D-I-TASSER P09467

D-I-TASSER results for P09467

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: P09467
Protein Name: Fructose-1,6-bisphosphatase 1
Gene Name: FBP1

Input Sequence in FASTA format

>P09467 (338 residues)
MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGI
AGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDP
LDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDC
GVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAP
YGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGK
EAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	P09467-D1	1-200	200		MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKD
2	P09467-D2	201-338	138		VKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.89

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1fsaA	0.93	1.51	0.849	0.959	model1_1fsaA.pdb.gz
2	5oeyA	0.92	1.19	0.451	0.944	model1_5oeyA.pdb.gz
3	2qvrA	0.91	1.77	0.438	0.953	model1_2qvrA.pdb.gz
4	7k74A	0.90	1.58	0.377	0.941	model1_7k74A.pdb.gz
5	7ns5A	0.89	1.50	0.454	0.923	model1_7ns5A.pdb.gz
6	1d9qB	0.85	2.46	0.444	0.920	model1_1d9qB.pdb.gz
7	5iz3A	0.82	2.16	0.297	0.870	model1_5iz3A.pdb.gz
8	3uksA	0.81	2.21	0.305	0.873	model1_3uksA.pdb.gz
9	1dk4A	0.64	2.74	0.155	0.725	model1_1dk4A.pdb.gz
10	5eq7A	0.63	3.16	0.134	0.731	model1_5eq7A.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0016791	1.00	phosphatase activity
GO:0042132	0.98	fructose 1,6-bisphosphate 1-phosphatase activity
GO:1901363	0.71	heterocyclic compound binding
GO:0097159	0.71	organic cyclic compound binding
GO:0043167	0.65	ion binding
GO:0046872	0.64	metal ion binding
GO:0036094	0.60	small molecule binding
GO:0032555	0.58	purine ribonucleotide binding
GO:0032550	0.58	purine ribonucleoside binding
GO:0032559	0.57	adenyl ribonucleotide binding
GO:0048029	0.53	monosaccharide binding
GO:0016208	0.52	AMP binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0008152	0.96	metabolic process
GO:0071704	0.94	organic substance metabolic process
GO:0044238	0.93	primary metabolic process
GO:0044699	0.90	single-organism process
GO:1901576	0.89	organic substance biosynthetic process
GO:0044710	0.89	single-organism metabolic process
GO:0009987	0.88	cellular process
GO:0044723	0.86	single-organism carbohydrate metabolic process
GO:0044281	0.84	small molecule metabolic process
GO:0044237	0.83	cellular metabolic process
GO:0016051	0.81	carbohydrate biosynthetic process
GO:0006094	0.77	gluconeogenesis
GO:0050896	0.74	response to stimulus
GO:0042221	0.69	response to chemical
GO:0019637	0.59	organophosphate metabolic process
GO:0006796	0.59	phosphate-containing compound metabolic process
GO:1901135	0.58	carbohydrate derivative metabolic process
GO:0065007	0.57	biological regulation
GO:0051716	0.57	cellular response to stimulus
GO:0050789	0.57	regulation of biological process
GO:0050794	0.56	regulation of cellular process
GO:0070887	0.55	cellular response to chemical stimulus
GO:0080090	0.53	regulation of primary metabolic process
GO:0031323	0.53	regulation of cellular metabolic process
GO:0043933	0.52	macromolecular complex subunit organization
GO:0019219	0.52	regulation of nucleobase-containing compound metabolic process
GO:0009889	0.52	regulation of biosynthetic process
GO:0051289	0.51	protein homotetramerization
GO:0048523	0.51	negative regulation of cellular process
GO:0051128	0.50	regulation of cellular component organization
GO:0042325	0.50	regulation of phosphorylation
GO:0031324	0.50	negative regulation of cellular metabolic process
GO:0023057	0.50	negative regulation of signaling
GO:0016311	0.50	dephosphorylation
GO:0010648	0.50	negative regulation of cell communication
GO:0009966	0.50	regulation of signal transduction

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0044424	0.99	intracellular part
GO:0044444	0.95	cytoplasmic part
GO:0005829	0.84	cytosol
GO:0043226	0.73	organelle
GO:0043227	0.72	membrane-bounded organelle
GO:0043229	0.63	intracellular organelle
GO:0005737	0.63	cytoplasm
GO:0043231	0.61	intracellular membrane-bounded organelle
GO:0005634	0.51	nucleus

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.692	1spiA	0.854	1.83	0.436	0.902	3.1.3.11	75,99
2	0.634	2gq1A	0.865	1.69	0.441	0.902	3.1.3.11	75,99
3	0.436	1lbvA	0.657	2.48	0.154	0.731	3.1.3.25	98
4	0.376	1awbA	0.617	3.14	0.119	0.716	3.1.3.25	98
5	0.367	2p3nA	0.610	3.43	0.130	0.719	3.1.3.25	98

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.70	2vt5B	0.903	1.40	0.984	0.926	2.01	ROK	complex1.pdb.gz	19,21,22,23,25,27,28,29,30,31,32
2	0.69	1nv1A	0.959	1.32	0.903	0.979	1.78	UUU	complex2.pdb.gz	98,119,121,122,123,124,213,216,245,247,249,265,275,276,281
3	0.59	1levF	0.909	0.79	0.897	0.920	1.85	CLI	complex3.pdb.gz	17,18,21,22,27,31,114,141,161,176,178
4	0.58	1fpbA	0.907	1.20	0.888	0.923	1.91	FDP	complex4.pdb.gz	21,22,25,27,28,29,30,31,113,114,141,161,178,179
5	0.57	1nuwA	0.956	1.03	0.905	0.970	1.55	PO3	complex5.pdb.gz	69,98,119,121,122,123,124
6	0.50	1eyiA	0.960	1.32	0.903	0.979	1.30	PO4	complex6.pdb.gz	27,28,29,30,31,113,114
7	0.48	2fixL	0.899	1.29	0.965	0.923	1.77	870	complex7.pdb.gz	27,28,29,32
8	0.47	1bk40	0.906	1.14	0.895	0.923	1.89	III	complex8.pdb.gz	49,50,51,53,54,125,126,128,129,130,132,167,169,170,171,172,173,186,187,188,189,195,197,213,214,215,217,218,232,233,240,241,242,243,244,245,246,255,259
9	0.38	1eykA	0.911	1.70	0.871	0.941	1.33	PO4	complex9.pdb.gz	98,119,121,281
10	0.14	1fpiB	0.907	1.33	0.880	0.926	1.10	K	complex10.pdb.gz	119,120,121,122,124

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.