D-I-TASSER O95528

D-I-TASSER results for O95528

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: O95528
Protein Name: Solute carrier family 2, facilitated glucose transporter member 10
Gene Name: SLC2A10

Input Sequence in FASTA format

>O95528 (541 residues)
MGHSPPVLPLCASVSLLGGLTFGYELAVISGALLPLQLDFGLSCLEQEFLVGSLLLGALL
ASLVGGFLIDCYGRKQAILGSNLVLLAGSLTLGLAGSLAWLVLGRAVVGFAISLSSMACC
IYVSELVGPRQRGVLVSLYEAGITVGILLSYALNYALAGTPWGWRHMFGWATAPAVLQSL
SLLFLPAGTDETATHKDLIPLQGGEAPKLGPGRPRYSFLDLFRARDNMRGRTTVGLGLVL
FQQLTGQPNVLCYASTIFSSVGFHGGSSAVLASVGLGAVKVAATLTAMGLVDRAGRRALL
LAGCALMALSVSGIGLVSFAVPMDSGPSCLAVPNATGQTGLPGDSGLLQDSSLPPIPRTN
EDQREPILSTAKKTKPHPRSGDPSAPPRLALSSALPGPPLPARGHALLRWTALLCLMVFV
SAFSFGFGPVTWLVLSEIYPVEIRGRAFAFCNSFNWAANLFISLSFLDLIGTIGLSWTFL
LYGLTAVLGLGFIYLFVPETKGQSLAEIDQQFQKRRFTLSFGHRQNSTGIPYSRIEISAA
S

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	O95528-D1	1-214	214		MGHSPPVLPLCASVSLLGGLTFGYELAVISGALLPLQLDFGLSCLEQEFLVGSLLLGALLASLVGGFLIDCYGRKQAILGSNLVLLAGSLTLGLAGSLAWLVLGRAVVGFAISLSSMACCIYVSELVGPRQRGVLVSLYEAGITVGILLSYALNYALAGTPWGWRHMFGWATAPAVLQSLSLLFLPAGTDETATHKDLIPLQGGEAPKLGPGRP
2	O95528-D2	215-541	327		RYSFLDLFRARDNMRGRTTVGLGLVLFQQLTGQPNVLCYASTIFSSVGFHGGSSAVLASVGLGAVKVAATLTAMGLVDRAGRRALLLAGCALMALSVSGIGLVSFAVPMDSGPSCLAVPNATGQTGLPGDSGLLQDSSLPPIPRTNEDQREPILSTAKKTKPHPRSGDPSAPPRLALSSALPGPPLPARGHALLRWTALLCLMVFVSAFSFGFGPVTWLVLSEIYPVEIRGRAFAFCNSFNWAANLFISLSFLDLIGTIGLSWTFLLYGLTAVLGLGFIYLFVPETKGQSLAEIDQQFQKRRFTLSFGHRQNSTGIPYSRIEISAAS

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.87

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6h7dA	0.78	2.20	0.262	0.832	model1_6h7dA.pdb.gz
2	7crzA	0.76	2.01	0.271	0.798	model1_7crzA.pdb.gz
3	4gbyA	0.73	2.42	0.301	0.780	model1_4gbyA.pdb.gz
4	4ybqA	0.73	2.55	0.263	0.784	model1_4ybqA.pdb.gz
5	6m2lA	0.72	2.69	0.207	0.778	model1_6m2lA.pdb.gz
6	6thaA	0.71	2.39	0.270	0.760	model1_6thaA.pdb.gz
7	4ldsA	0.68	2.88	0.310	0.752	model1_4ldsA.pdb.gz
8	4j05A	0.67	2.43	0.180	0.717	model1_4j05A.pdb.gz
9	3wdoA	0.62	3.83	0.127	0.728	model1_3wdoA.pdb.gz
10	4zowA1	0.33	2.45	0.161	0.351	model1_4zowA1.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0022891	1.00	substrate-specific transmembrane transporter activity
GO:0051119	0.94	sugar transmembrane transporter activity
GO:0015075	0.92	ion transmembrane transporter activity
GO:0015291	0.91	secondary active transmembrane transporter activity
GO:0015149	0.91	hexose transmembrane transporter activity
GO:0008324	0.91	cation transmembrane transporter activity
GO:0015293	0.90	symporter activity
GO:0005355	0.90	glucose transmembrane transporter activity
GO:0005351	0.89	sugar:proton symporter activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0044699	0.99	single-organism process
GO:0006810	0.98	transport
GO:0071702	0.97	organic substance transport
GO:0044765	0.97	single-organism transport
GO:0008643	0.95	carbohydrate transport
GO:0008645	0.94	hexose transport
GO:0015758	0.93	glucose transport
GO:0055085	0.91	transmembrane transport
GO:0046323	0.90	glucose import
GO:0035428	0.88	hexose transmembrane transport

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	0.99	cell part
GO:0044425	0.98	membrane part
GO:0016021	0.97	integral component of membrane
GO:0005887	0.94	integral component of plasma membrane
GO:0016020	0.50	membrane

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	3eqlM	0.330	7.56	0.058	0.534	2.7.7.6	86,112
2	0.060	1h16A	0.327	6.87	0.042	0.497	2.3.1.54	NA
3	0.060	2vz8B	0.321	8.16	0.052	0.558	2.3.1.85	NA
4	0.060	2jfdA	0.217	7.23	0.059	0.344	2.3.1.85	112
5	0.060	2ow6A	0.337	7.57	0.041	0.549	3.2.1.114	70

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.02	2y5yA	0.563	4.18	0.132	0.684	0.48	TGA	complex1.pdb.gz	30,105,109
2	0.01	1hnz4	0.064	4.21	0.000	0.078	0.54	III	complex2.pdb.gz	21,22,53,54,55,57,58,59
3	0.01	2is1B	0.338	7.38	0.041	0.540	0.44	QNA	complex3.pdb.gz	21,23,27,29,30
4	0.01	1qhhA	0.153	5.20	0.048	0.202	0.44	ATP	complex4.pdb.gz	21,80,82,84,85
5	0.01	1wcm7	0.076	3.41	0.031	0.087	0.40	III	complex5.pdb.gz	25,26,48,49,51,52,59,63,66,67
6	0.01	2v469	0.087	3.61	0.016	0.102	0.42	III	complex6.pdb.gz	15,20,77,88,90,91,92,93
7	0.01	3i4mB	0.336	7.47	0.047	0.542	0.42	QNA	complex7.pdb.gz	65,66,69
8	0.01	1siwA	0.322	8.05	0.038	0.549	0.44	SF4	complex8.pdb.gz	52,53,106,109
9	0.01	2yu91	0.046	3.72	0.029	0.054	0.43	III	complex9.pdb.gz	17,18,21,22,26,27,28
10	0.01	2ja58	0.086	4.95	0.061	0.111	0.47	III	complex10.pdb.gz	23,26,27,30,32

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.