1m1b A 2.25 BS02 SPV A 1 S46 G47 L48 D85 N122 S123 L124 R159 S42 G43 L44 D81 N118 S119 L120 R155 W44 S46 G47 L48 D58 D85 D87 C112 E114 K120 N122 S123 R159 H190 V215 W40 S42 G43 L44 D54 D81 D83 C108 E110 K116 N118 S119 R155 H186 V211 5.4.2.9 0003824,0016853,0032923,0046872,0050188 Ki=22uM -logKd/Ki=4.66, Ki=22uM P56839 12162742 996 VKKTTQLKQMLNSKDLEFIMEAHNGLSARIVQEAGFKGIWGSGLSVSAQLGVRDSNEASWTQVVEVLEFMSDASDVPILLDADTGYGNFNNARRLVRKLEDRGVAGACLEDKLFPKTNSLHDGRAQPLADIEEFALKIKACKDSQTDPDFCIVARVEAFIAGWGLDEALKRAEAYRNAGADAILMHSKKADPSDIEAFMKAWNNQGPVVIVPTKYYKTPTDHFRDMGVSMVIWANHNLRASVSAIQQTTKQIYDDQSLVNVEDKIVSVKEIFRLQRDDELVQAEDKYLPKN 1m1b B 2.25 BS01 SPV B 1 S46 L48 D85 L124 R159 H190 S42 L44 D81 L120 R155 H186 W44 S46 G47 L48 D58 D85 D87 C112 E114 K120 N122 S123 R159 H190 V215 W40 S42 G43 L44 D54 D81 D83 C108 E110 K116 N118 S119 R155 H186 V211 5.4.2.9 0003824,0016853,0032923,0046872,0050188 Ki=22uM P56839 12162742 997 VKKTTQLKQMLNSKDLEFIMEAHNGLSARIVQEAGFKGIWGSGLSVSAQLGVRDSNEASWTQVVEVLEFMSDASDVPILLDADTGYGNFNNARRLVRKLEDRGVAGACLEDKLFPKTNSLHDGRAQPLADIEEFALKIKACKDSQTDPDFCIVARVEAFIAGWGLDEALKRAEAYRNAGADAILMHSKKADPSDIEAFMKAWNNQGPVVIVPTKYYKTPTDHFRDMGVSMVIWANHNLRASVSAIQQTTKQIYDDQSLVNVEDKIVSVKEIFRLQRDDELVQAEDKYLP 3dt7 A 1.5 BS03 SPV A 1 R87 G237 K243 K244 H264 S286 F333 R405 R89 G239 K245 K246 H266 S288 F335 R407 R87 Y235 K244 H264 S286 C288 D311 R405 R89 Y237 K246 H266 S288 C290 D313 R407 2.7.11.-,4.1.1.32 0000287,0004550,0004611,0004613,0005525,0005737,0005783,0005829,0006006,0006094,0006107,0006629,0009617,0014823,0016301,0016310,0016831,0017076,0018105,0019003,0019543,0030145,0031406,0031667,0032496,0032868,0032869,0033993,0042593,0042594,0043382,0043648,0045944,0046166,0046327,0046872,0046889,0046890,0051365,0070365,0070741,0071300,0071320,0071332,0071333,0071347,0071356,0071377,0071456,0071474,0071475,0071476,0071477,0071549,0072350,0097403,0106264,1904628,1904640 P07379 18772387 900 GSMPPQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 3dt7 B 1.5 BS03 SPV B 1 R87 G237 K243 K244 H264 S286 R405 R84 G234 K240 K241 H261 S283 R402 R87 Y235 K244 H264 S286 C288 D311 R405 R84 Y232 K241 H261 S283 C285 D308 R402 2.7.11.-,4.1.1.32 0000287,0004550,0004611,0004613,0005525,0005737,0005783,0005829,0006006,0006094,0006107,0006629,0009617,0014823,0016301,0016310,0016831,0017076,0018105,0019003,0019543,0030145,0031406,0031667,0032496,0032868,0032869,0033993,0042593,0042594,0043382,0043648,0045944,0046166,0046327,0046872,0046889,0046890,0051365,0070365,0070741,0071300,0071320,0071332,0071333,0071347,0071356,0071377,0071456,0071474,0071475,0071476,0071477,0071549,0072350,0097403,0106264,1904628,1904640 P07379 18772387 900 QLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEAKVIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 3moe A 1.25 BS03 SPV A 1 R87 G237 K243 K244 H264 S286 R405 R89 G239 K245 K246 H266 S288 R407 R87 Y235 K244 H264 S286 C288 D311 R405 R89 Y237 K246 H266 S288 C290 D313 R407 2.7.11.-,4.1.1.32 0000287,0004550,0004611,0004613,0005525,0005737,0005783,0005829,0006006,0006094,0006107,0006629,0009617,0014823,0016301,0016310,0016831,0017076,0018105,0019003,0019543,0030145,0031406,0031667,0032496,0032868,0032869,0033993,0042593,0042594,0043382,0043648,0045944,0046166,0046327,0046872,0046889,0046890,0051365,0070365,0070741,0071300,0071320,0071332,0071333,0071347,0071356,0071377,0071456,0071474,0071475,0071476,0071477,0071549,0072350,0097403,0106264,1904628,1904640 Ki=50uM -logKd/Ki=4.30, Ki=50uM P07379 20476774 1200 GSMPPQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATGKVIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 4gmm A 1.736 BS03 SPV A 1 R87 G236 G237 K243 K244 H264 S286 R405 R85 G234 G235 K241 K242 H262 S284 R403 R87 Y235 K244 H264 S286 C288 D311 R405 R85 Y233 K242 H262 S284 C286 D309 R403 2.7.11.-,4.1.1.32 0004611,0004613,0005525,0006094,0017076 P07379 23127136 705 PQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEGIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 4gmz A 2.05 BS03 SPV A 1 R87 G237 K243 K244 H264 S286 R405 R86 G236 K242 K243 H263 S285 R404 R87 Y235 K244 H264 S286 C288 D311 R405 R86 Y234 K243 H263 S285 C287 D310 R404 2.7.11.-,4.1.1.32 0004611,0004613,0005525,0006094,0017076 P07379 23127136 705 PPQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEGGIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 4gno A 1.5 BS03 SPV A 1 R87 G237 K243 K244 H264 S286 R405 R86 G236 K242 K243 H263 S285 R404 R87 Y235 K244 H264 S286 C288 D311 R405 R86 Y234 K243 H263 S285 C287 D310 R404 2.7.11.-,4.1.1.32 0004611,0004613,0005525,0006094,0017076 P07379 23127136 705 PPQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEGGGIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 4ox2 A 2.0 BS03 SPV A 1 R87 G237 K243 K244 H264 S286 D311 R405 R85 G235 K241 K242 H262 S284 D309 R400 R87 Y235 K244 H264 S286 C288 D311 R405 R85 Y233 K242 H262 S284 C286 D309 R400 2.7.11.-,4.1.1.32 0000287,0004550,0004611,0004613,0005525,0005737,0005783,0005829,0006006,0006094,0006107,0006629,0009617,0014823,0016301,0016310,0016831,0017076,0018105,0019003,0019543,0030145,0031406,0031667,0032496,0032868,0032869,0033993,0042593,0042594,0043382,0043648,0045944,0046166,0046327,0046872,0046889,0046890,0051365,0070365,0070741,0071300,0071320,0071332,0071333,0071347,0071356,0071377,0071456,0071474,0071475,0071476,0071477,0071549,0072350,0097403,0106264,1904628,1904640 P07379 24863970 704 PQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHTCDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEAVIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 4ox2 B 2.0 BS03 SPV B 1 R87 G237 K243 K244 H264 S286 F333 R405 R85 G235 K241 K242 H262 S284 F331 R403 R87 Y235 K244 H264 S286 C288 D311 R405 R85 Y233 K242 H262 S284 C286 D309 R403 2.7.11.-,4.1.1.32 0000287,0004550,0004611,0004613,0005525,0005737,0005783,0005829,0006006,0006094,0006107,0006629,0009617,0014823,0016301,0016310,0016831,0017076,0018105,0019003,0019543,0030145,0031406,0031667,0032496,0032868,0032869,0033993,0042593,0042594,0043382,0043648,0045944,0046166,0046327,0046872,0046889,0046890,0051365,0070365,0070741,0071300,0071320,0071332,0071333,0071347,0071356,0071377,0071456,0071474,0071475,0071476,0071477,0071549,0072350,0097403,0106264,1904628,1904640 P07379 24863970 704 PQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHTCDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 5fh4 A 1.49 BS04 SPV A 1 R87 G237 K243 K244 H264 S286 R405 R84 G234 K240 K241 H261 S283 R402 R87 Y235 K244 H264 S286 C288 D311 R405 R84 Y232 K241 H261 S283 C285 D308 R402 2.7.11.-,4.1.1.32 0000287,0004550,0004611,0004613,0005525,0005737,0005783,0005829,0006006,0006094,0006107,0006629,0009617,0014823,0016301,0016310,0016831,0017076,0018105,0019003,0019543,0030145,0031406,0031667,0032496,0032868,0032869,0033993,0042593,0042594,0043382,0043648,0045944,0046166,0046327,0046872,0046889,0046890,0051365,0070365,0070741,0071300,0071320,0071332,0071333,0071347,0071356,0071377,0071456,0071474,0071475,0071476,0071477,0071549,0072350,0097403,0106264,1904628,1904640 Ki=174uM P07379 26709450 705 QLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIDSKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEAGKVIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 5v9f A 2.05 BS03 SPV A 1 R87 G236 G237 K243 K244 H264 S286 R405 R85 G234 G235 K241 K242 H262 S284 R403 R87 Y235 K244 H264 S286 C288 D311 R405 R85 Y233 K242 H262 S284 C286 D309 R403 2.7.11.-,4.1.1.32 0000287,0004550,0004611,0004613,0005525,0005737,0005783,0005829,0006006,0006094,0006107,0006629,0009617,0014823,0016301,0016310,0016831,0017076,0018105,0019003,0019543,0030145,0031406,0031667,0032496,0032868,0032869,0033993,0042593,0042594,0043382,0043648,0045944,0046166,0046327,0046872,0046889,0046890,0051365,0070365,0070741,0071300,0071320,0071332,0071333,0071347,0071356,0071377,0071456,0071474,0071475,0071476,0071477,0071549,0072350,0097403,0106264,1904628,1904640 Ki=37uM P07379 28345895 705 PQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEAVIMRDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM