D-I-TASSER O60235

D-I-TASSER results for O60235

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: O60235
Protein Name: Transmembrane protease serine 11D
Gene Name: TMPRSS11D

Input Sequence in FASTA format

>O60235 (418 residues)
MYRPARVTSTSRFLNPYVVCFIVVAGVVILAVTIALLVYFLAFDQKSYFYRSSFQLLNVE
YNSQLNSPATQEYRTLSGRIESLITKTFKESNLRNQFIRAHVAKLRQDGSGVRADVVMKF
QFTRNNNGASMKSRIESVLRQMLNNSGNLEINPSTEITSLTDQAAANWLINECGAGPDLI
TLSEQRILGGTEAEEGSWPWQVSLRLNNAHHCGGSLINNMWILTAAHCFRSNSNPRDWIA
TSGISTTFPKLRMRVRNILIHNNYKSATHENDIALVRLENSVTFTKDIHSVCLPAATQNI
PPGSTAYVTGWGAQEYAGHTVPELRQGQVRIISNDVCNAPHSYNGAILSGMLCAGVPQGG
VDACQGDSGGPLVQEDSRRLWFIVGIVSWGDQCGLPDKPGVYTRVTAYLDWIRQQTGI

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	O60235-D1	1-159	159		MYRPARVTSTSRFLNPYVVCFIVVAGVVILAVTIALLVYFLAFDQKSYFYRSSFQLLNVEYNSQLNSPATQEYRTLSGRIESLITKTFKESNLRNQFIRAHVAKLRQDGSGVRADVVMKFQFTRNNNGASMKSRIESVLRQMLNNSGNLEINPSTEITS
2	O60235-D2	160-418	259		LTDQAAANWLINECGAGPDLITLSEQRILGGTEAEEGSWPWQVSLRLNNAHHCGGSLINNMWILTAAHCFRSNSNPRDWIATSGISTTFPKLRMRVRNILIHNNYKSATHENDIALVRLENSVTFTKDIHSVCLPAATQNIPPGSTAYVTGWGAQEYAGHTVPELRQGQVRIISNDVCNAPHSYNGAILSGMLCAGVPQGGVDACQGDSGGPLVQEDSRRLWFIVGIVSWGDQCGLPDKPGVYTRVTAYLDWIRQQTGI

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.76

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1z8gA	0.62	3.25	0.332	0.679	model1_1z8gA.pdb.gz
2	2xxlA	0.61	4.07	0.263	0.720	model1_2xxlA.pdb.gz
3	7meqA	0.61	3.12	0.344	0.663	model1_7meqA.pdb.gz
4	4hzhB	0.60	3.82	0.253	0.689	model1_4hzhB.pdb.gz
5	2f83A	0.58	3.33	0.263	0.660	model1_2f83A.pdb.gz
6	1jwtA	0.57	1.81	0.337	0.596	model1_1jwtA.pdb.gz
7	1a5iA	0.56	1.75	0.336	0.584	model1_1a5iA.pdb.gz
8	2vntC	0.56	1.44	0.357	0.574	model1_2vntC.pdb.gz
9	1bmlA	0.56	1.93	0.352	0.579	model1_1bmlA.pdb.gz
10	1yc0A	0.55	1.33	0.329	0.565	model1_1yc0A.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0008233	0.98	peptidase activity
GO:0070011	0.96	peptidase activity, acting on L-amino acid peptides
GO:0008236	0.92	serine-type peptidase activity
GO:0004175	0.83	endopeptidase activity
GO:0004252	0.79	serine-type endopeptidase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0008152	0.83	metabolic process
GO:0071704	0.82	organic substance metabolic process
GO:0044238	0.81	primary metabolic process
GO:0043170	0.79	macromolecule metabolic process
GO:0019538	0.76	protein metabolic process
GO:0006508	0.71	proteolysis
GO:0044699	0.55	single-organism process
GO:0065007	0.53	biological regulation

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	0.84	cell part
GO:0044424	0.69	intracellular part
GO:0044421	0.53	extracellular region part
GO:0043226	0.46	organelle
GO:0005615	0.46	extracellular space
GO:0043227	0.43	membrane-bounded organelle
GO:0043229	0.36	intracellular organelle
GO:0044444	0.33	cytoplasmic part
GO:0043231	0.33	intracellular membrane-bounded organelle
GO:0005737	0.30	cytoplasm

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.412	2qxhA	0.508	1.30	0.390	0.521	3.4.21.117	190,212,227,352,355,363,366,387
2	0.405	1fn8A	0.515	1.27	0.376	0.529	3.4.21.4	227
3	0.368	1tgsZ	0.510	1.42	0.365	0.524	3.4.21.4	364,366,367
4	0.218	1eq9A	0.514	1.41	0.330	0.529	3.4.21.1	227
5	0.218	1eawA	0.541	1.03	0.409	0.550	3.4.21.109 3.4.21.-	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.51	1z8gA	0.617	3.25	0.332	0.679	1.34	III	complex1.pdb.gz	227,269,316,346,362,363,364,365,366,368,388,389,390,391,392,393,400
2	0.51	1uvsH	0.522	1.32	0.375	0.536	1.10	I11	complex2.pdb.gz	269,362,363,364,365,387,388,389,390,392,393
3	0.49	1a3bH	0.534	1.43	0.361	0.550	1.01	T29	complex3.pdb.gz	227,362,363,366,368,388,389,390,392,400
4	0.29	3da9B	0.533	1.34	0.367	0.548	0.89	44U	complex4.pdb.gz	227,364,368,387,388,389,390,391
5	0.29	3p17H	0.533	1.34	0.367	0.548	0.89	99P	complex5.pdb.gz	365,368,388,389,390,392
6	0.27	1umaH	0.535	1.34	0.361	0.550	0.97	IN2	complex6.pdb.gz	227,362,363,364,365,368,392
7	0.20	3p70B	0.533	1.37	0.362	0.548	1.74	NA	complex7.pdb.gz	214,215,223,224,369,370,371,383,386
8	0.19	1sb1H	0.531	1.32	0.364	0.545	1.57	NA	complex8.pdb.gz	201,213,309,310,367,370
9	0.17	1tmbH	0.533	1.36	0.367	0.548	1.26	III	complex9.pdb.gz	194,195,196,197,199,200,217,218,219,284,285,286,289,290,291,292,293,304,305,306,307,328,374,375,377,380,381
10	0.10	4htc0	0.536	1.29	0.361	0.550	1.12	III	complex10.pdb.gz	209,210,227,239,241,251,269,344,365,368,388,389,390,391,392,393,395,398

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.