D-I-TASSER P01588

D-I-TASSER results for P01588

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: P01588
Protein Name: Erythropoietin
Gene Name: EPO

Input Sequence in FASTA format

>P01588 (193 residues)
MGVHECPAWLWLLLSLLSLPLGLPVLGAPPRLICDSRVLERYLLEAKEAENITTGCAEHC
SLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQL
HVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKL
KLYTGEACRTGDR

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	P01588-D1	1-193	193		MGVHECPAWLWLLLSLLSLPLGLPVLGAPPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.7292

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1buyA	0.83	1.16	0.982	0.860	model1_1buyA.pdb.gz
2	1v7nV	0.64	2.35	0.207	0.736	model1_1v7nV.pdb.gz
3	1rw5A	0.61	3.70	0.058	0.808	model1_1rw5A.pdb.gz
4	4mhlA	0.60	3.09	0.072	0.736	model1_4mhlA.pdb.gz
5	1cnt1	0.60	3.01	0.069	0.731	model1_1cnt1.pdb.gz
6	1f6fA	0.59	3.45	0.115	0.772	model1_1f6fA.pdb.gz
7	4dkcA	0.56	3.67	0.074	0.720	model1_4dkcA.pdb.gz
8	2q7nB	0.55	3.52	0.078	0.720	model1_2q7nB.pdb.gz
9	1t0sB	0.55	3.39	0.089	0.720	model1_1t0sB.pdb.gz
10	1xmgC	0.55	3.47	0.048	0.720	model1_1xmgC.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0005102	0.66	receptor binding
GO:0098772	0.59	molecular function regulator
GO:0005126	0.59	cytokine receptor binding
GO:0019887	0.58	protein kinase regulator activity
GO:0030295	0.57	protein kinase activator activity
GO:0005128	0.57	erythropoietin receptor binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0050896	0.96	response to stimulus
GO:0044699	0.96	single-organism process
GO:0065007	0.94	biological regulation
GO:0050789	0.94	regulation of biological process
GO:0050794	0.93	regulation of cellular process
GO:0048518	0.91	positive regulation of biological process
GO:0080090	0.90	regulation of primary metabolic process
GO:0060255	0.90	regulation of macromolecule metabolic process
GO:0048522	0.90	positive regulation of cellular process
GO:0031323	0.90	regulation of cellular metabolic process
GO:0048584	0.89	positive regulation of response to stimulus
GO:0023051	0.89	regulation of signaling
GO:0010646	0.89	regulation of cell communication
GO:0001934	0.89	positive regulation of protein phosphorylation
GO:1902533	0.88	positive regulation of intracellular signal transduction
GO:0048523	0.84	negative regulation of cellular process
GO:0042127	0.84	regulation of cell proliferation
GO:0010941	0.84	regulation of cell death
GO:0006950	0.84	response to stress
GO:0060548	0.83	negative regulation of cell death
GO:0008284	0.83	positive regulation of cell proliferation
GO:0006970	0.83	response to osmotic stress
GO:0009987	0.78	cellular process
GO:0044763	0.74	single-organism cellular process
GO:0048869	0.71	cellular developmental process
GO:0032502	0.64	developmental process
GO:0044767	0.63	single-organism developmental process
GO:0051171	0.62	regulation of nitrogen compound metabolic process
GO:0031326	0.62	regulation of cellular biosynthetic process
GO:0065008	0.61	regulation of biological quality
GO:0045935	0.61	positive regulation of nucleobase-containing compound metabolic process
GO:0031328	0.61	positive regulation of cellular biosynthetic process
GO:0010557	0.61	positive regulation of macromolecule biosynthetic process
GO:0006355	0.61	regulation of transcription, DNA-templated
GO:0065009	0.60	regulation of molecular function
GO:0051716	0.60	cellular response to stimulus
GO:0045893	0.60	positive regulation of transcription, DNA-templated
GO:0006357	0.60	regulation of transcription from RNA polymerase II promoter
GO:2001258	0.59	negative regulation of cation channel activity
GO:1902251	0.59	negative regulation of erythrocyte apoptotic process
GO:1902219	0.59	negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress
GO:0071474	0.59	cellular hyperosmotic response
GO:0046579	0.59	positive regulation of Ras protein signal transduction
GO:0045740	0.59	positive regulation of DNA replication
GO:0042523	0.59	positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0030218	0.59	erythrocyte differentiation
GO:0010523	0.59	negative regulation of calcium ion transport into cytosol
GO:0000122	0.59	negative regulation of transcription from RNA polymerase II promoter

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0005615	0.88	extracellular space
GO:0009986	0.62	cell surface
GO:0044424	0.56	intracellular part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1qleC	0.458	4.62	0.029	0.689	1.9.3.1	NA
2	0.060	2uw1B	0.506	4.33	0.041	0.710	1.14.99.6	NA
3	0.060	1fziA	0.507	4.18	0.067	0.720	1.14.13.25	NA
4	0.060	2bq1I	0.515	4.17	0.031	0.720	1.17.4.1	NA
5	0.060	3es3A	0.446	3.39	0.078	0.560	1.16.3.1	176

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.02	2inc0	0.555	3.29	0.097	0.715	0.62	III	complex1.pdb.gz	13,15,16,19,20,22,23,24,184
2	0.02	1a220	0.544	3.97	0.094	0.751	0.54	III	complex2.pdb.gz	43,44,47,74,75,78,79,173,174,177,178,180,181,184,185,188,191
3	0.01	1w68A	0.526	4.06	0.052	0.715	0.56	FEO	complex3.pdb.gz	91,94,179,183

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.