D-I-TASSER A0A0J9YXV3

D-I-TASSER results for A0A0J9YXV3

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: A0A0J9YXV3
Protein Name: Long intergenic non-protein coding RNA 514
Gene Name: LINC00514

Input Sequence in FASTA format

>A0A0J9YXV3 (536 residues)
MGAWAFPAALFLLCLTSESLQGGLPLLPPGLGKVYGPHSGLGAGYDGGVKPQKPGFVVRH
GLGTQPDTEGGMKPQNLGFRTFAGAAAQPGYGNGLGAAAFPVAGAQSGPAAQNGFGPGFG
GGGKPQKPGPTTQNGYRPGYVGAVKPQKPGFQYRIGLGAQPGFRGDMKAQEPGLGNGNGL
SAQPVLTAQNRFGFGAGLGGNVKPLKPGYGKRLRAGAFPGAGTQPEYGHGNGPGVQPGLG
AGMKPQMPGLGAPNGYGPGRGRAGVPGGPERRPWVPHLLPFSSPGYLGVMKAQKPGPLAQ
NGYRAGAGEGMKPQKPGLRGTLKPQKSGHGHENGPWPGPCNARVAPMLLPRLPTPGVPSD
KEGGWGLKSQPPSAVQNGKLPAPMPAIQWGLKPQKAGHQPPNGYGPGAEPGFNGGLEPQK
IGLGYGNGVLGARVFPEAHPQPGFHGANGFRNRDGVEALVYPKAAALAPEGNGQAGVLWN
SRWPTLQAWGAGLKPGYQAGDEYAEARSQPGGPDVKRGSNGQLGNGYGGRCPLGKC

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	A0A0J9YXV3-D1	1-149	149		MGAWAFPAALFLLCLTSESLQGGLPLLPPGLGKVYGPHSGLGAGYDGGVKPQKPGFVVRHGLGTQPDTEGGMKPQNLGFRTFAGAAAQPGYGNGLGAAAFPVAGAQSGPAAQNGFGPGFGGGGKPQKPGPTTQNGYRPGYVGAVKPQKP
2	A0A0J9YXV3-D2	150-419	270		GFQYRIGLGAQPGFRGDMKAQEPGLGNGNGLSAQPVLTAQNRFGFGAGLGGNVKPLKPGYGKRLRAGAFPGAGTQPEYGHGNGPGVQPGLGAGMKPQMPGLGAPNGYGPGRGRAGVPGGPERRPWVPHLLPFSSPGYLGVMKAQKPGPLAQNGYRAGAGEGMKPQKPGLRGTLKPQKSGHGHENGPWPGPCNARVAPMLLPRLPTPGVPSDKEGGWGLKSQPPSAVQNGKLPAPMPAIQWGLKPQKAGHQPPNGYGPGAEPGFNGGLEPQ
3	A0A0J9YXV3-D3	420-536	117		KIGLGYGNGVLGARVFPEAHPQPGFHGANGFRNRDGVEALVYPKAAALAPEGNGQAGVLWNSRWPTLQAWGAGLKPGYQAGDEYAEARSQPGGPDVKRGSNGQLGNGYGGRCPLGKC

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.35

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5wbiA	0.35	7.50	0.056	0.563	model1_5wbiA.pdb.gz
2	6ulwA	0.35	7.75	0.035	0.577	model1_6ulwA.pdb.gz
3	6bcuW	0.35	7.60	0.045	0.569	model1_6bcuW.pdb.gz
4	3ahcA	0.35	7.59	0.050	0.571	model1_3ahcA.pdb.gz
5	5m59A	0.35	8.31	0.033	0.612	model1_5m59A.pdb.gz
6	6mu1A	0.35	7.80	0.039	0.582	model1_6mu1A.pdb.gz
7	6lxvA	0.35	7.74	0.048	0.584	model1_6lxvA.pdb.gz
8	5kf6A	0.35	8.13	0.054	0.595	model1_5kf6A.pdb.gz
9	7bdiB	0.35	7.99	0.051	0.586	model1_7bdiB.pdb.gz
10	6uebA	0.35	7.91	0.042	0.588	model1_6uebA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0043169	0.40	cation binding
GO:0005198	0.36	structural molecule activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0044699	0.82	single-organism process
GO:0009987	0.74	cellular process
GO:0032502	0.61	developmental process
GO:0044763	0.59	single-organism cellular process
GO:0044767	0.56	single-organism developmental process
GO:0048856	0.55	anatomical structure development

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0044421	0.69	extracellular region part
GO:0044444	0.68	cytoplasmic part
GO:0031012	0.48	extracellular matrix
GO:0005829	0.48	cytosol
GO:0043226	0.46	organelle
GO:0043227	0.45	membrane-bounded organelle
GO:0032991	0.44	macromolecular complex
GO:0043234	0.43	protein complex
GO:0044446	0.41	intracellular organelle part
GO:0043229	0.41	intracellular organelle
GO:0043231	0.40	intracellular membrane-bounded organelle
GO:0016020	0.40	membrane
GO:0005737	0.40	cytoplasm
GO:0005581	0.33	collagen trimer
GO:0031982	0.32	vesicle
GO:0031988	0.31	membrane-bounded vesicle
GO:0070062	0.30	extracellular exosome
GO:0005578	0.30	proteinaceous extracellular matrix
GO:0005576	0.30	extracellular region

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1gaxA	0.316	7.60	0.049	0.520	6.1.1.9	177
2	0.060	1h0hA	0.306	7.25	0.044	0.485	1.2.1.2	NA
3	0.060	1kc7A	0.337	8.05	0.051	0.580	2.7.9.1	179
4	0.060	2hivA	0.326	7.54	0.042	0.530	6.5.1.1	NA
5	0.060	1wz2A	0.307	8.03	0.046	0.528	6.1.1.4	78

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	2g25A	0.359	7.49	0.045	0.582	0.25	TDK	complex1.pdb.gz	40,151,158,159,160,183
2	0.01	3cmvD	0.326	7.80	0.030	0.548	0.26	ANP	complex2.pdb.gz	39,40,41,42,43,44,45
3	0.01	3ai7E	0.346	7.76	0.044	0.580	0.23	TPP	complex3.pdb.gz	39,157,159,211,213,216
4	0.01	3lq4B	0.348	7.64	0.037	0.573	0.22	TDP	complex4.pdb.gz	12,17,39,40,41,42,61
5	0.01	3cmvF	0.302	8.13	0.047	0.528	0.29	ANP	complex5.pdb.gz	78,79,80,99,120
6	0.01	2qtcB	0.348	7.61	0.037	0.573	0.23	TDK	complex6.pdb.gz	31,158,159,160,183
7	0.01	3cmvB	0.324	7.81	0.032	0.545	0.32	ANP	complex7.pdb.gz	39,40,61,105
8	0.01	3cmvG	0.317	8.09	0.045	0.550	0.22	ANP	complex8.pdb.gz	43,44,45,102
9	0.01	3cmvA	0.294	7.94	0.040	0.504	0.27	ANP	complex9.pdb.gz	76,77,78,79,109
10	0.01	2g28A	0.345	7.76	0.034	0.577	0.27	TDK	complex10.pdb.gz	17,104,105,301,303,304

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.