2fl5 A 3.0 BS01 RBF B 1 G95 Y95A G94 Y95 ? Q5FWF9 16537445 202 SYELKQPPSVSVSPGQTARITCSGDVLPKKYAYWYQERSGQAPVLVVYEDSGRPSEIPERFSGSSSGTKATLTISGAQVEDEADYYCYSDISNGYPLFGGGTKLSVGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPIKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT 2fl5 B 3.0 BS01 RBF B 1 Y33 N50 F58 Y100A Y33 N50 F59 Y105 16537445 202 EVQLVESGGGLVQPGESLKLSCTASGFSLSNYYMTWVRQAPGKGLEWVTNIRPDETEKFYSDSVKGRFTVSRDNARNSLFNSMSLQRVEDTATYYCARVSDFGDYGPDFWGQGTLVSVTSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVNHKPSNTKVDKKVEP 2fl5 D 3.0 BS01 RBF D 1 Y33 N50 E56 F58 Y100A Y33 N50 E57 F59 Y105 16537445 228 EVQLVESGGGLVQPGESLKLSCTASGFSLSNYYMTWVRQAPGKGLEWVTNIRPDETEKFYSDSVKGRFTVSRDNARNSLFNSMSLQRVEDTATYYCARVSDFGDYGPDFWGQGTLVSVTSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVNHKPSNTKVDKKVEP 2fl5 F 3.0 BS01 RBF F 1 Y33 N50 R52 F58 Y100A Y33 N50 R52 F59 Y105 16537445 204 EVQLVESGGGLVQPGESLKLSCTASGFSLSNYYMTWVRQAPGKGLEWVTNIRPDETEKFYSDSVKGRFTVSRDNARNSLFNSMSLQRVEDTATYYCARVSDFGDYGPDFWGQGTLVSVTSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVNHKPSNTKVDKKVEP 2fl5 H 3.0 BS01 RBF H 1 Y33 N50 R52 E56 F58 Y100A Y33 N50 R52 E57 F59 Y105 -logKd/Ki=8.74, Kd=1.8nM 16537445 201 EVQLVESGGGLVQPGESLKLSCTASGFSLSNYYMTWVRQAPGKGLEWVTNIRPDETEKFYSDSVKGRFTVSRDNARNSLFNSMSLQRVEDTATYYCARVSDFGDYGPDFWGQGTLVSVTSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVNHKPSNTKVDKKVEP 2fl5 L 3.0 BS01 RBF H 1 G95 Y95A G94 Y95 ? -logKd/Ki=8.74, Kd=1.8nM Q5FWF9 16537445 201 SYELKQPPSVSVSPGQTARITCSGDVLPKKYAYWYQERSGQAPVLVVYEDSGRPSEIPERFSGSSSGTKATLTISGAQVEDEADYYCYSDISNGYPLFGGGTKLSVGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPIKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPT