3kc3 A 2.9 BS01 MK2 A 1 L70 V78 K93 L141 D142 L193 T206 D207 L28 V36 K51 L99 D100 L148 T161 D162 D186 K188 E190 N191 D207 D141 K143 E145 N146 D162 2.7.11.1 0004672,0005524,0006468 -logKd/Ki=6.80, IC50=0.16uM IC50=1300nM P49137 19919896 500 FPQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKE 3kc3 B 2.9 BS01 MK2 B 1 L70 V78 A91 K93 E139 L141 L193 T206 D207 L27 V35 A48 K50 E96 L98 L150 T163 D164 D186 K188 E190 N191 D207 D143 K145 E147 N148 D164 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 PQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERW 3kc3 C 2.9 BS01 MK2 C 1 L70 V78 K93 M138 E139 L141 L193 T206 D207 L30 V38 K53 M98 E99 L101 L150 T163 D164 D186 K188 E190 N191 D207 D143 K145 E147 N148 D164 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 QQFPQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKED 3kc3 D 2.9 BS01 MK2 D 1 V78 K93 L141 L193 T206 D207 V33 K48 L96 L142 T155 D156 D186 K188 E190 N191 D207 D135 K137 E139 N140 D156 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 FHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVL 3kc3 E 2.9 BS01 MK2 E 1 L70 V78 A91 K93 E139 L141 D142 L193 T206 D207 L27 V35 A48 K50 E96 L98 D99 L150 T163 D164 D186 K188 E190 N191 D207 D143 K145 E147 N148 D164 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 PQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKED 3kc3 F 2.9 BS01 MK2 F 1 L70 V78 K93 E139 L141 L193 T206 D207 L27 V35 K50 E96 L98 L150 T163 D164 D186 K188 E190 N191 D207 D143 K145 E147 N148 D164 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 PQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWE 3kc3 H 2.9 BS01 MK2 H 1 L70 V78 K93 M138 E139 L141 L193 T206 D207 L27 V35 K50 M95 E96 L98 L150 T163 D164 D186 K188 E190 N191 D207 D143 K145 E147 N148 D164 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 PQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTPYYVAPEVLGEKYDKSCDMWSLGVIMYILLCGYPPFYSNSPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWE 3kc3 I 2.9 BS01 MK2 I 1 L70 G73 V78 K93 L141 G144 L193 T206 D207 L26 G29 V34 K49 L97 G100 L149 T162 D163 D186 K188 E190 N191 D207 D142 K144 E146 N147 D163 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 QFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEM 3kc3 J 2.9 BS01 MK2 J 1 L70 V78 K93 L141 D142 L193 T206 D207 L28 V36 K51 L99 D100 L148 T161 D162 D186 K188 E190 N191 D207 D141 K143 E145 N146 D162 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 FPQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKER 3kc3 K 2.9 BS01 MK2 K 1 L70 G71 V78 A91 K93 L141 D142 G144 L193 T206 D207 L27 G28 V35 A48 K50 L98 D99 G101 L150 T163 D164 D186 K188 E190 N191 D207 D143 K145 E147 N148 D164 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 PQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKED 3kc3 L 2.9 BS01 MK2 L 1 L70 V78 K93 M138 L141 G144 L193 T206 D207 L29 V37 K52 M97 L100 G103 L152 T165 D166 D186 K188 E190 N191 D207 D145 K147 E149 N150 D166 2.7.11.1 0004672,0005524,0006468 IC50=1300nM P49137 19919896 500 QFPQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKER