6n96 A 1.7 BS01 LCV A 1 K60 A64 H66 D67 I68 H69 W108 G109 G110 T132 P133 L136 V138 Y140 F250 K253 K59 A63 H65 D66 I67 H68 W107 G108 G109 T131 P132 L135 V137 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 A 1.7 BS02 SO5 A 1 K60 A64 H66 D67 I68 H69 W108 G109 G110 T132 P133 L136 V138 Y140 F250 K253 K59 A63 H65 D66 I67 H68 W107 G108 G109 T131 P132 L135 V137 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 302 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 B 1.7 BS01 LCV B 1 A64 H66 D67 I68 G109 G110 T132 P133 L136 V138 Y140 F250 K253 A63 H65 D66 I67 G108 G109 T131 P132 L135 V137 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 B 1.7 BS02 SO5 B 1 A64 H66 D67 I68 W108 G109 G110 T132 P133 V138 Y140 F250 K253 A63 H65 D66 I67 W107 G108 G109 T131 P132 V137 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 302 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 C 1.7 BS01 LCV C 1 L25 A64 H66 D67 I68 W108 G109 G110 T132 P133 L136 Y140 L24 A63 H65 D66 I67 W107 G108 G109 T131 P132 L135 Y139 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM -logKd/Ki=5.42, Ki=3.8uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 C 1.7 BS02 SO5 C 1 L25 A64 H66 D67 I68 W108 G109 G110 T132 P133 L136 Y140 L24 A63 H65 D66 I67 W107 G108 G109 T131 P132 L135 Y139 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 302 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 C 1.7 BS03 LCV C 2 W108 T128 F129 S130 S165 P166 W107 T127 F128 S129 S164 P165 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM -logKd/Ki=5.42, Ki=3.8uM P52045 30869886 303 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 D 1.7 BS01 LCV D 1 K60 A64 H66 D67 I68 G109 G110 T132 P133 L136 V138 Y140 F250 K253 K59 A63 H65 D66 I67 G108 G109 T131 P132 L135 V137 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 D 1.7 BS02 SO5 D 1 K60 A64 H66 D67 I68 G109 G110 T132 P133 L136 V138 Y140 F250 K253 K59 A63 H65 D66 I67 G108 G109 T131 P132 L135 V137 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 302 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 E 1.7 BS01 LCV E 1 A64 H66 D67 I68 W108 G109 G110 T132 P133 L136 V138 Y140 F250 K253 A63 H65 D66 I67 W107 G108 G109 T131 P132 L135 V137 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 E 1.7 BS02 SO5 E 1 A64 H66 D67 I68 W108 G109 G110 T132 P133 L136 V138 Y140 F250 K253 A63 H65 D66 I67 W107 G108 G109 T131 P132 L135 V137 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 302 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 F 1.7 BS01 LCV F 1 A64 G65 H66 D67 I68 W108 G109 G110 T132 P133 L136 V138 Y140 F250 K253 A63 G64 H65 D66 I67 W107 G108 G109 T131 P132 L135 V137 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 F 1.7 BS02 SO5 F 1 A64 H66 D67 I68 W108 G109 G110 T132 P133 L136 Y140 F250 K253 A63 H65 D66 I67 W107 G108 G109 T131 P132 L135 Y139 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 302 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n96 F 1.7 BS03 LCV F 2 W108 T128 F129 S130 S165 P166 W107 T127 F128 S129 S164 P165 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=3.8uM P52045 30869886 303 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH