6n93 A 1.7 BS01 KGA A 1 L25 A64 H66 D67 I68 H69 W108 T132 L136 F250 K253 L24 A63 H65 D66 I67 H68 W107 T131 L135 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=20.6uM -logKd/Ki=4.69, Kd=20.6uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n93 B 1.7 BS01 KGA B 1 L25 A64 H66 D67 I68 W108 F250 K253 L24 A63 H65 D66 I67 W107 F249 K252 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=20.6uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n93 C 1.7 BS01 KGA C 1 K24 L25 A64 H66 D67 I68 W108 T132 L136 F250 K23 L24 A63 H65 D66 I67 W107 T131 L135 F249 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=20.6uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n93 C 1.7 BS02 KGA C 2 W108 T128 F129 S130 S165 P166 W107 T127 F128 S129 S164 P165 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=20.6uM P52045 30869886 303 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n93 D 1.7 BS01 KGA D 1 K60 A64 H66 D67 I68 W108 L136 K59 A63 H65 D66 I67 W107 L135 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=20.6uM P52045 30869886 302 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n93 E 1.7 BS01 KGA E 1 K24 L25 A64 G65 H66 D67 I68 W108 G109 G110 T132 P133 F250 K23 L24 A63 G64 H65 D66 I67 W107 G108 G109 T131 P132 F249 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=20.6uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n93 E 1.7 BS02 KGA E 2 V107 W108 T128 S130 S165 P166 V106 W107 T127 S129 S164 P165 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=20.6uM P52045 30869886 302 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n93 F 1.7 BS01 KGA F 1 L25 A64 H66 D67 I68 W108 G109 G110 T132 F250 L24 A63 H65 D66 I67 W107 G108 G109 T131 F249 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=20.6uM P52045 30869886 301 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n93 F 1.7 BS02 KGA F 2 S106 V107 W108 T128 F129 S130 S165 P166 S105 V106 W107 T127 F128 S129 S164 P165 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 Ki=20.6uM P52045 30869886 303 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH 6n93 F 1.7 BS03 KG7 F 1 E241 H261 E240 H260 H66 L71 G110 E113 P133 V138 Y140 E228 Y238 H65 L70 G109 E112 P132 V137 Y139 E227 Y237 4.1.1.- 0003824,0004492,0005829,0006635,0016829,0016831 P52045 30869886 304 AYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLNRPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH