D-I-TASSER O14681

D-I-TASSER results for O14681

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: O14681
Protein Name: Etoposide-induced protein 2.4 homolog
Gene Name: EI24

Input Sequence in FASTA format

>O14681 (340 residues)
MADSVKTFLQDLARGIKDSIWGICTISKLDARIQQKREEQRRRRASSVLAQRRAQSIERK
QESEPRIVSRIFQCCAWNGGVFWFSLLLFYRVFIPVLQSVTARIIGDPSLHGDVWSWLEF
FLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAFEVSGRKPHPFPSVSKIIADMLFNLLL
QALFLIQGMFVSLFPIHLVGQLVSLLHMSLLYSLYCFEYRWFNKGIEMHQRLSNIERNWP
YYFGFGLPLAFLTAMQSSYIISGCLFSILFPLFIISANEAKTPGKAYLFQLRLFSLVVFL
SNRLFHKTVYLQSALSSSTSAEKFPSPHPSPAKLKATAGH

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	O14681-D1	1-61	61		MADSVKTFLQDLARGIKDSIWGICTISKLDARIQQKREEQRRRRASSVLAQRRAQSIERKQ
2	O14681-D2	62-340	279		ESEPRIVSRIFQCCAWNGGVFWFSLLLFYRVFIPVLQSVTARIIGDPSLHGDVWSWLEFFLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAFEVSGRKPHPFPSVSKIIADMLFNLLLQALFLIQGMFVSLFPIHLVGQLVSLLHMSLLYSLYCFEYRWFNKGIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIISGCLFSILFPLFIISANEAKTPGKAYLFQLRLFSLVVFLSNRLFHKTVYLQSALSSSTSAEKFPSPHPSPAKLKATAGH

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.53

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6d9zF	0.58	2.47	0.122	0.635	model1_6d9zF.pdb.gz
2	3tx3A	0.50	3.60	0.114	0.600	model1_3tx3A.pdb.gz
3	6d79A	0.49	2.58	0.113	0.541	model1_6d79A.pdb.gz
4	7jsnA	0.44	6.25	0.053	0.703	model1_7jsnA.pdb.gz
5	6mzbB	0.44	6.27	0.031	0.700	model1_6mzbB.pdb.gz
6	7cunG	0.40	5.91	0.050	0.626	model1_7cunG.pdb.gz
7	5x0mA	0.40	6.33	0.053	0.641	model1_5x0mA.pdb.gz
8	3mk4A	0.40	6.02	0.093	0.624	model1_3mk4A.pdb.gz
9	1f31A	0.39	5.58	0.042	0.582	model1_1f31A.pdb.gz
10	1olpA	0.39	5.41	0.045	0.577	model1_1olpA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:1901363	0.28	heterocyclic compound binding
GO:0097159	0.28	organic cyclic compound binding
GO:0046983	0.20	protein dimerization activity
GO:0003824	0.17	catalytic activity
GO:0003676	0.16	nucleic acid binding
GO:0043167	0.15	ion binding
GO:0046872	0.14	metal ion binding
GO:0022891	0.14	substrate-specific transmembrane transporter activity
GO:0015171	0.12	amino acid transmembrane transporter activity
GO:0008289	0.12	lipid binding
GO:0000166	0.12	nucleotide binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.97	biological regulation
GO:0050789	0.96	regulation of biological process
GO:0009987	0.92	cellular process
GO:0050794	0.86	regulation of cellular process
GO:0048523	0.81	negative regulation of cellular process
GO:0051128	0.80	regulation of cellular component organization
GO:0030308	0.79	negative regulation of cell growth
GO:0048518	0.58	positive regulation of biological process
GO:0048583	0.57	regulation of response to stimulus
GO:0044699	0.57	single-organism process
GO:0048584	0.56	positive regulation of response to stimulus
GO:0044763	0.50	single-organism cellular process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0005783	1.00	endoplasmic reticulum
GO:0005794	0.90	Golgi apparatus
GO:0005737	0.90	cytoplasm
GO:0016020	0.60	membrane

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1mc0A	0.220	6.57	0.074	0.373	3.1.4.17	NA
2	0.060	1n1zA	0.362	5.74	0.028	0.535	5.5.1.8	NA
3	0.060	1z0hB	0.238	7.34	0.045	0.438	3.4.24.69	NA
4	0.060	1olpA	0.390	5.41	0.045	0.577	3.1.4.3	NA
5	0.060	1z8lA	0.376	6.65	0.063	0.635	3.4.17.21	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	3cx9A	0.382	6.15	0.025	0.597	0.27	MYR	complex1.pdb.gz	151,245,246,274,277
2	0.01	2ydfB	0.382	5.72	0.066	0.582	0.20	IO3	complex2.pdb.gz	150,222,247
3	0.01	2xvqA	0.384	5.87	0.065	0.594	0.18	9DS	complex3.pdb.gz	214,215,218,219
4	0.01	2vufA	0.384	5.94	0.061	0.600	0.31	FUA	complex4.pdb.gz	139,142,143,176,224
5	0.01	3lu7B	0.384	5.94	0.079	0.597	0.22	UUU	complex5.pdb.gz	177,180,184,185,188,189,215,246
6	0.01	2vueB	0.382	5.82	0.074	0.591	0.26	BLA	complex6.pdb.gz	139,143,165,173,177,224
7	0.01	2xw0A	0.384	5.89	0.065	0.594	0.17	9NF	complex7.pdb.gz	178,214,247
8	0.01	1n5uA	0.385	5.87	0.034	0.585	0.14	HEM	complex8.pdb.gz	173,174,177,217,221,225
9	0.01	2xw1B	0.381	5.79	0.070	0.585	0.20	9NV	complex9.pdb.gz	150,222,248
10	0.01	1g9aA	0.393	5.60	0.042	0.582	0.17	BAB	complex10.pdb.gz	169,170,171,218,220,221,222

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.