D-I-TASSER O95214

D-I-TASSER results for O95214

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: O95214
Protein Name: Leptin receptor overlapping transcript-like 1
Gene Name: LEPROTL1

Input Sequence in FASTA format

>O95214 (131 residues)
MAGIKALISLSFGGAIGLMFLMLGCALPIYNKYWPLFVLFFYILSPIPYCIARRLVDDTD
AMSNACKELAIFLTTGIVVSAFGLPIVFARAHLIEWGACALVLTGNTVIFATILGFFLVF
GSNDDFSWQQW

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	O95214-D1	1-131	131		MAGIKALISLSFGGAIGLMFLMLGCALPIYNKYWPLFVLFFYILSPIPYCIARRLVDDTDAMSNACKELAIFLTTGIVVSAFGLPIVFARAHLIEWGACALVLTGNTVIFATILGFFLVFGSNDDFSWQQW

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.7454

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5mkkB1	0.71	2.72	0.144	0.908	model1_5mkkB1.pdb.gz
2	5mkkA	0.71	2.61	0.120	0.908	model1_5mkkA.pdb.gz
3	6ajfA3	0.66	3.96	0.092	0.977	model1_6ajfA3.pdb.gz
4	5zleA	0.66	3.73	0.085	0.962	model1_5zleA.pdb.gz
5	5uj9A	0.66	3.07	0.101	0.901	model1_5uj9A.pdb.gz
6	6jbjA	0.65	3.75	0.077	0.954	model1_6jbjA.pdb.gz
7	4wd7A	0.65	3.10	0.069	0.863	model1_4wd7A.pdb.gz
8	4mrnA	0.65	3.31	0.117	0.901	model1_4mrnA.pdb.gz
9	5u1dB	0.65	3.47	0.046	0.916	model1_5u1dB.pdb.gz
10	5u71A1	0.65	3.36	0.084	0.931	model1_5u71A1.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0005198	0.74	structural molecule activity
GO:0003735	0.51	structural constituent of ribosome

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.77	biological regulation
GO:0009987	0.76	cellular process
GO:0046426	0.75	negative regulation of JAK-STAT cascade
GO:2000009	0.58	negative regulation of protein localization to cell surface
GO:1903955	0.58	positive regulation of protein targeting to mitochondrion
GO:0060400	0.58	negative regulation of growth hormone receptor signaling pathway
GO:0006810	0.52	transport

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044444	1.00	cytoplasmic part
GO:0043231	1.00	intracellular membrane-bounded organelle
GO:0044446	0.88	intracellular organelle part
GO:0005773	0.77	vacuole
GO:0016020	0.71	membrane
GO:0032991	0.66	macromolecular complex
GO:0005794	0.66	Golgi apparatus
GO:0044425	0.65	membrane part
GO:0005768	0.62	endosome
GO:0016021	0.55	integral component of membrane

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2vo4B	0.528	4.04	0.088	0.824	2.5.1.18	NA
2	0.060	2j1pB	0.498	3.86	0.046	0.733	2.5.1.1 2.5.1.10 2.5.1.29	116
3	0.060	1xvgC	0.525	3.98	0.033	0.817	1.14.13.25	12,27,104
4	0.060	3g61A	0.609	3.80	0.084	0.939	3.6.3.44	NA
5	0.060	1xmeA	0.530	4.37	0.055	0.855	1.9.3.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.07	2yl4A	0.643	3.16	0.128	0.901	0.73	CDL	complex1.pdb.gz	19,23,43,47,84
2	0.06	2eimC	0.622	3.87	0.087	0.947	0.94	CDL	complex2.pdb.gz	14,18,21,22,102,105,106
3	0.03	2yl4A	0.643	3.16	0.128	0.901	0.80	14Y	complex3.pdb.gz	18,21,22,25
4	0.02	2einC	0.623	3.81	0.094	0.939	0.79	CDL	complex4.pdb.gz	14,18,21
5	0.02	2eikC	0.623	3.80	0.094	0.939	0.78	CD	complex5.pdb.gz	17,21,102,106

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.