISKHISQ-----------------PVQEALTIPEVFSNLNS--------VMCVEGETGSGKTTFLKRIAFLWASGLLYRFQLVFYLSLSSITPDQ---GLANIICAQLLGAGCISEVCLSSSIQQLQQVLFLLDDYSGLA-SLP-----------------QALHTLITKNYLSRTCLLIAVHTNRVR-DIRLYLGTSLEIQEFPFYNTVSVLRKFFSH--DIICVEKLIIYFIDNKDLQGVYKTPLFVAAVCTDWIQYLSLATAEPLQATVSSCGQLALTGLFSRPVYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQIDSPLKAINSFNIFLYYVSSKAAPTVVSHLLQLVDEKELHPQTFLWFQFVRGLWLVSSSSFVSEHLLRLALIFAYESCSPFILQFLRGKTLALRVLNLPESLLSICPALELSKASVKCSMSRLELSRAEQELLLTLPALQLPEQLFHNLHVLPGEFSTESDLSKLVKFIQNFPNLHLKCDFLSNCESLMAVLASCKRCFEAMTFVNILPNFVSLKILNLKDQQFPDKETSEKFAQALGSLRNLEELLVPTG---DGIHQVAKLIVRQCLQLPCLRVLTFHDI-LDDDSVIEIARAATSGGFQLENLDISMNKITEEGYRNFFQALDNLPNLQELNICRNQVQATTVKALGQCVSRLPSLIRLHMLSWLLDEEDHPQSKRLII-----

5irlA

0.26

0.16

4.82

1.55

MapAlign

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AACKKYMSKLRTIVASRFLNLCLEDIYTENTLEV-----------------------RTAALGLEELF---------SDADTVLVVGEAGSGKSTLLQQVHLLWATGQDFEFLFVFPFSCRQLQCVARPLSVMTLLFEHCCWPDVGQQDVFQFLLDHPDRILLTFDGFDEFKFKFTD--HERHCSPTDPTSVQTLLFNLLQGNLLKNARKVLTSRPDAVSAFLRKYVRTEFNLKGFSEEGIELYLRKCH---REPGVADRLIHLLQTTSALHGLCHLPVFSWMVSKCHQELLLQDGGS--PKTTTDMYLLILQHFLVQAPLEFLHITFQCFLAAFYLVLSTDVPTASLRYLFTVAALLQKTEPHNLQITAAFLAGLLSREHRDLLAACQERSLLRRRACARWCLARSLHKHFRSIPAMPGFLWLIRSLYEMQEERLAQEAVGLNVEHLKLTFCGVGPAECAALAFVLRHLRRPVALQLDHNSVGDIGVEQLL--PCLGACKALYLRD-------------------------------NNISDRGICKL-------------IEHALHCEQLQKLALFNNKLT-DGCAHSVAQLLACKQNFLALRLG--NN-HITAEGAQVLAEGLRDNSSLQFLGFWGNKVGDKGAQALAEALSDH-QSLKWLSLVGNNIGSVGAQALASMLEKNVALEELCLAANHLQDAGVCSLAEGLKRNSSLKVLKLSNNCITFVGAEALLEVWLRGNPF

