D-I-TASSER A6NFC5

D-I-TASSER results for A6NFC5

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: A6NFC5
Protein Name: Transmembrane protein 235
Gene Name: TMEM235

Input Sequence in FASTA format

>A6NFC5 (223 residues)
MARLGALLLAAALGALLSFALLAAAVASDYWYILEVADAGNGSAWPGRAELLSSHSGLWR
ICEGQNGCIPLVDPFASESLDVSTSVQHLILLHRAVIVVLPLSLVLLVCGWICGLLSSLA
QSVSLLLFTGCYFLLGSVLTLAGVSIYISYSHLAFAETVQQYGPQHMQGVRVSFGWSMAL
AWGSCALEAFSGTLLLSAAWTLSLSPPICGHLSPQQVGGRGGD

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	A6NFC5-D1	1-223	223		MARLGALLLAAALGALLSFALLAAAVASDYWYILEVADAGNGSAWPGRAELLSSHSGLWRICEGQNGCIPLVDPFASESLDVSTSVQHLILLHRAVIVVLPLSLVLLVCGWICGLLSSLAQSVSLLLFTGCYFLLGSVLTLAGVSIYISYSHLAFAETVQQYGPQHMQGVRVSFGWSMALAWGSCALEAFSGTLLLSAAWTLSLSPPICGHLSPQQVGGRGGD

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.7942

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5vhxE	0.69	2.58	0.192	0.794	model1_5vhxE.pdb.gz
2	6ov2A	0.69	2.14	0.196	0.753	model1_6ov2A.pdb.gz
3	6akfA	0.69	1.97	0.195	0.758	model1_6akfA.pdb.gz
4	5b2gA	0.67	2.60	0.175	0.762	model1_5b2gA.pdb.gz
5	3x29A	0.67	1.98	0.202	0.731	model1_3x29A.pdb.gz
6	4p79A	0.66	2.08	0.167	0.727	model1_4p79A.pdb.gz
7	6qkcI	0.64	2.02	0.272	0.704	model1_6qkcI.pdb.gz
8	6wxrB	0.63	4.03	0.120	0.843	model1_6wxrB.pdb.gz
9	5vjsA	0.63	3.01	0.095	0.753	model1_5vjsA.pdb.gz
10	5kk2E	0.61	2.82	0.082	0.727	model1_5kk2E.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0004872	1.00	receptor activity
GO:0005245	0.90	voltage-gated calcium channel activity
GO:0016247	0.88	channel regulator activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0016032	1.00	viral process
GO:0044699	0.96	single-organism process
GO:0065007	0.95	biological regulation
GO:0032501	0.92	multicellular organismal process
GO:0044707	0.90	single-multicellular organism process
GO:0006810	0.90	transport
GO:0003008	0.90	system process
GO:0050789	0.89	regulation of biological process
GO:0035637	0.89	multicellular organismal signaling
GO:0019226	0.88	transmission of nerve impulse
GO:0050794	0.87	regulation of cellular process
GO:0032879	0.85	regulation of localization
GO:0065009	0.84	regulation of molecular function
GO:0043269	0.83	regulation of ion transport
GO:0009966	0.83	regulation of signal transduction
GO:2000311	0.82	regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
GO:0006816	0.77	calcium ion transport
GO:0070588	0.76	calcium ion transmembrane transport

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0031466	1.00	Cul5-RING ubiquitin ligase complex
GO:0044425	0.93	membrane part
GO:0044459	0.91	plasma membrane part
GO:0032281	0.82	AMPA glutamate receptor complex

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1qd1B	0.248	6.37	0.039	0.462	2.1.2.5 4.3.1.4	NA
2	0.060	2epoB	0.446	4.86	0.039	0.637	3.2.1.52	NA
3	0.060	2epoA	0.473	4.65	0.049	0.677	3.2.1.52	NA
4	0.060	1jswB	0.493	4.95	0.038	0.727	4.3.1.1	NA
5	0.060	1j3uA	0.497	4.64	0.066	0.709	4.3.1.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	1e3dB	0.486	4.51	0.048	0.700	0.87	H2S	complex1.pdb.gz	111,129,132,133
2	0.01	2jbsC	0.488	4.39	0.065	0.677	0.62	FMN	complex2.pdb.gz	129,137,193,194
3	0.01	1yqwQ	0.504	4.17	0.046	0.686	0.51	UUU	complex3.pdb.gz	12,16,110,111,131,132,133,188,191
4	0.01	1fupB	0.491	4.63	0.071	0.718	0.51	PMA	complex4.pdb.gz	137,184,186
5	0.01	1h2rL	0.501	4.21	0.061	0.700	0.54	NFE	complex5.pdb.gz	17,107,135,136,137,185,188
6	0.01	1fupA	0.490	4.74	0.070	0.722	0.51	PMA	complex6.pdb.gz	137,141,182,188

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.