2vw2 A 1.7 BS01 NHE A 1 R245 D270 D327 N352 Y489 R557 R619 Y653 W674 R207 D232 D289 N314 Y451 R519 R581 Y615 W636 D270 E541 Y653 D232 E503 Y615 3.2.1.18 0004308,0005975 -logKd/Ki=3.30, Ki=0.5mM Q54727 18835278 1000 ISPIFQGGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYFSIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMYVNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQEVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKINIATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDKKSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVYNETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSLFKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFATYTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYMTSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQLVVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLSNVVQYIDLEINDLT 2vw2 A 1.7 BS02 NHE A 2 K334 K499 E658 K296 K461 E620 D270 E541 Y653 D232 E503 Y615 3.2.1.18 0004308,0005975 -logKd/Ki=3.30, Ki=0.5mM Q54727 18835278 1001 ISPIFQGGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYFSIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMYVNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQEVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKINIATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDKKSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVYNETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSLFKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFATYTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYMTSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQLVVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLSNVVQYIDLEINDLT 3kjr A 1.95 BS02 NHE A 1 H36 F172 S173 D175 S266 R267 H32 F168 S169 D171 S253 R254 I29 D37 E285 W307 Y333 C393 R413 D416 I25 D33 E272 W294 Y320 C380 R400 D403 1.5.1.3,2.1.1.45 0000166,0004146,0004799,0005739,0005829,0006231,0006730,0008168,0009165,0016491,0016741,0032259,0046654 A7ASX7 20000709 513 YEGCGDLTIFVAVALNKVIGHKNQIPWPHITHDFRFLRNGTTYIPPEVLSKNPDIQNVVIFGRKTYESIPKASLPLKNRINVILSRTVKEVPGCLVYEDLSTAIRDLRANVPHNKIFILGGSFLYKEVLDNGLCDKIYLTRLNKEYPGDTYFPDIPDTFEITAISPTFSTDFVSYDFVIYERKPFDQLLMTGTDISVPKPKYVACPGVRIRNHEEFQYLDILADVLSHGVLKPNRTGTDAYSKFGYQMRFDLSRSFPLLTTKKVALRSIIEELLWFIKGSTNGNDLLAKNVRIWELNGRRDFLDKNGFTDREEHDLGPIYGFQWRHFGAEYLDMHADYTGKGIDQLAEIINRIKTNPNDRRLIVCSWNVSDLKKMALPPCHCFFQFYVSDNKLSCMMHQRSCDLGLGVPFNIASYSILTAMVAQVCGLGLGEFVHNLADAHIYVDHVDAVTTQIARIPHPFPRLRLNPDIRNIEDFTIDDIVVEDYVSHPPIPMAMSA 4q11 A 1.95 BS01 NHE A 1 P21 Y22 C37 L38 T99 R102 P15 Y16 C31 L32 T93 R96 ? 0005737,0045893 B4EUU9 25056110 201 PTRGKLLKRFAQIGPYIREQQCQESQFFFDCLAVCVNKKVTPEKREFWGWWMELERNGEQLIYYYQVGLFDKNGDWVNQVISKKDVIESIHETLIRFHDFLQAAVSELEMTLVPDEKMSNFPLPLK 4q11 B 1.95 BS01 NHE B 1 P21 Y22 C37 L38 R102 P17 Y18 C33 L34 R98 ? 0005737,0045893 B4EUU9 25056110 201 LHPTRGKLLKRFAQIGPYIREQQCQESQFFFDCLAVCVNKKVTPEKREFWGWWMELERNGEQLIYYYQVGLFDKNGDWVNQVISKKDVIESIHETLIRFHDFLQAAVSELEMTLVPDEKMSNFPLP 4ygr A 1.703 BS01 NHE A 1 D9 W58 W61 D9 W58 W61 D7 D9 E15 K38 T109 S110 G111 K141 G165 D166 L168 Q170 D171 D7 D9 E15 K38 T109 S110 G111 K141 G165 D166 L168 Q170 D171 ? 0016311,0016787,0016791,0044283,0046872 B6YTD6 25858319 301 MKAVLFDIDGTILTEEPLIMLFLPQVYDKLSRKLGISKDEARERFLSEILGRRDSYDWHDWNFFFKLFDLDLKYEELLERYPHKLQVYPDTIPTLEWLRDTGYKLGIVTSGPKYQRLKLKLTGLLDYFDVVITRDDVNAIKPEPKIFLYTIERLGVEPGEAVMVGDSLSQDVYGAKSVGMTAVWINRNGDRGYNMADYEIRTLYELRKILGGERV 4ygr A 1.703 BS02 NHE A 2 L50 R53 W58 L50 R53 W58 D7 D9 E15 K38 T109 S110 G111 K141 G165 D166 L168 Q170 D171 D7 D9 E15 K38 T109 S110 G111 K141 G165 D166 L168 Q170 D171 ? 0016311,0016787,0016791,0044283,0046872 B6YTD6 25858319 302 MKAVLFDIDGTILTEEPLIMLFLPQVYDKLSRKLGISKDEARERFLSEILGRRDSYDWHDWNFFFKLFDLDLKYEELLERYPHKLQVYPDTIPTLEWLRDTGYKLGIVTSGPKYQRLKLKLTGLLDYFDVVITRDDVNAIKPEPKIFLYTIERLGVEPGEAVMVGDSLSQDVYGAKSVGMTAVWINRNGDRGYNMADYEIRTLYELRKILGGERV 5tsp A 1.24 BS01 NHE A 1 R266 D291 D328 F353 R555 R615 Y655 R24 D49 D86 F111 R313 R373 Y413 A290 D291 E539 Y655 A48 D49 E297 Y413 3.2.1.18 0004308 A0A0H2YQR1 28315686 1001 VEGAVKTEPVDLFHPGFLNSSNYRIPALFKTKEGTLIASIDARRQGGADAPNNDIDTAVRRSEDGGKTWDEGQIIMDYPDKSSVIDTTLIQDDETGRIFLLVTHFPSKYGFWNAGLGSGFKNIDGKEYLCLYDSSGKEFTVRENVVYDKDGNKTEYTTNALGDLFRNGTKIDNINSSTAPLKAKGTSYINLVYSDDDGKTWSEPQNINFQVKKDWMKFLGIAPGRGIQIKNGEHKGRIVVPVYYTNEKGKQSSAVIYSDDSGKNWTIGESPNDNRKLENGKIINSKTLSDDAPQLTECQVVEMPNGQLKLFMRNLSGYLNIATSFDGGATWDETVEKDTNVLEPYCQLSVINYSQKIDGKDAVIFSNPNARSRSNGTVRIGLINQVGTYENGEPKYEFDWKYNKLVKPGYYAYSCLTELSNGNIGLLYEGTPSEEMSYIEMNLKYLES 5tsp B 1.24 BS01 NHE B 1 R266 D291 D328 F353 F460 R555 R615 Y655 R24 D49 D86 F111 F218 R313 R373 Y413 A290 D291 E539 Y655 A48 D49 E297 Y413 3.2.1.18 0004308 A0A0H2YQR1 28315686 1001 VEGAVKTEPVDLFHPGFLNSSNYRIPALFKTKEGTLIASIDARRQGGADAPNNDIDTAVRRSEDGGKTWDEGQIIMDYPDKSSVIDTTLIQDDETGRIFLLVTHFPSKYGFWNAGLGSGFKNIDGKEYLCLYDSSGKEFTVRENVVYDKDGNKTEYTTNALGDLFRNGTKIDNINSSTAPLKAKGTSYINLVYSDDDGKTWSEPQNINFQVKKDWMKFLGIAPGRGIQIKNGEHKGRIVVPVYYTNEKGKQSSAVIYSDDSGKNWTIGESPNDNRKLENGKIINSKTLSDDAPQLTECQVVEMPNGQLKLFMRNLSGYLNIATSFDGGATWDETVEKDTNVLEPYCQLSVINYSQKIDGKDAVIFSNPNARSRSNGTVRIGLINQVGTYENGEPKYEFDWKYNKLVKPGYYAYSCLTELSNGNIGLLYEGTPSEEMSYIEMNLKYLES 5whk A 2.5 BS01 NHE A 1 S40 G43 E72 S37 G40 E64 ? P55899 28878017 301 HLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGAWVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGPDNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLWKEPPSMRLKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHYCCIVQHAGLAQPLRVELESP 5whk A 2.5 BS02 NHE A 2 Y9 E64 D67 F154 R162 Y6 E56 D59 F146 R154 ? P55899 28878017 302 HLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGAWVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGPDNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLWKEPPSMRLKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHYCCIVQHAGLAQPLRVELESP 6cex A 2.57 BS01 NHE A 1 G135 S136 W153 G126 S127 W144 ? 0019031,0019064,0046789 -logKd/Ki=1.70, Kd>20mM Q91MA7 29610325 401 GATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQNETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGSTYPVLNVTMPNNDNFDKLYIWGVHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGLSSRISIYWTIVKPGDVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEK 6cex B 2.57 BS01 NHE F 1 Y94 E97 Y94 E97 ? 0019031,0019064,0046789 P03436 29610325 201 GLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTGRQLRENAEDMGNGCFKIYHKCDNACIESIRNGTYDHDVYRDEALNNRF 6cex C 2.57 BS01 NHE C 1 G135 S136 W153 T155 G127 S128 W145 T147 ? 0019031,0019064,0046789 Q91MA7 29610325 401 PGATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQNETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGSTYPVLNVTMPNNDNFDKLYIWGVHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGLSSRISIYWTIVKPGDVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVP 6cex D 2.57 BS01 NHE B 1 Y94 E97 A101 Y94 E97 A101 ? 0019031,0019064,0046789 P03436 29610325 201 GLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTGRQLRENAEDMGNGCFKIYHKCDNACIESIRNGTYDHDVYRDEALNNRF 6cex E 2.57 BS01 NHE E 1 G135 S136 W153 L226 G127 S128 W145 L218 ? 0019031,0019064,0046789 Q91MA7 29610325 401 PGATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQNETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGSTYPVLNVTMPNNDNFDKLYIWGVHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIGSRPWVRGLSSRISIYWTIVKPGDVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEK 6cf5 A 2.04 BS01 NHE A 1 L133A S136 S137 W153 Q226 L132 S135 S136 W152 Q225 ? 0019031,0019064,0046789 -logKd/Ki=1.70, Kd>20mM Q6DQ33 29610325 401 DPGDQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKKHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKANPVNDLCYPGDFNDYEELKHLLSRINHFEKIQIIPKSSWSSHEASLGVSSACPYQGKSSFFRNVVWLIKKNSTYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPRIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKGDSTIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNSP 6cf5 C 2.04 BS01 NHE C 1 L133A V135 S136 S137 W153 I155 L194 Q226 L131 V133 S134 S135 W151 I153 L192 Q224 ? 0019031,0019064,0046789 Q6DQ33 29610325 401 PGDQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKKHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKANPVNDLCYPGDFNDYEELKHLLSRINHFEKIQIIPKSSWSSHEASLGVSSACPYQGKSSFFRNVVWLIKKNSTYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPRIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKGDSTIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNSP 6cf5 E 2.04 BS01 NHE E 1 L133A S136 S137 W153 Q226 L131 S134 S135 W151 Q224 ? 0019031,0019064,0046789 Q6DQ33 29610325 401 PGDQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKKHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKANPVNDLCYPGDFNDYEELKHLLSRINHFEKIQIIPKSSWSSHEASLGVSSACPYQGKSSFFRNVVWLIKKNSTYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPRIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKGDSTIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNSP 6jxn A 1.97 BS02 NHE D 1 S106 F107 K112 M161 Y165 S107 F108 K113 M161 Y165 1.6.5.-,1.7.1.17 0000166,0009055,0010181,0016491,0016652,0016655 G9QLG5 32861785 302 HMAKVLYITAHPLDETQSYSMAVGKAFIDTYKEVNPNDEVIHIDLYKENIPQIDADVFSGWGKLQSGKGFEELTAEEKAKVGRLAELSDQFVAADKYVFVTPMWNFSFPPVMKAYIDSVAVAGKTFKYTEGSVGLLTDKKALHIQASGGIYSEGPAAEMEMGHRYLQAIMNFFGVPSFEGLFVEGQNAMPDKAQEIKEKAIARAKDLAHTF 6jxn B 1.97 BS02 NHE B 1 A119 F125 F172 A120 F126 F173 1.6.5.-,1.7.1.17 0000166,0009055,0010181,0016491,0016652,0016655 G9QLG5 32861785 302 HMAKVLYITAHPLDETQSYSMAVGKAFIDTYKEVNPNDEVIHIDLYKENIPQIDADVFSGWGKLQSGKGFEELTAEEKAKVGRLAELSDQFVAADKYVFVTPMWNFSFPPVMKAYIDSVAVAGKTFKYTEQGSVGLLTDKKALHIQASGGIYSEGPAAEMEMGHRYLQAIMNFFGVPSFEGLFVEGQNAMPDKAQEIKEKAIARAKDLAHTF 6jxn B 1.97 BS03 NHE B 2 S106 F107 K112 M161 Y165 S107 F108 K113 M162 Y166 1.6.5.-,1.7.1.17 0000166,0009055,0010181,0016491,0016652,0016655 G9QLG5 32861785 303 HMAKVLYITAHPLDETQSYSMAVGKAFIDTYKEVNPNDEVIHIDLYKENIPQIDADVFSGWGKLQSGKGFEELTAEEKAKVGRLAELSDQFVAADKYVFVTPMWNFSFPPVMKAYIDSVAVAGKTFKYTEQGSVGLLTDKKALHIQASGGIYSEGPAAEMEMGHRYLQAIMNFFGVPSFEGLFVEGQNAMPDKAQEIKEKAIARAKDLAHTF 6jxn C 1.97 BS01 NHE B 2 S106 M161 Y165 S107 M158 Y162 1.6.5.-,1.7.1.17 0000166,0009055,0010181,0016491,0016652,0016655 G9QLG5 32861785 303 HMAKVLYITAHPLDETQSYSMAVGKAFIDTYKEVNPNDEVIHIDLYKENIPQIDADVFSGWGKLQSGKGFEELTAEEKAKVGRLAELSDQFVAADKYVFVTPMWNFSFPPVMKAYIDSVAVAGKTFKYSVGLLTDKKALHIQASGGIYSEGPAAEMEMGHRYLQAIMNFFGVPSFEGLFVEGQNAMPDKAQEIKEKAIARAKDLAHTF 6jxn D 1.97 BS02 NHE D 1 S106 F107 K112 M161 Y165 S107 F108 K113 M162 Y166 1.6.5.-,1.7.1.17 0000166,0009055,0010181,0016491,0016652,0016655 G9QLG5 32861785 302 HMAKVLYITAHPLDETQSYSMAVGKAFIDTYKEVNPNDEVIHIDLYKENIPQIDADVFSGWGKLQSGKGFEELTAEEKAKVGRLAELSDQFVAADKYVFVTPMWNFSFPPVMKAYIDSVAVAGKTFKYTEQGSVGLLTDKKALHIQASGGIYSEGPAAEMEMGHRYLQAIMNFFGVPSFEGLFVEGQNAMPDKAQEIKEKAIARAKDLAHTF