1at1 A 2.8 BS02 PCT A 1 S52 T53 R54 T55 R105 H134 Q137 S52 T53 R54 T55 R105 H134 Q137 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 2405902 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 1at1 C 2.8 BS02 PCT C 1 S52 T53 R54 T55 R105 H134 L267 S52 T53 R54 T55 R105 H134 L267 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 2405902 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 1tu0 A 2.55 BS01 PCT A 1 S52 T53 R54 T55 R105 H134 Q137 S52 T53 R54 T55 R105 H134 Q137 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 15288791 1311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFAASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 1tu0 C 2.55 BS01 PCT C 1 S52 R54 T55 H134 S52 R54 T55 H134 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 15288791 1312 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFAASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 1tug A 2.1 BS02 PCT A 1 S52 T55 R105 H134 S52 T55 R105 H134 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 15288791 1314 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFAASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 1tug C 2.1 BS01 PCT C 1 S52 R54 T55 R105 S52 R54 T55 R105 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 15288791 1313 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFAASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 2a0f A 2.9 BS01 PCT A 1 R54 T55 H134 Q137 L267 R54 T55 H134 Q137 L267 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 16120448 1311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLAPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 2at1 A 2.8 BS02 PCT A 1 S52 T53 R54 T55 R105 H134 Q137 S52 T53 R54 T55 R105 H134 Q137 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 2405902 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 2at1 C 2.8 BS02 PCT C 1 S52 T53 R54 T55 R105 H134 L267 S52 T53 R54 T55 R105 H134 L267 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 2405902 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 2hse A 2.6 BS01 PCT A 1 S52 T53 R54 T55 R105 L267 S52 T53 R54 T55 R105 L267 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 811 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLAPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 2hse C 2.6 BS01 PCT C 1 S52 T53 R54 T55 R105 P266 L267 S52 T53 R54 T55 R105 P266 L267 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 911 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLAPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 3at1 A 2.8 BS01 PCT A 1 S52 T53 R54 T55 R105 S52 T53 R54 T55 R105 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 -logKd/Ki=3.18, Ki=0.66mM P0A786 2405902 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 3at1 C 2.8 BS01 PCT C 1 S52 R54 T55 S52 R54 T55 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 2405902 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 4mv6 A 1.77 BS01 PCT A 1 K238 R292 Q294 V295 E296 R338 K206 R260 Q262 V263 E264 R306 K116 H209 R235 T274 E276 E288 N290 R292 E296 R338 K116 H177 R203 T242 E244 E256 N258 R260 E264 R306 6.3.4.14 0003824,0003989,0004075,0005524,0006633,0016874,0046872,2001295 P43873 26020841 504 MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRDLKHVLLADETICIGPAPSAKSYLNIPAIIAAAEVTGADAIHPGYGFLSENADFAEQVERSGFTFIGPTADVIRLMGDKVSAIKAMKKAGVPCVPGSKEIAKRIGYPIIIRVVRSEDALEESIAMTKAVYMEKYLENPRHVEIQVLADTHGNAVYLAERDCSMQRRHQKVVEEAPAPGITEEVRRDIGSRCANACVEIGYRGAGTFEFLYENGEFYFIEMNTRIQVEHPVTEMITGVDLVKEQLRIAAGLPISFKQEDIKVKGHAMECRINAEDPKTFLPSPGKVNHLHSPGGLGVRWDSHVYGGYTVPPHYDSMIAKLITYGDTREVAIRRMQNALSETIIDGIKTNIPLHELILEDENFQKGGTNIHYLEKKL 7at1 A 2.8 BS02 PCT A 1 S52 T53 R54 T55 R105 H134 Q137 S52 T53 R54 T55 R105 H134 Q137 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 2271529 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 7at1 C 2.8 BS02 PCT C 1 S52 T53 R54 T55 R105 L267 S52 T53 R54 T55 R105 L267 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 2271529 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 8at1 A 2.8 BS02 PCT A 1 S52 T53 R54 T55 R105 Q137 P266 L267 S52 T53 R54 T55 R105 Q137 P266 L267 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 2271529 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL 8at1 C 2.8 BS02 PCT C 1 S52 T53 R54 T55 R105 P266 L267 S52 T53 R54 T55 R105 P266 L267 R54 T55 K84 R105 H134 Q137 T228 P266 G292 R54 T55 K84 R105 H134 Q137 T228 P266 G292 2.1.3.2 0004070,0004088,0005515,0005737,0005829,0006207,0006221,0006520,0006541,0009347,0016597,0016740,0016743,0042802,0044205,0070207 P0A786 2271529 311 ANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFQTSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQQTEGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPEYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL