5hv1 A 3.103 BS01 ANP A 1 K22 R117 F130 A131 G132 T136 L138 G216 E217 V220 E297 Q309 R311 K22 R117 F130 A131 G132 T136 L138 G216 E217 V220 E297 Q309 R311 0005524,0016301,0016310,0016772 27001859 901 MKPYVLKFQEIRPHSEALVGGKGMNLGACSNIEGVHVPAGFCLTTEAYKRTLAENNEFTQLLQRLSSLKTSDMDAIREISETIRTLIQHTQIPSEIASYMDATLLDVGGYEMPFAVRSSATAEDLPHASFAGQHDTYLNIIGKDALLQHISMCWASLFTERAIIYRIQNQFDHRKVQLAVVIQQMISPEASGILFTADPITSNRKSLSIDASFGLGEALVSGLVSADSYTVRENTITNKIIATKKLAIYSLKEGGTETRILEKSQQTKQTLTDQQIIQLAKLGRKIEAYFGKPQDIEWCLAEGAFYIVQSRPITTLYPIPEVNEPGNRVYISVAHQQMMTDAMKPLGLSFYLMTTPATMYTAGGRLFVDITQSLSAKVSRDMMVNSLGQSDPLIKDALLTVINKKGFLPSIPDSSSVFELVRNSENSIKHLKQSIETKSGSDLFDFIVEDLEELKRVLFNPTSIDAIMAGMDASAWLNEHIYQWLGEKNVADKLSESAPNNITSQMGLELLDVADVIRPYPAVRAYLEQTKNPDFMNELATLEGGAETKKALEDYLQKYGMRCAGEIDLTKTRWIENPLTLIPLILSNIKNFDSSASMHKFAQGEKEAFHKEQEILRRLQELPDGEQKAMETKEKIDILRHFIGYREYPKYGMINRYFIYKLALLRAGEQLVKDGILQEHEDIYFLYFEELREVVRTGQVDYELINARKRDFATFEKLTPPRILTSDGEMINGEYKRENLPKDAILGLPVSSGTVEGRARVILEMEKADLEDGDILVTAYTDPSWTPAFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATTIIKDGQQIRINGTEGYIEILD 5hv1 A 3.103 BS02 RFP A 1 Y351 T355 P356 V368 F479 I487 Y351 T355 P356 V368 F459 I467 0005524,0016301,0016310,0016772 -logKd/Ki=4.10, Kd=79.4uM 27001859 902 MKPYVLKFQEIRPHSEALVGGKGMNLGACSNIEGVHVPAGFCLTTEAYKRTLAENNEFTQLLQRLSSLKTSDMDAIREISETIRTLIQHTQIPSEIASYMDATLLDVGGYEMPFAVRSSATAEDLPHASFAGQHDTYLNIIGKDALLQHISMCWASLFTERAIIYRIQNQFDHRKVQLAVVIQQMISPEASGILFTADPITSNRKSLSIDASFGLGEALVSGLVSADSYTVRENTITNKIIATKKLAIYSLKEGGTETRILEKSQQTKQTLTDQQIIQLAKLGRKIEAYFGKPQDIEWCLAEGAFYIVQSRPITTLYPIPEVNEPGNRVYISVAHQQMMTDAMKPLGLSFYLMTTPATMYTAGGRLFVDITQSLSAKVSRDMMVNSLGQSDPLIKDALLTVINKKGFLPSIPDSSSVFELVRNSENSIKHLKQSIETKSGSDLFDFIVEDLEELKRVLFNPTSIDAIMAGMDASAWLNEHIYQWLGEKNVADKLSESAPNNITSQMGLELLDVADVIRPYPAVRAYLEQTKNPDFMNELATLEGGAETKKALEDYLQKYGMRCAGEIDLTKTRWIENPLTLIPLILSNIKNFDSSASMHKFAQGEKEAFHKEQEILRRLQELPDGEQKAMETKEKIDILRHFIGYREYPKYGMINRYFIYKLALLRAGEQLVKDGILQEHEDIYFLYFEELREVVRTGQVDYELINARKRDFATFEKLTPPRILTSDGEMINGEYKRENLPKDAILGLPVSSGTVEGRARVILEMEKADLEDGDILVTAYTDPSWTPAFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATTIIKDGQQIRINGTEGYIEILD 5hv1 A 3.103 BS03 MG A 1 Q309 R311 Q309 R311 0005524,0016301,0016310,0016772 27001859 903 MKPYVLKFQEIRPHSEALVGGKGMNLGACSNIEGVHVPAGFCLTTEAYKRTLAENNEFTQLLQRLSSLKTSDMDAIREISETIRTLIQHTQIPSEIASYMDATLLDVGGYEMPFAVRSSATAEDLPHASFAGQHDTYLNIIGKDALLQHISMCWASLFTERAIIYRIQNQFDHRKVQLAVVIQQMISPEASGILFTADPITSNRKSLSIDASFGLGEALVSGLVSADSYTVRENTITNKIIATKKLAIYSLKEGGTETRILEKSQQTKQTLTDQQIIQLAKLGRKIEAYFGKPQDIEWCLAEGAFYIVQSRPITTLYPIPEVNEPGNRVYISVAHQQMMTDAMKPLGLSFYLMTTPATMYTAGGRLFVDITQSLSAKVSRDMMVNSLGQSDPLIKDALLTVINKKGFLPSIPDSSSVFELVRNSENSIKHLKQSIETKSGSDLFDFIVEDLEELKRVLFNPTSIDAIMAGMDASAWLNEHIYQWLGEKNVADKLSESAPNNITSQMGLELLDVADVIRPYPAVRAYLEQTKNPDFMNELATLEGGAETKKALEDYLQKYGMRCAGEIDLTKTRWIENPLTLIPLILSNIKNFDSSASMHKFAQGEKEAFHKEQEILRRLQELPDGEQKAMETKEKIDILRHFIGYREYPKYGMINRYFIYKLALLRAGEQLVKDGILQEHEDIYFLYFEELREVVRTGQVDYELINARKRDFATFEKLTPPRILTSDGEMINGEYKRENLPKDAILGLPVSSGTVEGRARVILEMEKADLEDGDILVTAYTDPSWTPAFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATTIIKDGQQIRINGTEGYIEILD