1sa4 A 2.1 BS02 FPP B 1 K164 Y166 K110 Y112 K164 K110 2.5.1.58,2.5.1.59 0004659,0004660,0004661,0004662,0004663,0005515,0005737,0005829,0005875,0005886,0005953,0005965,0007167,0007179,0007528,0008017,0008318,0018342,0018343,0018344,0030971,0035022,0043014,0060090,0071340,0090044,0090045,1904395 Kd=2.0nM P49354 15170324 440 FVSLDSPSYVLYRDRAEWADIDPVPQNDGPNPVVQIIYSDKFRDVYDYFRAVLQRDERSERAFKLTRDAIELNAANYTVWHFRRVLLKSLQKDLHEEMNYITAIIEEQPKNYQVWHHRRVLVEWLRDPSQELEFIADILNQDAKNYHAWQHRQWVIQEFKLWDNELQYVDQLLKEDVRNNSVWNQRYFVISNTTGYNDRAVLEREVQYTLEMIKLVPHNESAWNYLKGILQDRGLSKYPNLLNQLLDLQPSHSSPYLIAFLVDIYEDMLENQCDNKEDILNKALELCEILAKEKDTIRKEYWRYIGRSLQSKHST 1sa4 B 2.1 BS01 GLC C 1 Q233 N234 W235 Q219 N220 W221 H248 R291 K294 D297 C299 Y300 D352 D359 H362 H234 R277 K280 D283 C285 Y286 D338 D345 H348 2.5.1.58 0003824,0004659,0004660,0005515,0005829,0005875,0005965,0006629,0008270,0008318,0018343,0046872 P49356 15170324 1 SSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFSSYKFNHLVPRLVLQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQIVATDVCQFLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREKLLQYLYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIGGVPGMEAHGGYTFCGLAALVILKRERSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCYSFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGKSRDFYHTCYCLSGLSIAQHFGSGAMLHDVVLGVPENALQPTHPVYNIGPDKVIQATTYFLQKPVPGFE 1sa4 B 2.1 BS02 FRU C 1 Q233 N234 G237 N269 S272 Q219 N220 G223 N255 S258 H248 R291 K294 D297 C299 Y300 D352 D359 H362 H234 R277 K280 D283 C285 Y286 D338 D345 H348 2.5.1.58 0003824,0004659,0004660,0005515,0005829,0005875,0005965,0006629,0008270,0008318,0018343,0046872 P49356 15170324 2 SSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFSSYKFNHLVPRLVLQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQIVATDVCQFLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREKLLQYLYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIGGVPGMEAHGGYTFCGLAALVILKRERSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCYSFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGKSRDFYHTCYCLSGLSIAQHFGSGAMLHDVVLGVPENALQPTHPVYNIGPDKVIQATTYFLQKPVPGFE 1sa4 B 2.1 BS03 ZN B 1 D297 C299 H362 D283 C285 H348 H248 R291 K294 D297 C299 Y300 D352 D359 H362 H234 R277 K280 D283 C285 Y286 D338 D345 H348 2.5.1.58 0003824,0004659,0004660,0005515,0005829,0005875,0005965,0006629,0008270,0008318,0018343,0046872 P49356 15170324 439 SSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFSSYKFNHLVPRLVLQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQIVATDVCQFLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREKLLQYLYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIGGVPGMEAHGGYTFCGLAALVILKRERSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCYSFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGKSRDFYHTCYCLSGLSIAQHFGSGAMLHDVVLGVPENALQPTHPVYNIGPDKVIQATTYFLQKPVPGFE 1sa4 B 2.1 BS04 FPP B 1 R202 Y205 H248 G250 C254 R291 K294 Y300 W303 R188 Y191 H234 G236 C240 R277 K280 Y286 W289 H248 R291 K294 D297 C299 Y300 D352 D359 H362 H234 R277 K280 D283 C285 Y286 D338 D345 H348 2.5.1.58 0003824,0004659,0004660,0005515,0005829,0005875,0005965,0006629,0008270,0008318,0018343,0046872 P49356 15170324 440 SSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFSSYKFNHLVPRLVLQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQIVATDVCQFLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREKLLQYLYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIGGVPGMEAHGGYTFCGLAALVILKRERSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCYSFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGKSRDFYHTCYCLSGLSIAQHFGSGAMLHDVVLGVPENALQPTHPVYNIGPDKVIQATTYFLQKPVPGFE 1sa4 B 2.1 BS05 JAN B 1 W106 D297 C299 D359 Y361 H362 W92 D283 C285 D345 Y347 H348 H248 R291 K294 D297 C299 Y300 D352 D359 H362 H234 R277 K280 D283 C285 Y286 D338 D345 H348 2.5.1.58 0003824,0004659,0004660,0005515,0005829,0005875,0005965,0006629,0008270,0008318,0018343,0046872 IC50=0.860nM P49356 15170324 441 SSPVWSEPLYSLRPEHARERLQDDSVETVTSIEQAKVEEKIQEVFSSYKFNHLVPRLVLQREKHFHYLKRGLRQLTDAYECLDASRPWLCYWILHSLELLDEPIPQIVATDVCQFLELCQSPEGGFGGGPGQYPHLAPTYAAVNALCIIGTEEAYDIINREKLLQYLYSLKQPDGSFLMHVGGEVDVRSAYCAASVASLTNIITPDLFEGTAEWIARCQNWEGGIGGVPGMEAHGGYTFCGLAALVILKRERSLNLKSLLQWVTSRQMRFEGGFQGRCNKLVDGCYSFWQAGLLPLLHRALHAQGDPALSMSHWMFHQQALQEYILMCCQCPAGGLLDKPGKSRDFYHTCYCLSGLSIAQHFGSGAMLHDVVLGVPENALQPTHPVYNIGPDKVIQATTYFLQKPVPGFE