D-I-TASSER results for F8VTS6

Summary of the Protein

Uniprot ID: F8VTS6


Protein Name: Ret finger protein-like 4A-like protein 1


Gene Name: RFPL4AL1

Input Sequence in FASTA format

>F8VTS6 (287 residues)
MAEHFKQIIRCPVCLKDLEEAVQLKCGYACCLQCLNSLQKEPDGEGLLCRFCSVVSQKDD
IKPKYKLRALVSIIKELEPKLKSVLTMNPRMRKFQVDMTFDVDTANNYLIISEDLRSFRS
GDLSQNRKEQAERFDTALCVLGTPRFTSGRHYWEVDVGTSQVWDVGVCKESVNRQGKIEL
SSEHGFLTVGCREGKVFAASTVPMTPLWVSPQLHRVGIFLDVGMRSIAFYNVSDGCHINT
FIEIPVCEPWRPFFAHKRGSQDDQSILSICSVINPSTASAPVSSEGK

Predicted Contact and Distance Map Used in D-I-TASSER simulation

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Sequence
1	F8VTS6-D1	1-87	87	MAEHFKQIIRCPVCLKDLEEAVQLKCGYACCLQCLNSLQKEPDGEGLLCRFCSVVSQKDDIKPKYKLRALVSIIKELEPKLKSVLTM
2	F8VTS6-D2	88-287	200	NPRMRKFQVDMTFDVDTANNYLIISEDLRSFRSGDLSQNRKEQAERFDTALCVLGTPRFTSGRHYWEVDVGTSQVWDVGVCKESVNRQGKIELSSEHGFLTVGCREGKVFAASTVPMTPLWVSPQLHRVGIFLDVGMRSIAFYNVSDGCHINTFIEIPVCEPWRPFFAHKRGSQDDQSILSICSVINPSTASAPVSSEGK

Top 1 final models from D-I-TASSER

Rank^a	Estimated TM-score^b
1	0.8


(a) D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.
(b) The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)


Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.
1	4cg4A	0.67	3.16	0.291	0.739
2	2fbeC	0.65	1.50	0.934	0.683
3	2wl1A	0.63	1.22	0.368	0.648
4	4n7iA	0.61	1.79	0.358	0.648
5	2iwgB	0.61	0.97	0.425	0.624
6	3kb5A	0.61	1.95	0.270	0.645
7	4b8eA	0.59	1.84	0.241	0.624
8	4wvmA3	0.57	2.16	0.245	0.620
9	2lm3A	0.56	2.28	0.264	0.603
10	4ehoA	0.32	7.18	0.029	0.606



(a) Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b) Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c) RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d) IDEN^a is the percentage sequence identity in the structurally aligned region.
(e) Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)


GO term	Cscore^GO	Name
GO:0003824	0.83	catalytic activity
GO:0019787	0.80	ubiquitin-like protein transferase activity
GO:0004842	0.79	ubiquitin-protein transferase activity
GO:0046872	0.73	metal ion binding
GO:0008270	0.72	zinc ion binding
GO:0019899	0.51	enzyme binding






(a) Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

Biological Process (BP)


GO term	Cscore^GO	Name
GO:0065007	0.93	biological regulation
GO:0050789	0.92	regulation of biological process
GO:0009987	0.92	cellular process
GO:0050794	0.89	regulation of cellular process
GO:0071704	0.85	organic substance metabolic process
GO:0044238	0.84	primary metabolic process
GO:0044237	0.83	cellular metabolic process
GO:0043170	0.82	macromolecule metabolic process
GO:0044260	0.81	cellular macromolecule metabolic process
GO:0019538	0.79	protein metabolic process
GO:0044267	0.78	cellular protein metabolic process
GO:0048518	0.77	positive regulation of biological process
GO:0006464	0.77	cellular protein modification process
GO:0048583	0.76	regulation of response to stimulus
GO:0019222	0.76	regulation of metabolic process
GO:0060255	0.75	regulation of macromolecule metabolic process
GO:0031323	0.74	regulation of cellular metabolic process
GO:0080090	0.73	regulation of primary metabolic process
GO:0044699	0.73	single-organism process
GO:0032446	0.73	protein modification by small protein conjugation
GO:0050896	0.72	response to stimulus
GO:0048522	0.72	positive regulation of cellular process
GO:0048519	0.69	negative regulation of biological process
GO:0048523	0.68	negative regulation of cellular process
GO:0009966	0.68	regulation of signal transduction
GO:0044763	0.67	single-organism cellular process
GO:0016043	0.66	cellular component organization
GO:0006950	0.66	response to stress
GO:0043933	0.62	macromolecular complex subunit organization
GO:0002376	0.61	immune system process
GO:0010468	0.59	regulation of gene expression
GO:0065009	0.58	regulation of molecular function
GO:0048584	0.58	positive regulation of response to stimulus
GO:0022607	0.58	cellular component assembly
GO:0044093	0.57	positive regulation of molecular function
GO:2001141	0.56	regulation of RNA biosynthetic process
GO:0071822	0.56	protein complex subunit organization
GO:0032268	0.56	regulation of cellular protein metabolic process
GO:0032502	0.55	developmental process
GO:0006461	0.55	protein complex assembly
GO:0006355	0.54	regulation of transcription, DNA-templated
GO:0010604	0.53	positive regulation of macromolecule metabolic process
GO:0048856	0.52	anatomical structure development
GO:0031325	0.52	positive regulation of cellular metabolic process
GO:0016567	0.52	protein ubiquitination
GO:0002682	0.52	regulation of immune system process
GO:1901575	0.50	organic substance catabolic process
GO:0051259	0.50	protein oligomerization
GO:0050776	0.50	regulation of immune response
GO:0032501	0.50	multicellular organismal process






(a) Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

Cellular Component (CC)


GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0044424	0.99	intracellular part
GO:0043226	0.88	organelle
GO:0043229	0.82	intracellular organelle
GO:0044444	0.77	cytoplasmic part
GO:0043227	0.77	membrane-bounded organelle
GO:0043231	0.67	intracellular membrane-bounded organelle
GO:0005737	0.65	cytoplasm
GO:0005634	0.60	nucleus
GO:0032991	0.59	macromolecular complex
GO:0044422	0.58	organelle part
GO:0044446	0.57	intracellular organelle part
GO:0043232	0.51	intracellular non-membrane-bounded organelle






(a) Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB


Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1fo4A	0.397	6.63	0.036	0.707	1.17.1.4	NA
2	0.060	1o4zB	0.453	4.15	0.071	0.585	3.2.1.81	NA
3	0.060	2vy0A	0.462	4.17	0.058	0.603	3.2.1.39	NA
4	0.060	2ckjA	0.372	6.98	0.046	0.690	1.17.1.4 1.17.3.2	NA
5	0.060	3b9jJ	0.244	5.72	0.063	0.383	1.17.1.4 1.17.3.2	NA



(a) Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b) TM-score is a measure of global structural similarity between query and template protein.
(c) RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d) IDEN^a is the percentage sequence identity in the structurally aligned region.
(e) Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:


Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Predicted binding site residues
1	0.21	2iwg2	0.610	0.97	0.425	0.624	1.49	III	122,136,137,164,177,178,179,190,192,257,258,259,260
2	0.01	1bjqA	0.462	4.01	0.066	0.603	0.43	ADE	166,167,168,186,188
3	0.01	1lulA	0.447	3.90	0.049	0.578	0.45	CA	101,140,142



(a) Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b) BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c) TM-score is a measure of global structural similarity between query and template protein.
(d) RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e) IDEN^a is the percentage sequence identity in the structurally aligned region.
(f) Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

References:
1. Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.