4ear A 1.7 BS01 IM5 A 1 Y88 A116 A117 G118 F200 E201 V217 M219 N243 X257 Y85 A113 A114 G115 F197 E198 V214 M216 N240 X254 S33 H64 H86 Y88 E89 A116 M219 S220 N243 V245 W257 S30 H61 H83 Y85 E86 A113 M216 S217 N240 V242 W254 2.4.2.1 0000255,0001882,0002060,0003824,0004731,0005515,0005576,0005737,0005829,0006139,0006148,0006149,0006157,0006166,0006204,0006738,0006955,0009116,0009165,0009410,0016757,0016763,0032743,0034418,0034774,0042102,0042301,0042802,0043101,0046059,0046638,0047975,0070062,1904813 -logKd/Ki=9.82, Kd=151pM Ki=7nM P00491 23438750 301 GYTYEDYKNTAEYLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLYKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTYKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANWEEVLAAGKQAAQKLEQFVSILMASIPLPD 4ear A 1.7 BS02 PO4 A 1 G32 S33 R84 H86 N115 S220 G29 S30 R81 H83 N112 S217 S33 H64 H86 Y88 E89 A116 M219 S220 N243 V245 W257 S30 H61 H83 Y85 E86 A113 M216 S217 N240 V242 W254 2.4.2.1 0000255,0001882,0002060,0003824,0004731,0005515,0005576,0005737,0005829,0006139,0006148,0006149,0006157,0006166,0006204,0006738,0006955,0009116,0009165,0009410,0016757,0016763,0032743,0034418,0034774,0042102,0042301,0042802,0043101,0046059,0046638,0047975,0070062,1904813 P00491 23438750 302 GYTYEDYKNTAEYLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLYKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTYKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANWEEVLAAGKQAAQKLEQFVSILMASIPLPD 4ear B 1.7 BS01 IM5 B 1 Y88 A116 A117 G118 F200 E201 V217 M219 N243 X257 V260 Y87 A115 A116 G117 F199 E200 V216 M218 N242 X256 V259 S33 H64 H86 Y88 E89 A116 M219 S220 N243 V245 W257 S32 H63 H85 Y87 E88 A115 M218 S219 N242 V244 W256 2.4.2.1 0000255,0001882,0002060,0003824,0004731,0005515,0005576,0005737,0005829,0006139,0006148,0006149,0006157,0006166,0006204,0006738,0006955,0009116,0009165,0009410,0016757,0016763,0032743,0034418,0034774,0042102,0042301,0042802,0043101,0046059,0046638,0047975,0070062,1904813 Ki=7nM P00491 23438750 301 ENGYTYEDYKNTAEYLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLYKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTYKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANWEEVLAAGKQAAQKLEQFVSILMASIPLPDKA 4ear B 1.7 BS02 PO4 B 1 G32 S33 R84 H86 N115 S220 G31 S32 R83 H85 N114 S219 S33 H64 H86 Y88 E89 A116 M219 S220 N243 V245 W257 S32 H63 H85 Y87 E88 A115 M218 S219 N242 V244 W256 2.4.2.1 0000255,0001882,0002060,0003824,0004731,0005515,0005576,0005737,0005829,0006139,0006148,0006149,0006157,0006166,0006204,0006738,0006955,0009116,0009165,0009410,0016757,0016763,0032743,0034418,0034774,0042102,0042301,0042802,0043101,0046059,0046638,0047975,0070062,1904813 P00491 23438750 302 ENGYTYEDYKNTAEYLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLYKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTYKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANWEEVLAAGKQAAQKLEQFVSILMASIPLPDKA 4ear C 1.7 BS01 IM5 C 1 S33 Y88 A116 A117 G118 F200 E201 M219 N243 X257 S30 Y85 A113 A114 G115 F197 E198 M216 N240 X254 S33 H64 H86 Y88 E89 A116 M219 S220 N243 V245 W257 S30 H61 H83 Y85 E86 A113 M216 S217 N240 V242 W254 2.4.2.1 0000255,0001882,0002060,0003824,0004731,0005515,0005576,0005737,0005829,0006139,0006148,0006149,0006157,0006166,0006204,0006738,0006955,0009116,0009165,0009410,0016757,0016763,0032743,0034418,0034774,0042102,0042301,0042802,0043101,0046059,0046638,0047975,0070062,1904813 -logKd/Ki=9.82, Kd=151pM Ki=7nM P00491 23438750 301 GYTYEDYKNTAEYLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLYKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTYKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANWEEVLAAGKQAAQKLEQFVSILMASIPLPDKA 4ear C 1.7 BS02 PO4 C 1 G32 S33 R84 H86 N115 S220 G29 S30 R81 H83 N112 S217 S33 H64 H86 Y88 E89 A116 M219 S220 N243 V245 W257 S30 H61 H83 Y85 E86 A113 M216 S217 N240 V242 W254 2.4.2.1 0000255,0001882,0002060,0003824,0004731,0005515,0005576,0005737,0005829,0006139,0006148,0006149,0006157,0006166,0006204,0006738,0006955,0009116,0009165,0009410,0016757,0016763,0032743,0034418,0034774,0042102,0042301,0042802,0043101,0046059,0046638,0047975,0070062,1904813 P00491 23438750 302 GYTYEDYKNTAEYLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLYKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTYKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANWEEVLAAGKQAAQKLEQFVSILMASIPLPDKA