6b5bA

0.16

0.13

4.47

1.68

MUSTER

-----------------------------------------------------------------------------AFQVAKSQE--EEEHKERMKM---------NSQMRSEAKRLKTFETYDTFRSWTPQEMAAAGVKLG-GNSLRKLPIE-RHKKLRESPRVLSAAGFVFTGKRDTVQCFSCGGSLGNWEEGDDAKWFPKCEFLQSKKSDSVF------ANEELRMDMFKDWPQE---SPVGVEALVRAGFFYTGKKDIVRCFSCGGCLEKWAEGD-------DPMEDHIKFFPECVF---------------LQTLKSSAEVIPTLQSQFQEARSLSEQLRDNYTKATFRDHLLSCDVSIISKHISQ-----------------PVQEALTIPEVFSNLN--------SVMCVEGETGSGKTTFLKRIAFLWASGCCPRFQLVFYLSLSSIT---PDQGLANIICAQLLGAGCISEVCLSSSIQQQHQVLFLLDDYSGLA------------------SLPQALHTLITKNYLSRTCLLIAVHTNRVRDI-RLYLGTSLEIQEFPFYNTVSVLRKFFSHDI--ICVEKLIIYFIDNKDLQGVYKTPLFVAAVCTDWIQN----ASAQDKFQDVTLFQSYMQYLSLKYKYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQIDSPLKAINSFNIFLYYVSSKAAPTVVSHLLQLVDEKKLHPQTFLWFQFVRGLWLVSVSEHLLRLALIFAYESNTVECSPFILQFLRGKLRVLNDAADQALLQVLMEVFSASQSVTKCSMSRLELSRAEQELLLTLPLPEQLFHNLHKFLGLPGEFTESDLSKLVKFIQNFPNLHVFKCDFLSNCESLMAVLASSGRCFEAMTFVNILPNFVSLKILNLKDQQFPDKETSEKFAQALGSLRNLEELL---VPTGDGIHQVAKLIVRQCLQLPCLRVLTFH-DILDDDSVIEIARAATSGGQKLENLDISMNKITEEGYRNFFQALDNLPNLQELNICRNQVQATTVKALGQCVSRLPSLIRLHMLSWLLDEEDMKVINDKERHPQSK

5irlA

0.25

0.16

4.89

1.07

CEthreader

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EAAACKKYMSKLRTIVAAQSRFLNLCLEDIYTENTLEV-----------------------RTAALGLEELFS---------DADTVLVVGEAGSGKSTLLQQVHLLWATGQDFQFLFVFPFSCRQLQCVARPLSVMTLLFEHCCWPDVGQQDVFQFLLDHPDRILLTFDGFDEFKFK--FTDHERHCSPTDPTSVQTLLFNLLQGNLLKNARKVLTSRPDAVSAFLRKYVRTEFNLKGFSEEGIELYLRKCHREP---GVADRLIHLLQTTSALHGLCHLPVFSWMVSKCHQELLLQDG--GSPKTTTDMYLLILQHFLRHAPLEFLHITFQCFLAAFYLVLSTDVPTASLRYLFTVAALLQKTEPHNLQITAAFLAGLLSREHRDLLAACQASER--SLLRRRACARWCLARSLHKHFAMPGFLWLIRSLYEMQEERLAQEAVRGLNVELKLTFCGVGPAECAALAFVLRHLRRPVALQLDHNSVGDIGVEQLLPCLG--ACKALYLRDNNISDRGICKLIEHALHCEQLQKLALFNNKLTDGCAHSVAQLLACK----------------QNFLALRLG-NNHITAEGAQVLAEGLRDNSSLQFLG---FWGNKVGDKGAQALAEALSDHQSLKWLSLVGNNIGSVGAQALASMLEKN-VALEELCLAANHLQDAGVCSLAEGLKRNSSLKVLKLSNNCITFVGAEALLQALASNDTILEVWLRGNPFSPEEMEALSHRDSRLLL-

6b5bA

0.15

0.12

4.00

2.40

HHsearch

--------------------------------------------------------------------------AFQ---VAKSQE----------------------------------------------EEEHK--ERMKKKGFNSQMRSEAKRLKTFETPQEMAAAGFYHTGVKLGVSLILFLPERHKKLPFYAHGGKRDTVQCFSCHAKWFPKCEFLQSKKVFAMDMFKDWPQESPV--GVEALVRAGFFYTGKKDIV-RCFSCGGCLEKWA----------------EG-----DDPMEDHIKF------FP-ECVFLQTLAEVIPTLFQEARSLSEQLRDNYTKTFRHDHLLSCDVSIISKHIS-----------------QPVQEALTIPEVFSNL--------NSVMCVEGETGSGKTTFLKRIAFLWASGCCLYFQLVFYLSLSSITP---DQGLANIICAQLLGAGCISEVCLSSSIQQQHQVLFLLDDYSGLA------------------SLPQALHTLITKNYLSRTCLLIAVHTNRVRDIR-LYLGTSLEIQEFPFYNTVSVLRKFFSHDI--ICVEKLIIYFIDNKDLQGVYKTPLFVAAVCTDWIQ-----NASADKFQDVTLFQSYMQYLSLKYVYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQIDSPINSFNIFLYYVSSHSSKAAPTVVSHLLQLVDEKYMHPQTFLWFQFVRGLWLVSPELRLALIFAYESPFLRSAQDEEIIKNYENDICKINNDAADQALLQVICPALELSKSVTKCSMSRLELSRAEQELLLTLPAL---QSLEVSETQLPGEFPNLHHMEKLSIRFPNLHVFHLKCDFLSNCESLMAVLKLREIEFSGRCFVNILPNFVSLKILNLKDQQFPDKETSEKFAQALGSLRNLEELLV---PTGDGIHQVAKLIVRQCLQLPCLRVLTFH-DILDDDSVIEIARAATGFQ-KLENLDISMNKITEEGYRNFFQALDNLPNLQELNICPGRIQVQAVKALGQCVSRLPSLIRLHMLSWLLDEEDHPQSKRLII-----

5irlA

0.25

0.16

4.99

2.45

MUSTER

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EAAACKKYMSKLRTIVAAQSRFLNLCLEDIYTENTLEVRT-----------------------AALGLEELFS---------DADTVLVVGEAGSGKSTLLQQVHLLWATGQDFQFLFVFPFSCRQLQCVARPLSVMTLLFEHCCWPDVGQQDVFQFLLDHPDRILLTFDGFDEFKFK--FTDHERHCSPTDPTSVQTLLFNLLQGNLLKNARKVLTSRPDAVSAFLRKYVRTEFNLKGFSEEGIELYLRKCHREPG---VADRLIHLLQTTSALHGLCHLPVFSWMVSKCHQELLLQDG--GSPKTTTDMYLLILQHFLRHAPLEFLHITFQCFLAAFYLVLSTDVPTASLRYLFTVAALLQKTEPHNLQITAAFLAGLLSREHRDLLAACQASER--SLLRRRACARWCLARSLHKIPAMPGFLWLIRSLYEMQEERLAQEAVRGLNVELKLTFCGVGPAECAALAFVLRHLRRPVALQLDHNSVGDIGVEQLLPCLGA---KALYLRDNNI-------SDRGICKLIEHALHCEQLQKLALFNNKLTDGCAHS---------VAQLLACKQNFLALRLG-NNHITAEGAQVLAEGLRDNSSLQFLG---FWGNKVGDKGAQALAEALSDHQSLKWLSLVGNNIGSVGAQALASMLEKNV-ALEELCLAANHLQDAGVCSLAEGLKRNSSLKVLKLSNNCITFVGAEALLQALASNDTILEVWLRGNPFSPEEMEALSHRDSRLLL-

6b5bA

0.16

0.14

4.53

1.40

FFAS-3D

-----------RMKMKKGFNS--QMRS---EAKRLKTFERSWTP-------QEMAAAG-----FYHTGVKLGVQCFCCSLILFGNSLRKLPIERHKKLARLESFEDWPFYAHGTSPRVLSAAGFDTVQCFNWEEGDD-PWKEHAKWFPK-------------------CEFLQSKKSDSVFANEELRMDMFKD--WPQESPVGVEALVRA----------------------------GFFY-----TGKKDIV----------RCFSCGGCL---------------------EKWAEGDDPMEDHIKFFPECVFLQTLKSSAEVIPTLQSQF-QEARSLSEQLRD-----------NYTKATFRHMNLPEVCSSLGTDHLLSCHISQPVQEALTIPEVF--------SNLNSVMCVEGETGSGKTTFLKRIAFLWASGCCPRFQLVFYLS---LSSITPDQGLANIICAQLLGAGGSEVCLSSSIQQLQHQVLFLLDDYSGLASL------------------PQALHTLITKNYLSRTCLLIAVHTNRVRDI-RLYLGTSLEIQEFPFYNTVSVLRKFFSHDI--ICVEKLIIYFIDNKDLQGVYKTPLFVAAVCTDWIQNASKATAEPLQATVSSCGQLALTGLFSRPVYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQIDSPLKAINSFNIFLYYVSSKAAPTVVSHLLQLVDEKESLENLRLALIFAYESNTVAECSPFILQFLEEIIKNYENDISEGYWKLSPKPCKIPKDAADQALLQVLMEVFSASQSVTKCSMSRLELSRAEQELLLTLPALQQLFHNLHKFLGLPGEFPNLHHMKLVKFIQNNLHVFHLKCDFLSNCESLMAVLAFSGRCFEAMTFVNILPNFVSLKILNLKDQQFPDKETSEKFAQALGSLRNLEELL---VPTGDGIHQVAKLIVRQCLQLPCLRVLTFHDI-LDDDSVIEIARAATSGGQKLENLDISMNKITEEGYRNFFQALDNLPNLQELNICRNQVQATTVKALGQCVSRLPSLIRLHMLSWLLDEEDMKVINDVKERHQSK

5irlA

0.26

0.16

4.93

3.71

HHsearch

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EAAACKKYMSKLRTIVAAQSRFLNLCLEDIYTENTLEVR-----------------------TAALGLEELFS---------DADTVLVVGEAGSGKSTLLQQVHLLWATGQDFQFLFVFPFSCRQLQCVARPLSVMTLLFECWPDVG--QQDVFQFLLDHPDRILLTFDGFDEFKFK--FTDHERHCSPTDPTSVQTLLFNLLQGNLLKNARKVLTSRPDAVSAFLRKYVRTEFNLKGFSEEGIELYLRKCHREP---GVADRLIHLLQTTSALHGLCHLPVFSWMVSKCHQELLLQDG--GSPKTTTDMYLLILQHFLRHAPLEFLHITFQCFLAAFYLVLSTDVPTASLRYCSTVAALLQKTEPHNLQITAAFLAGLLSREHRDLLAACQASER--SLLRRRACARWCLARSLHKIPAMPGFLWLIRSLYEMQEERLAQEAVRGLNVEHKLTFCGVGPAECAALAFVLRHLRRPVALQLDHNSVGDIGVEQLLP--CLGACKALYLRDNNIS----------------------------DRGICKLIEHAQLQKLALFNNSVAQLLACKQNFLALRLG-NNHITAEGAQVLAEGLRDNSSLQFLGF---WGNKVGDKGAQALAEALSDHQSLKWLSLVGNNIGSVGAQALASMLEKNV-ALEELCLAANHLQDAGVCSLAEGLKRNSSLKVLKLSNNCITFVGAEALLQALASNDTILEVWLRGNPFSPEEMEALSHRDSRLLL-

(a)	ID1 is the number of template residues identical to query divided by number of aligned residues.
(b)	ID2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e)	Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f)	Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.5357

