D-I-TASSER O75475

D-I-TASSER results for O75475

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: O75475
Protein Name: PC4 and SFRS1-interacting protein
Gene Name: PSIP1

Input Sequence in FASTA format

>O75475 (530 residues)
MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFP
YSENKEKYGKPNKRKGFNEGLWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKE
DTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKR
GRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKE
EDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRR
NMLKGQHEKEAADRKRKQEEQMETEQQNKDEGKKPEVKKVEKKRETSMDSRLQRIHAEIK
NSLKIDNLDVNRCIEALDELASLQVTMQQAQKHTEMITTLKKIRRFKVSQVIMEKSTMLY
NKFKNMFLVGEGDSVITQVLNKSLAEQRQHEEANKTKDQGKKGPNKKLEKEQTGSKTLNG
GSDAQDGNQPQHNGESNEDSKDNHEASTKKKPSSEERETEISLKDSTLDN

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	O75475-D1	1-530	530		MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEQQNKDEGKKPEVKKVEKKRETSMDSRLQRIHAEIKNSLKIDNLDVNRCIEALDELASLQVTMQQAQKHTEMITTLKKIRRFKVSQVIMEKSTMLYNKFKNMFLVGEGDSVITQVLNKSLAEQRQHEEANKTKDQGKKGPNKKLEKEQTGSKTLNGGSDAQDGNQPQHNGESNEDSKDNHEASTKKKPSSEERETEISLKDSTLDN

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.3578

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	4cr2Z	0.95	1.67	0.060	0.983	model1_4cr2Z.pdb.gz
2	5l4kZ	0.86	3.38	0.067	0.972	model1_5l4kZ.pdb.gz
3	5mpbZ	0.85	3.37	0.050	0.970	model1_5mpbZ.pdb.gz
4	5mpbN	0.82	3.86	0.045	0.968	model1_5mpbN.pdb.gz
5	5l4kN	0.81	3.93	0.045	0.966	model1_5l4kN.pdb.gz
6	4ui9A	0.54	3.88	0.044	0.640	model1_4ui9A.pdb.gz
7	2z6gA	0.30	6.82	0.021	0.466	model1_2z6gA.pdb.gz
8	2f02A	0.24	6.51	0.073	0.351	model1_2f02A.pdb.gz
9	4s3kA	0.24	7.22	0.042	0.379	model1_4s3kA.pdb.gz
10	4zzpA	0.22	7.57	0.047	0.366	model1_4zzpA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003676	0.93	nucleic acid binding
GO:0003677	0.88	DNA binding
GO:0044877	0.85	macromolecular complex binding
GO:0003682	0.84	chromatin binding
GO:0003712	0.83	transcription cofactor activity
GO:0043566	0.82	structure-specific DNA binding
GO:0008134	0.80	transcription factor binding
GO:0001191	0.80	transcriptional repressor activity, RNA polymerase II transcription factor binding
GO:0001104	0.80	RNA polymerase II transcription cofactor activity
GO:0097100	0.78	supercoiled DNA binding
GO:0033613	0.78	activating transcription factor binding
GO:0001105	0.78	RNA polymerase II transcription coactivator activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0050794	0.99	regulation of cellular process
GO:0009987	0.95	cellular process
GO:0080090	0.92	regulation of primary metabolic process
GO:0060255	0.92	regulation of macromolecule metabolic process
GO:0051171	0.92	regulation of nitrogen compound metabolic process
GO:0031323	0.92	regulation of cellular metabolic process
GO:0019219	0.91	regulation of nucleobase-containing compound metabolic process
GO:0016043	0.89	cellular component organization
GO:2001141	0.88	regulation of RNA biosynthetic process
GO:2000112	0.88	regulation of cellular macromolecule biosynthetic process
GO:0010468	0.88	regulation of gene expression
GO:0048522	0.87	positive regulation of cellular process
GO:0006355	0.87	regulation of transcription, DNA-templated
GO:0050896	0.86	response to stimulus
GO:0043933	0.85	macromolecular complex subunit organization
GO:0006950	0.85	response to stress
GO:0034622	0.84	cellular macromolecular complex assembly
GO:0009628	0.84	response to abiotic stimulus
GO:0006357	0.84	regulation of transcription from RNA polymerase II promoter
GO:0031325	0.83	positive regulation of cellular metabolic process
GO:0010604	0.83	positive regulation of macromolecule metabolic process
GO:0045893	0.82	positive regulation of transcription, DNA-templated
GO:0045944	0.81	positive regulation of transcription from RNA polymerase II promoter
GO:0009408	0.80	response to heat
GO:0006979	0.80	response to oxidative stress
GO:0000395	0.80	mRNA 5'-splice site recognition

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0044446	0.93	intracellular organelle part
GO:0044428	0.92	nuclear part
GO:0032991	0.87	macromolecular complex
GO:0044427	0.79	chromosomal part
GO:0035327	0.78	transcriptionally active chromatin
GO:0005720	0.78	nuclear heterochromatin
GO:0043226	0.68	organelle
GO:0043229	0.67	intracellular organelle
GO:0043227	0.62	membrane-bounded organelle
GO:0043231	0.60	intracellular membrane-bounded organelle
GO:0005634	0.57	nucleus

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1nxcA	0.368	6.16	0.055	0.534	3.2.1.113	NA
2	0.060	2a4zA	0.343	7.00	0.033	0.530	2.7.1.153	NA
3	0.060	2rd0A	0.392	8.09	0.028	0.685	2.7.1.153	NA
4	0.060	1fo2A	0.355	6.30	0.059	0.523	3.2.1.113	43
5	0.060	2vdcF	0.346	8.06	0.049	0.596	1.4.1.13	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	3q22A	0.365	7.76	0.048	0.611	0.18	GTP	complex1.pdb.gz	7,19,25,26,27
2	0.01	3b2pA	0.381	5.78	0.052	0.524	0.11	ARG	complex2.pdb.gz	59,60,63
3	0.01	3eqoA	0.382	7.41	0.044	0.611	0.14	LGC	complex3.pdb.gz	8,43,44
4	0.01	3hazA	0.374	6.99	0.049	0.574	0.10	NAD	complex4.pdb.gz	3,4,5
5	0.01	2dqmA	0.375	6.31	0.028	0.534	0.30	BES	complex5.pdb.gz	17,18,19,20,55,56,59
6	0.01	3pffA	0.370	7.24	0.062	0.598	0.17	ADP	complex6.pdb.gz	5,44,52,56

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.