4elf A 2.3 BS01 CA A 1 Y108 D110 Y108 D110 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Q81R22 22999981 201 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf A 2.3 BS02 35I A 1 M6 V7 A8 L21 E28 L29 V32 K33 I51 P56 R58 F96 M6 V7 A8 L21 E28 L29 V32 K33 I51 P56 R58 F96 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Ki=9.4nM -logKd/Ki=8.03, Ki=9.4nM Q81R22 22999981 203 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf B 2.3 BS01 CA B 1 Y108 D110 Y108 D110 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Q81R22 22999981 201 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf B 2.3 BS02 35I B 1 M6 V7 A8 L21 E28 L29 Q30 V32 K33 I51 L55 P56 R58 F96 M6 V7 A8 L21 E28 L29 Q30 V32 K33 I51 L55 P56 R58 F96 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Ki=9.4nM Q81R22 22999981 203 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf C 2.3 BS01 CA C 1 Y108 D110 Y108 D110 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Q81R22 22999981 201 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf C 2.3 BS02 35I C 1 M6 V7 A8 L21 E28 L29 Q30 V32 K33 I51 L55 P56 R58 F96 M6 V7 A8 L21 E28 L29 Q30 V32 K33 I51 L55 P56 R58 F96 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Ki=9.4nM Q81R22 22999981 203 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf D 2.3 BS01 CA D 1 Y108 D110 Y108 D110 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Q81R22 22999981 201 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf D 2.3 BS02 35I D 1 M6 V7 A8 L21 E28 L29 V32 K33 I51 R53 L55 P56 R58 F96 M6 V7 A8 L21 E28 L29 V32 K33 I51 R53 L55 P56 R58 F96 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Ki=9.4nM Q81R22 22999981 203 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf E 2.3 BS01 CA E 1 Y108 D110 Y108 D110 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Q81R22 22999981 201 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf E 2.3 BS02 35I E 1 M6 V7 A8 L21 E28 L29 V32 K33 A50 I51 L55 R58 F96 M6 V7 A8 L21 E28 L29 V32 K33 A50 I51 L55 R58 F96 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Ki=9.4nM Q81R22 22999981 203 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf F 2.3 BS01 CA F 1 Y108 D110 Y108 D110 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Q81R22 22999981 201 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf F 2.3 BS02 35I F 1 M6 V7 A8 L21 E28 L29 Q30 V32 K33 I51 R53 L55 R58 F96 M6 V7 A8 L21 E28 L29 Q30 V32 K33 I51 R53 L55 R58 F96 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Ki=9.4nM Q81R22 22999981 203 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf G 2.3 BS01 CA G 1 Y108 D110 Y108 D110 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Q81R22 22999981 201 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf G 2.3 BS02 35I G 1 M6 V7 A8 N20 L21 E28 L29 Q30 V32 K33 R53 L55 P56 R58 F96 M6 V7 A8 N20 L21 E28 L29 Q30 V32 K33 R53 L55 P56 R58 F96 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Ki=9.4nM Q81R22 22999981 203 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf H 2.3 BS01 35I H 1 M6 V7 A8 L21 E28 L29 Q30 V32 K33 I51 L55 P56 R58 F96 M6 V7 A8 L21 E28 L29 Q30 V32 K33 I51 L55 P56 R58 F96 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Ki=9.4nM Q81R22 22999981 202 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR 4elf H 2.3 BS02 CA H 1 Y108 D110 Y108 D110 M6 L21 W23 E28 L29 V32 L55 I93 T115 M6 L21 W23 E28 L29 V32 L55 I93 T115 1.5.1.3 0000166,0004146,0005829,0006730,0016491,0046452,0046654,0046655,0046872,0050661 Q81R22 22999981 203 MIVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRPLPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEEIFIFGGAQIYDLFLPYVDKLYITKIHHAFEGDTFFPEMDMTNWKEVFVEKGLTDEKNPYTYYYHVYEKQQLVPR