D-I-TASSER O94919

D-I-TASSER results for O94919

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: O94919
Protein Name: Endonuclease domain-containing 1 protein
Gene Name: ENDOD1

Input Sequence in FASTA format

>O94919 (500 residues)
MGTARWLALGSLFALAGLLEGRLVGEEEAGFGECDKFFYAGTPPAGLAADSHVKICQRAE
GAERFATLYSTRDRIPVYSAFRAPRPAPGGAEQRWLVEPQIDDPNSNLEEAINEAEAITS
VNSLGSKQALNTDYLDSDYQRGQLYPFSLSSDVQVATFTLTNSAPMTQSFQERWYVNLHS
LMDRALTPQCGSGEDLYILTGTVPSDYRVKDKVAVPEFVWLAACCAVPGGGWAMGFVKHT
RDSDIIEDVMVKDLQKLLPFNPQLFQNNCGETEQDTEKMKKILEVVNQIQDEERMVQSQK
SSSPLSSTRSKRSTLLPPEASEGSSSFLGKLMGFIATPFIKLFQLIYYLVVAILKNIVYF
LWCVTKQVINGIESCLYRLGSATISYFMAIGEELVSIPWKVLKVVAKVIRALLRILCCLL
KAICRVLSIPVRVLVDVATFPVYTMGAIPIVCKDIALGLGGTVSLLFDTAFGTLGGLFQV
VFSVCKRIGYKVTFDNSGEL

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	O94919-D1	1-407	407		MGTARWLALGSLFALAGLLEGRLVGEEEAGFGECDKFFYAGTPPAGLAADSHVKICQRAEGAERFATLYSTRDRIPVYSAFRAPRPAPGGAEQRWLVEPQIDDPNSNLEEAINEAEAITSVNSLGSKQALNTDYLDSDYQRGQLYPFSLSSDVQVATFTLTNSAPMTQSFQERWYVNLHSLMDRALTPQCGSGEDLYILTGTVPSDYRVKDKVAVPEFVWLAACCAVPGGGWAMGFVKHTRDSDIIEDVMVKDLQKLLPFNPQLFQNNCGETEQDTEKMKKILEVVNQIQDEERMVQSQKSSSPLSSTRSKRSTLLPPEASEGSSSFLGKLMGFIATPFIKLFQLIYYLVVAILKNIVYFLWCVTKQVINGIESCLYRLGSATISYFMAIGEELVSIPWKVLKVVAK
2	O94919-D2	408-500	93		VIRALLRILCCLLKAICRVLSIPVRVLVDVATFPVYTMGAIPIVCKDIALGLGGTVSLLFDTAFGTLGGLFQVVFSVCKRIGYKVTFDNSGEL

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.58

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	4a1nA	0.44	3.07	0.152	0.484	model1_4a1nA.pdb.gz
2	3ismB	0.41	2.05	0.170	0.432	model1_3ismB.pdb.gz
3	5gz4A	0.41	3.71	0.162	0.464	model1_5gz4A.pdb.gz
4	2xr9A2	0.41	2.99	0.163	0.450	model1_2xr9A2.pdb.gz
5	2o3bA	0.41	2.30	0.194	0.432	model1_2o3bA.pdb.gz
6	4b56A	0.40	4.00	0.155	0.474	model1_4b56A.pdb.gz
7	1ql0A	0.40	2.71	0.132	0.438	model1_1ql0A.pdb.gz
8	3s5bA	0.40	2.10	0.179	0.422	model1_3s5bA.pdb.gz
9	1p49A	0.27	7.91	0.049	0.470	model1_1p49A.pdb.gz
10	1n2lA	0.26	7.89	0.038	0.434	model1_1n2lA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.91	catalytic activity
GO:0016787	0.85	hydrolase activity
GO:0016788	0.84	hydrolase activity, acting on ester bonds
GO:0004518	0.77	nuclease activity
GO:1901363	0.66	heterocyclic compound binding
GO:0097159	0.66	organic cyclic compound binding
GO:0004536	0.60	deoxyribonuclease activity
GO:0004540	0.57	ribonuclease activity
GO:0046872	0.55	metal ion binding
GO:0004519	0.53	endonuclease activity
GO:0003676	0.53	nucleic acid binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.95	cellular process
GO:0008152	0.89	metabolic process
GO:0071704	0.88	organic substance metabolic process
GO:0044238	0.88	primary metabolic process
GO:0044237	0.88	cellular metabolic process
GO:0044260	0.86	cellular macromolecule metabolic process
GO:0006807	0.86	nitrogen compound metabolic process
GO:0006139	0.83	nucleobase-containing compound metabolic process
GO:0090304	0.82	nucleic acid metabolic process
GO:0009056	0.81	catabolic process
GO:1901575	0.79	organic substance catabolic process
GO:0044248	0.79	cellular catabolic process
GO:0034655	0.78	nucleobase-containing compound catabolic process
GO:0006259	0.78	DNA metabolic process
GO:0000737	0.76	DNA catabolic process, endonucleolytic
GO:0044699	0.66	single-organism process
GO:0044763	0.58	single-organism cellular process
GO:0006309	0.58	apoptotic DNA fragmentation
GO:0016043	0.56	cellular component organization
GO:0016070	0.51	RNA metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0070062	1.00	extracellular exosome
GO:0016020	1.00	membrane
GO:0044464	0.90	cell part
GO:0044424	0.86	intracellular part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	3ecqB	0.365	7.54	0.029	0.610	3.2.1.97	NA
2	0.060	1clqA	0.243	7.25	0.029	0.380	2.7.7.7	NA
3	0.060	1o98A	0.348	7.08	0.091	0.548	5.4.2.1	NA
4	0.060	3gm8A	0.350	7.24	0.039	0.564	3.2.1.-	NA
5	0.060	2ifyA	0.271	7.53	0.029	0.448	5.4.2.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	2gbiA	0.345	7.25	0.045	0.564	0.16	XIH	complex1.pdb.gz	145,199,222,234,264
2	0.01	2bgn0	0.356	7.51	0.030	0.586	0.24	III	complex2.pdb.gz	179,180,181,183,184
3	0.01	3cccB	0.319	7.30	0.028	0.520	0.16	7AC	complex3.pdb.gz	141,142,166,214,215
4	0.01	1u8eB	0.347	7.16	0.027	0.556	0.37	UUU	complex4.pdb.gz	162,164,240
5	0.01	2oncB	0.359	7.48	0.038	0.590	0.23	SY1	complex5.pdb.gz	141,199,201,202
6	0.01	1g8t0	0.403	2.71	0.132	0.438	0.13	III	complex6.pdb.gz	128,200,201,202,262
7	0.01	3h0cA	0.353	7.56	0.040	0.588	0.11	PS4	complex7.pdb.gz	145,224,234,248
8	0.01	2h642	0.105	4.98	0.074	0.138	0.25	III	complex8.pdb.gz	213,215,217
9	0.01	3noxA	0.357	7.50	0.028	0.588	0.14	6A5	complex9.pdb.gz	145,221,264
10	0.01	3c43A	0.324	7.38	0.044	0.530	0.12	315	complex10.pdb.gz	221,222,265

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.