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6b5bA	0.81	2.07	0.150	0.834	model1_6b5bA.pdb.gz
2	4kxfK	0.39	5.13	0.078	0.456	model1_4kxfK.pdb.gz
3	1vt4I	0.38	8.49	0.077	0.560	model1_1vt4I.pdb.gz
4	6jxaA	0.36	8.63	0.050	0.541	model1_6jxaA.pdb.gz
5	6yufD	0.36	8.84	0.044	0.548	model1_6yufD.pdb.gz
6	6wg3E	0.34	8.62	0.061	0.513	model1_6wg3E.pdb.gz
7	6z2wE	0.34	8.43	0.039	0.502	model1_6z2wE.pdb.gz
8	6yvuD	0.34	8.04	0.055	0.490	model1_6yvuD.pdb.gz
9	6qj3A	0.34	8.23	0.065	0.492	model1_6qj3A.pdb.gz
10	5t8vA	0.34	9.14	0.039	0.526	model1_5t8vA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0097159	0.65	organic cyclic compound binding
GO:1901363	0.64	heterocyclic compound binding
GO:0003676	0.53	nucleic acid binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.92	biological regulation
GO:0050789	0.91	regulation of biological process
GO:0050794	0.87	regulation of cellular process
GO:0019222	0.80	regulation of metabolic process
GO:0048518	0.79	positive regulation of biological process
GO:0060255	0.77	regulation of macromolecule metabolic process
GO:0048522	0.77	positive regulation of cellular process
GO:0031323	0.76	regulation of cellular metabolic process
GO:0080090	0.75	regulation of primary metabolic process
GO:0009987	0.75	cellular process
GO:0050896	0.73	response to stimulus
GO:0009893	0.73	positive regulation of metabolic process
GO:0010604	0.71	positive regulation of macromolecule metabolic process
GO:0031325	0.69	positive regulation of cellular metabolic process
GO:0010468	0.68	regulation of gene expression
GO:0006355	0.66	regulation of transcription, DNA-templated
GO:0048519	0.64	negative regulation of biological process
GO:0042221	0.64	response to chemical
GO:0010033	0.63	response to organic substance
GO:0048523	0.61	negative regulation of cellular process
GO:0010628	0.61	positive regulation of gene expression
GO:0010557	0.61	positive regulation of macromolecule biosynthetic process
GO:0045893	0.60	positive regulation of transcription, DNA-templated
GO:0044699	0.58	single-organism process
GO:0006357	0.58	regulation of transcription from RNA polymerase II promoter
GO:0045944	0.57	positive regulation of transcription from RNA polymerase II promoter
GO:0031324	0.52	negative regulation of cellular metabolic process
GO:0010605	0.50	negative regulation of macromolecule metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0044424	0.89	intracellular part
GO:0043226	0.53	organelle
GO:0043229	0.50	intracellular organelle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2r7oA	0.248	9.38	0.042	0.392	2.7.7.48	557,560
2	0.060	2vumA	0.237	9.71	0.034	0.382	2.7.7.6	NA
3	0.060	3btaA	0.265	9.46	0.041	0.426	3.4.24.69	NA
4	0.060	1vbgA	0.249	8.35	0.036	0.368	2.7.9.1	NA
5	0.060	3iydD	0.256	9.42	0.038	0.410	2.7.7.6	418,551

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	1s3sE	0.228	5.51	0.078	0.274	0.23	ADP	complex1.pdb.gz	426,427,428
2	0.01	3cmvH	0.213	9.90	0.040	0.352	0.20	ANP	complex2.pdb.gz	417,418,419,452
3	0.01	3cf2A	0.264	6.42	0.062	0.335	0.25	ANP	complex3.pdb.gz	399,400,499,567
4	0.01	3izaA	0.353	8.13	0.090	0.503	0.20	ATP	complex4.pdb.gz	425,426,427,430,431,432,433,545,546
5	0.01	2vz9B	0.276	9.27	0.037	0.433	0.10	NAP	complex5.pdb.gz	419,420,425
6	0.01	3tiwA	0.067	5.25	0.039	0.080	0.14	III	complex6.pdb.gz	416,426,427
7	0.01	2vz9A	0.294	9.69	0.038	0.479	0.11	NAP	complex7.pdb.gz	424,425,426,552,570,610
8	0.01	3cmvE	0.245	9.68	0.050	0.400	0.18	ANP	complex8.pdb.gz	392,393,395,416,420
9	0.01	3hu1A	0.220	4.74	0.071	0.253	0.20	ATG	complex9.pdb.gz	423,425,426,427,430,431,432

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.