3rv8 A 2.29 BS01 RVC A 1 L268 T361 Y385 L404 R405 G419 K438 L254 T329 Y353 L372 R373 G387 K406 K205 E252 A269 E297 H334 T361 Y385 R405 G421 E434 K438 K191 E238 A255 E269 H302 T329 Y353 R373 G389 E402 K406 4.2.99.21,5.4.4.2,5.4.99.5 0000162,0000287,0004106,0005886,0008909,0009058,0009697,0010106,0016829,0016833,0016853,0019540,0043904,0046872,0052572 Ki=12uM -logKd/Ki=4.92, Ki=12uM P9WFX1 22607697 451 SSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTRRQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAIDRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQLGGIRALGYSPELVTAVRADGVVITEPLAGTDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAVIDFMTVRVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVARQ 3rv8 B 2.29 BS01 RVC B 1 L404 R405 A418 K438 L386 R387 A400 K420 K205 E252 A269 E297 H334 T361 Y385 R405 G421 E434 K438 K191 E238 A255 E279 H316 T343 Y367 R387 G403 E416 K420 4.2.99.21,5.4.4.2,5.4.99.5 0000162,0000287,0004106,0005886,0008909,0009058,0009697,0010106,0016829,0016833,0016853,0019540,0043904,0046872,0052572 Ki=12uM P9WFX1 22607697 451 SSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTRRQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAIDRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQLGGIRALGYSPELVTAVRADGVVITEPLAGTRALGRGRLARDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAVIDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVARQ 3rv8 C 2.29 BS01 RVC C 1 L268 H334 T361 L404 R405 K438 L254 H315 T342 L385 R386 K419 K205 E252 A269 E297 H334 T361 Y385 R405 G421 E434 K438 K191 E238 A255 E278 H315 T342 Y366 R386 G402 E415 K419 4.2.99.21,5.4.4.2,5.4.99.5 0000162,0000287,0004106,0005886,0008909,0009058,0009697,0010106,0016829,0016833,0016853,0019540,0043904,0046872,0052572 Ki=12uM P9WFX1 22607697 451 SSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTRRQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAIDRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQLGGIRALGYSPELVTAVRADGVVITEPLAGTGPAIDRLARDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAVIDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVAR 3rv8 C 2.29 BS02 VCE C 1 H334 T361 Y385 L404 R405 A418 K438 H315 T342 Y366 L385 R386 A399 K419 K205 E252 A269 E297 H334 T361 Y385 R405 G421 E434 K438 K191 E238 A255 E278 H315 T342 Y366 R386 G402 E415 K419 4.2.99.21,5.4.4.2,5.4.99.5 0000162,0000287,0004106,0005886,0008909,0009058,0009697,0010106,0016829,0016833,0016853,0019540,0043904,0046872,0052572 Ki=12uM P9WFX1 22607697 1451 SSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTRRQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAIDRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQLGGIRALGYSPELVTAVRADGVVITEPLAGTGPAIDRLARDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAVIDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVAR 3rv8 D 2.29 BS01 RVC D 1 L404 R405 G419 K438 L375 R376 G390 K409 K205 E252 A269 E297 H334 T361 Y385 R405 G421 E434 K438 K192 E239 A256 E272 H305 T332 Y356 R376 G392 E405 K409 4.2.99.21,5.4.4.2,5.4.99.5 0000162,0000287,0004106,0005886,0008909,0009058,0009697,0010106,0016829,0016833,0016853,0019540,0043904,0046872,0052572 Ki=12uM P9WFX1 22607697 451 ASSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTRRQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAIDRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQLGGIRALGYSPELVTAVRADGVVITEPLAGTARDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAVIDFMTVREQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVAR