1oar H 2.23 BS01 AZN H 1 W33 R50 K59 M99 Y105 W33 R50 K59 M99 Y105 Kd=40nM -logKd/Ki=7.40, Kd=40nM 12610298 500 EVQLQQSGAELVKPGASVKLSCKASGYTFTSYWMHWVKQRPGRGLEWIGRIDPNGGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDSAVYYCARMWYYGTYYFDYWGQGTTLTVSSAA 1oar I 2.23 BS01 AZN I 1 W33 R50 K59 M99 Y105 W20 R33 K38 M71 Y77 Kd=40nM 12610298 500 PGASVKLSCKASGYTFTSYWMHWVKQRLEWIGRIDGTKYNEKFKSKATLTVSTAYMQLSSLDSAVYYCARMWYYGTYYFDYWGQGTTL 1oar J 2.23 BS01 AZN J 1 W33 R50 K59 M99 Y105 W33 R50 K59 M99 Y105 Kd=40nM 12610298 500 EVQLQQSGAELVKPGASVKLSCKASGYTFTSYWMHWVKQRPGRGLEWIGRIDPNGGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDSAVYYCARMWYYGTYYFDYWGQGTTLTVSSAA 1oar K 2.23 BS01 AZN K 1 W33 R50 K59 M99 Y105 W14 R27 K32 M65 Y71 Kd=40nM 12610298 500 PGASVKLSCKTSYWMHWVKQRLEWIGRIDGTKYNEKFKSKATLTVSTAYMQLSSLDSAVYYCARMWYYGTYYFDYWGQGTTL 1oar L 2.23 BS01 AZN H 1 Y34 N36 W93 Y33 N35 W92 ? 0002250,0005615,0006955,0019814 -logKd/Ki=7.40, Kd=40nM P01724 12610298 500 AVVTQESALTTSPGETVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGNKAALTITGAQTEDEAIYFCALWYSNHLVFGGGTKLTVL 1oar M 2.23 BS01 AZN I 1 Y34 N36 W93 Y28 N30 W87 ? 0002250,0005615,0006955,0019814 P01724 12610298 500 VVTQESALTTSPGETVTLTCRAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGNKAALTITGAQTEDEAIYFCALWYSNHLVFGGGTKLTVL 1oar N 2.23 BS01 AZN J 1 Y34 N36 W93 Y33 N35 W92 ? 0002250,0005615,0006955,0019814 P01724 12610298 500 AVVTQESALTTSPGETVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGNKAALTITGAQTEDEAIYFCALWYSNHLVFGGGTKLTVL 1oar O 2.23 BS01 AZN K 1 Y34 N36 W93 Y31 N33 W90 ? 0002250,0005615,0006955,0019814 P01724 12610298 500 VVTQESALTTSPGETVTLTCRSSTAVTTSNYANWVQEKPDHLFTGLIGGTNNRAPGVPARFSGSLIGNKAALTITGAQTEDEAIYFCALWYSNHLVFGGGTKLTVL 5scl A 2.134 BS03 AZN A 1 P65 H90 G91 Y95 K379 P40 H65 G66 Y70 K256 2.7.1.40 0000287,0003824,0004743,0006096,0030955 P30613 35290845 603 QQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPGSGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS 5scl B 2.134 BS03 AZN B 1 P65 N87 H90 G91 Y95 S374 G375 A378 K379 P56 N78 H81 G82 Y86 S267 G268 A271 K272 2.7.1.40 0000287,0003824,0004743,0006096,0030955 P30613 35290845 603 RADVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPGSGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS 5scl C 2.134 BS03 AZN C 1 P65 N87 H90 G91 Y95 S374 G375 A378 K379 P47 N69 H72 G73 Y77 S255 G256 A259 K260 2.7.1.40 0000287,0003824,0004743,0006096,0030955 P30613 35290845 603 ELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS 5scl E 2.134 BS03 AZN E 1 P65 N87 H90 Y95 S374 G375 A378 K379 P43 N65 H68 Y73 S250 G251 A254 K255 2.7.1.40 0000287,0003824,0004743,0006096,0030955 P30613 35290845 603 AFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS 5scl F 2.134 BS03 AZN F 1 P65 N87 H90 G91 Y95 S374 G375 A378 K379 P55 N77 H80 G81 Y85 S264 G265 A268 K269 2.7.1.40 0000287,0003824,0004743,0006096,0030955 P30613 35290845 603 ADVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS 5scl G 2.134 BS03 AZN G 1 P65 N87 H90 G91 Y95 S374 G375 K379 P45 N67 H70 G71 Y75 S254 G255 K259 2.7.1.40 0000287,0003824,0004743,0006096,0030955 P30613 35290845 603 GTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS 5y2u B 1.98 BS01 AZN B 1 F22 R90 L95 W115 R116 F20 R88 L93 W113 R114 H11 R62 E89 H186 H9 R60 E87 H184 5.4.2.11,5.4.2.4 0003824,0004082,0004619,0005515,0005576,0005737,0005829,0006094,0006096,0016020,0016787,0016853,0016868,0019901,0034774,0046538,0061621,0070062,1904813 IC50=2300nM P18669 302 AYKLVLIRHGESAWNLENRFSGWYDADLSPAGHEEAKRGGQALRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVYELDKNLKPIKPMQFLGDEETV 5y2u C 1.98 BS01 AZN C 1 F22 W115 R116 F21 W114 R115 H11 R62 E89 H186 H10 R61 E88 H185 5.4.2.11,5.4.2.4 0003824,0004082,0004619,0005515,0005576,0005737,0005829,0006094,0006096,0016020,0016787,0016853,0016868,0019901,0034774,0046538,0061621,0070062,1904813 IC50=2300nM P18669 301 AAYKLVLIRHGESAWNLENRFSGWYDADLSPAGHEEAKRGGQALRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVYELDKNLKPIKPMQFLGD 7u8y A 2.22 BS01 AZN A 1 A21 G23 L24 S78 G80 A81 Y129 A16 G18 L19 S73 G75 A76 Y124 3.1.11.2 0003676,0008408 Q91XB0 301 LPHGHMQTLIFLDLEATGLPSSRPEVTELCLLAVHRRALENTSISQGHPPPVPRPPRVVDKLSLCIAPGKACSPGASEITGLSKAELEVQGRQRFDDNLAILLRAFLQRQPQPCCLVAHNGDRYDFPLLQTELARLSTPSPLDGTFCVDSIAALKALEQASSRKSYSLGSIYTRLYWQAPTDSHTAEGDVLTLLSICQWKPQALLQWVDEHARPFSTVKPMYG 7u8y A 2.22 BS02 AZN A 2 D18 E20 I84 H195 D13 E15 I79 H184 3.1.11.2 0003676,0008408 Q91XB0 302 LPHGHMQTLIFLDLEATGLPSSRPEVTELCLLAVHRRALENTSISQGHPPPVPRPPRVVDKLSLCIAPGKACSPGASEITGLSKAELEVQGRQRFDDNLAILLRAFLQRQPQPCCLVAHNGDRYDFPLLQTELARLSTPSPLDGTFCVDSIAALKALEQASSRKSYSLGSIYTRLYWQAPTDSHTAEGDVLTLLSICQWKPQALLQWVDEHARPFSTVKPMYG 7u8y B 2.22 BS01 AZN B 1 D18 E20 G80 I84 H195 D200 D13 E15 G75 I79 H183 D188 3.1.11.2 0003676,0008408 Q91XB0 301 LPHGHMQTLIFLDLEATGLPSSRPEVTELCLLAVHRRALENTSISQGHPPPVPRPPRVVDKLSLCIAPGKACSPGASEITGLSKAELEVQGRQRFDDNLAILLRAFLQRQPQPCCLVAHNGDRYDFPLLQTELARLSTPSPLDGTFCVDSIAALKALEQASRKSYSLGSIYTRLYWQAPTDSHTAEGDVLTLLSICQWKPQALLQWVDEHARPFSTVKPMYG 7u8y B 2.22 BS02 AZN B 2 A21 G23 L24 S78 G80 A81 Y129 A16 G18 L19 S73 G75 A76 Y124 3.1.11.2 0003676,0008408 Q91XB0 302 LPHGHMQTLIFLDLEATGLPSSRPEVTELCLLAVHRRALENTSISQGHPPPVPRPPRVVDKLSLCIAPGKACSPGASEITGLSKAELEVQGRQRFDDNLAILLRAFLQRQPQPCCLVAHNGDRYDFPLLQTELARLSTPSPLDGTFCVDSIAALKALEQASRKSYSLGSIYTRLYWQAPTDSHTAEGDVLTLLSICQWKPQALLQWVDEHARPFSTVKPMYG