2d06 A 2.3 BS01 A3P A 1 K48 S49 T51 T52 W53 R130 S138 Y193 F255 M256 R257 K258 G259 K41 S42 T44 T45 W46 R123 S131 Y186 F248 M249 R250 K251 G252 K48 H108 S138 K41 H101 S131 2.8.2.1 0004062,0005515,0005737,0005829,0006068,0006584,0006805,0008146,0008202,0008210,0009308,0009812,0016740,0047894,0050294,0050427,0050656,0051923 P50225 16221673 2001 SRPPLEYVKGVPLIKYFAEALGPLQSFQARPDDLLISTYPKSGTTWVSQILDMIYQGGDLEKCHRAPIFMRVPFLEFKAPGIPSGMETLKDTPAPRLLKTHLPLALLPQTLLDQKVKVVYVARNAKDVAVSYYHFYHMAKVHPEPGTWDSFLEKFMVGEVSYGSWYQHVQEWWELSRTHPVLYLFYEDMKENPKREIQKILEFVGHSLPEETVDFMVQHTSFKEMKKNPMTNYTTVPQEFMDHSISPFMRKGMAGDWKTTFTVAQNERFDADYAEKMAGCSLSFRSEL 2d06 A 2.3 BS02 EST A 1 P47 K106 H108 F142 V148 F247 P40 K99 H101 F135 V141 F240 K48 H108 S138 K41 H101 S131 2.8.2.1 0004062,0005515,0005737,0005829,0006068,0006584,0006805,0008146,0008202,0008210,0009308,0009812,0016740,0047894,0050294,0050427,0050656,0051923 Ki=83.2uM -logKd/Ki=4.08, Ki=83.2uM Kd=1200nM P50225 16221673 304 SRPPLEYVKGVPLIKYFAEALGPLQSFQARPDDLLISTYPKSGTTWVSQILDMIYQGGDLEKCHRAPIFMRVPFLEFKAPGIPSGMETLKDTPAPRLLKTHLPLALLPQTLLDQKVKVVYVARNAKDVAVSYYHFYHMAKVHPEPGTWDSFLEKFMVGEVSYGSWYQHVQEWWELSRTHPVLYLFYEDMKENPKREIQKILEFVGHSLPEETVDFMVQHTSFKEMKKNPMTNYTTVPQEFMDHSISPFMRKGMAGDWKTTFTVAQNERFDADYAEKMAGCSLSFRSEL 2d06 B 2.3 BS01 A3P B 1 K48 G50 T51 T52 W53 R130 S138 Y193 F229 F255 M256 R257 G259 K41 G43 T44 T45 W46 R123 S131 Y186 F222 F248 M249 R250 G252 K48 H108 S138 K41 H101 S131 2.8.2.1 0004062,0005515,0005737,0005829,0006068,0006584,0006805,0008146,0008202,0008210,0009308,0009812,0016740,0047894,0050294,0050427,0050656,0051923 P50225 16221673 2002 SRPPLEYVKGVPLIKYFAEALGPLQSFQARPDDLLISTYPKSGTTWVSQILDMIYQGGDLEKCHRAPIFMRVPFLEFKAPGIPSGMETLKDTPAPRLLKTHLPLALLPQTLLDQKVKVVYVARNAKDVAVSYYHFYHMAKVHPEPGTWDSFLEKFMVGEVSYGSWYQHVQEWWELSRTHPVLYLFYEDMKENPKREIQKILEFVGHSLPEETVDFMVQHTSFKEMKKNPMTNYTTVPQEFMDHSISPFMRKGMAGDWKTTFTVAQNERFDADYAEKMAGCSLSFRSEL 2d06 B 2.3 BS02 EST B 1 F24 P47 K106 H108 F142 Y240 F247 F17 P40 K99 H101 F135 Y233 F240 K48 H108 S138 K41 H101 S131 2.8.2.1 0004062,0005515,0005737,0005829,0006068,0006584,0006805,0008146,0008202,0008210,0009308,0009812,0016740,0047894,0050294,0050427,0050656,0051923 Ki=83.2uM Kd=1200nM P50225 16221673 305 SRPPLEYVKGVPLIKYFAEALGPLQSFQARPDDLLISTYPKSGTTWVSQILDMIYQGGDLEKCHRAPIFMRVPFLEFKAPGIPSGMETLKDTPAPRLLKTHLPLALLPQTLLDQKVKVVYVARNAKDVAVSYYHFYHMAKVHPEPGTWDSFLEKFMVGEVSYGSWYQHVQEWWELSRTHPVLYLFYEDMKENPKREIQKILEFVGHSLPEETVDFMVQHTSFKEMKKNPMTNYTTVPQEFMDHSISPFMRKGMAGDWKTTFTVAQNERFDADYAEKMAGCSLSFRSEL