6nfn A 2.63 BS01 BCG B 1 Y34 G51 A52 W93 Y34 G51 A52 W93 31702583 301 QAVVIQESALTTSPGGTVILTCRSSTGTITTSNYANWVQKKPNHVFTGLIGATSIRAPGVPVRFSGFLIGGKAALTITGAQTEDDAMYFCALWYNTHYVFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTE 6nfn B 2.63 BS01 BCG B 1 W33 E99 L100 W33 E99 L100 31702583 301 EVQLVESGGGLVQPGGSLRLSCAASGFIFSSDWMNWVRQAPGKGLEWVANINQDGSEKYYVDSVKGRFTISRDNAQNSLYLQMNSLRAEDTAVYYCAKELGPWGQGTLVTVSSASTKGPSVFPLAPSAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK 6nfn C 2.63 BS01 BCG C 1 Y34 A52 W93 Y98 Y33 A51 W92 Y97 31702583 301 AVVIQESALTTSPGGTVILTCRSSTGTITTSNYANWVQKKPNHVFTGLIGATSIRAPGVPVRFSGFLIGGKAALTITGAQTEDDAMYFCALWYNTHYVFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTE 6nfn D 2.63 BS01 BCG C 1 W33 E99 L100 W33 E99 L100 31702583 301 EVQLVESGGGLVQPGGSLRLSCAASGFIFSSDWMNWVRQAPGKGLEWVANINQDGSEKYYVDSVKGRFTISRDNAQNSLYLQMNSLRAEDTAVYYCAKELGPWGQGTLVTVSSASTKGPSVFPLAPSSGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK 6nfn E 2.63 BS01 BCG E 1 Y34 A52 W93 Y98 Y33 A51 W92 Y97 31702583 301 AVVIQESALTTSPGGTVILTCRSSTGTITTSNYANWVQKKPNHVFTGLIGATSIRAPGVPVRFSGFLIGGKAALTITGAQTEDDAMYFCALWYNTHYVFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEC 6nfn F 2.63 BS01 BCG E 1 W33 E99 L100 W33 E99 L100 31702583 301 EVQLVESGGGLVQPGGSLRLSCAASGFIFSSDWMNWVRQAPGKGLEWVANINQDGSEKYYVDSVKGRFTISRDNAQNSLYLQMNSLRAEDTAVYYCAKELGPWGQGTLVTVSSASTKGPSVFPLAPSSKGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC 6nfn H 2.63 BS01 BCG L 1 W33 E99 L100 W33 E99 L100 -logKd/Ki=7.70, Kd=20nM 31702583 301 EVQLVESGGGLVQPGGSLRLSCAASGFIFSSDWMNWVRQAPGKGLEWVANINQDGSEKYYVDSVKGRFTISRDNAQNSLYLQMNSLRAEDTAVYYCAKELGPWGQGTLVTVSSASTKGPSVFPLAPSALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP 6nfn I 2.63 BS01 BCG J 1 Y34 W93 Y98 Y33 W92 Y97 31702583 301 AVVIQESALTTSPGGTVILTCRSSTGTITTSNYANWVQKKPNHVFTGLIGATSIRAPGVPVRFSGFLIGGKAALTITGAQTEDDAMYFCALWYNTHYVFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEC 6nfn J 2.63 BS01 BCG J 1 W33 E99 L100 W33 E99 L100 31702583 301 EVQLVESGGGLVQPGGSLRLSCAASGFIFSSDWMNWVRQAPGKGLEWVANINQDGSEKYYVDSVKGRFTISRDNAQNSLYLQMNSLRAEDTAVYYCAKELGPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHH 6nfn L 2.63 BS01 BCG L 1 Y34 A52 W93 Y98 Y32 A50 W91 Y96 -logKd/Ki=7.70, Kd=20nM 31702583 301 VVIQESALTTSPGGTVILTCRSSTGTITTSNYANWVQKKPNHVFTGLIGATSIRAPGVPVRFSGFLIGGKAALTITGAQTEDDAMYFCALWYNTHYVFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTE 6nfn M 2.63 BS01 BCG M 1 Y34 W93 Y32 W91 31702583 301 VVIQESALTTSPGGTVILTCRSSTGTITTSNYANWVQKKPNHVFTGLIGATSIRAPGVPVRFSGFLIGGKAALTITGAQTEDDAMYFCALWYNTHYVFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS 6nfn N 2.63 BS01 BCG M 1 W33 E99 L100 W33 E99 L100 31702583 301 EVQLVESGGGLVQPGGSLRLSCAASGFIFSSDWMNWVRQAPGKGLEWVANINQDGSEKYYVDSVKGRFTISRDNAQNSLYLQMNSLRAEDTAVYYCAKELGPWGQGTLVTVSSASTKGPSVFPLAPTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH 6nfn O 2.63 BS01 BCG O 1 Y34 A52 W93 Y98 Y34 A52 W93 Y98 31702583 301 QAVVIQESALTTSPGGTVILTCRSSTGTITTSNYANWVQKKPNHVFTGLIGATSIRAPGVPVRFSGFLIGGKAALTITGAQTEDDAMYFCALWYNTHYVFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT 6nfn P 2.63 BS01 BCG O 1 W33 E99 L100 W33 E99 L100 31702583 301 EVQLVESGGGLVQPGGSLRLSCAASGFIFSSDWMNWVRQAPGKGLEWVANINQDGSEKYYVDSVKGRFTISRDNAQNSLYLQMNSLRAEDTAVYYCAKELGPWGQGTLVTVSSASTKGPSVFPLAPSSKTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK 6nfn Q 2.63 BS01 BCG Q 1 Y34 A52 W93 Y98 Y33 A51 W92 Y97 31702583 301 AVVIQESALTTSPGGTVILTCRSSTGTITTSNYANWVQKKPNHVFTGLIGATSIRAPGVPVRFSGFLIGGKAALTITGAQTEDDAMYFCALWYNTHYVFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAP 6nfn R 2.63 BS01 BCG Q 1 W33 E99 L100 W33 E99 L100 31702583 301 EVQLVESGGGLVQPGGSLRLSCAASGFIFSSDWMNWVRQAPGKGLEWVANINQDGSEKYYVDSVKGRFTISRDNAQNSLYLQMNSLRAEDTAVYYCAKELGPWGQGTLVTVSSASTKGPSVFPLAPAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKK