D-I-TASSER results for A0M8Q6

Summary of the Protein

Uniprot ID: A0M8Q6


Protein Name: Immunoglobulin lambda constant 7


Gene Name: IGLC7

Input Sequence in FASTA format

>A0M8Q6 (106 residues)
GQPKAAPSVTLFPPSSEELQANKATLVCLVSDFNPGAVTVAWKADGSPVKVGVETTKPSK
QSNNKYAASSYLSLTPEQWKSHRSYSCRVTHEGSTVEKTVAPAECS

Predicted Contact and Distance Map Used in D-I-TASSER simulation

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Sequence
1	A0M8Q6-D1	1-106	106	GQPKAAPSVTLFPPSSEELQANKATLVCLVSDFNPGAVTVAWKADGSPVKVGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCRVTHEGSTVEKTVAPAECS

Top 1 final models from D-I-TASSER

Rank^a	Estimated TM-score^b
1	0.8776


(a) D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.
(b) The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)


Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.
1	2rcjT	0.96	0.97	0.925	0.991
2	6qn7L	0.96	0.82	0.752	0.991
3	7jkbA2	0.95	0.63	0.922	0.972
4	7cr5L	0.95	0.53	0.941	0.962
5	1nc4C	0.95	1.12	0.695	0.991
6	6mtsL	0.95	0.67	0.922	0.972
7	6ce0E	0.94	0.80	0.913	0.981
8	1a8jL	0.94	1.31	0.934	1.000
9	5ocyL	0.94	0.93	0.702	0.981
10	6oz2L	0.94	0.76	0.932	0.972



(a) Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b) Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c) RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d) IDEN^a is the percentage sequence identity in the structurally aligned region.
(e) Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)


GO term	Cscore^GO	Name
GO:0003823	0.87	antigen binding
GO:0005102	0.70	receptor binding
GO:0034987	0.65	immunoglobulin receptor binding






(a) Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

Biological Process (BP)


GO term	Cscore^GO	Name
GO:0002376	0.97	immune system process
GO:0002682	0.86	regulation of immune system process
GO:0048518	0.84	positive regulation of biological process
GO:0050896	0.83	response to stimulus
GO:0050794	0.83	regulation of cellular process
GO:0002694	0.82	regulation of leukocyte activation
GO:0002684	0.82	positive regulation of immune system process
GO:0048522	0.81	positive regulation of cellular process
GO:0006955	0.79	immune response
GO:0002696	0.79	positive regulation of leukocyte activation
GO:0048583	0.73	regulation of response to stimulus
GO:0051249	0.72	regulation of lymphocyte activation
GO:0048584	0.72	positive regulation of response to stimulus
GO:0050778	0.70	positive regulation of immune response
GO:0051251	0.68	positive regulation of lymphocyte activation
GO:0006959	0.66	humoral immune response
GO:0002253	0.66	activation of immune response
GO:0002252	0.66	immune effector process
GO:0050851	0.65	antigen receptor-mediated signaling pathway
GO:0043207	0.65	response to external biotic stimulus
GO:0009987	0.65	cellular process
GO:0051707	0.64	response to other organism
GO:0006952	0.64	defense response
GO:0016043	0.63	cellular component organization
GO:0009617	0.63	response to bacterium
GO:0044699	0.62	single-organism process
GO:0044763	0.61	single-organism cellular process
GO:0042742	0.61	defense response to bacterium
GO:0050871	0.60	positive regulation of B cell activation
GO:0006958	0.60	complement activation, classical pathway
GO:0006911	0.60	phagocytosis, engulfment
GO:0006910	0.60	phagocytosis, recognition
GO:0050853	0.59	B cell receptor signaling pathway
GO:0045087	0.59	innate immune response






(a) Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

Cellular Component (CC)


GO term	Cscore^GO	Name
GO:0044464	0.86	cell part
GO:0044425	0.81	membrane part
GO:0044459	0.77	plasma membrane part
GO:0044421	0.76	extracellular region part
GO:0043234	0.72	protein complex
GO:0005576	0.70	extracellular region
GO:0009897	0.68	external side of plasma membrane
GO:0042571	0.60	immunoglobulin complex, circulating
GO:0072562	0.59	blood microparticle
GO:0016020	0.53	membrane






(a) Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.
(b) The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB


Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.161	1tnnA	0.570	3.30	0.186	0.792	2.7.11.1	39,97
2	0.091	2bk8A	0.649	2.83	0.102	0.830	2.7.11.1	NA
3	0.086	2wwmD	0.651	2.75	0.169	0.840	2.7.11.1	NA
4	0.067	2gg1A	0.553	2.75	0.098	0.745	2.7.7.48 3.4.21.91	NA
5	0.067	2rq8A	0.582	2.65	0.037	0.774	2.7.11.1	NA



(a) Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b) TM-score is a measure of global structural similarity between query and template protein.
(c) RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d) IDEN^a is the percentage sequence identity in the structurally aligned region.
(e) Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:


Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Predicted binding site residues
1	0.43	2h3n0	0.930	0.72	0.824	0.962	1.61	III	10,12,13,23,25,27,29,54,56,57,59,69,71
2	0.26	1pg74	0.927	0.69	0.693	0.953	0.84	III	12,13,14,15,17,18,21,25,27,29,31,32,54,56,57,61,68,69,71,99
3	0.13	1mco1	0.821	2.28	0.854	0.962	0.70	III	8,14,15,17,27,28,29,30,31,56,57,58,59,60,61,62,67,68,69,70,71



(a) Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b) BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c) TM-score is a measure of global structural similarity between query and template protein.
(d) RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e) IDEN^a is the percentage sequence identity in the structurally aligned region.
(f) Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

References:
1. Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.