Structure of PDB 8bqx Chain x

Receptor sequence

>8bqxx (length=367) Species: 4932 (Saccharomyces cerevisiae)

YCSGLQCGRETSSQMKCPVCLKQGIVSIFCDTSCYENNYKAHKALHNAKD
GLEGAYDPFPKFKYSGKVKASYPLTPRRYVPEDIPKPDWAANGLPVSEQR
NDRLNNIPIYKKDQIKKIRKACMLGREVLDIAAAHVRPGITTDELDEIVH
NETIKRGAYPSPLNYYNFPKSLCTSVNEVICHGVPDKTVLKEGDIVNLDV
SLYYQGYHADLNETYYVGENISKEALNTTETSRECLKLAIKMCKPGTTFQ
ELGDHIEKHATENKCSVVRTYCGHGVGEFFHCSPNIPHYAKNRTPGVMKP
GMVFTIEPMINEGTWKDMTWPDDWTSTTQDGKLSAQFEHTLLVTEHGVEI
LTARNKKSPGGPRQRIK

3D structure

• PDB: 8bqx The dynamic architecture of Map1- and NatB-ribosome complexes coordinates the sequential modifications of nascent polypeptide chains.
• Chain: x
• Resolution: 3.8 Å

Enzymatic activity

• Enzyme Commision number: 3.4.11.18: methionyl aminopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	x	F79 P80 K81 F82 K83 Y84 K243 M338 T339 W340 P341 Q384 I386 K387	F59 P60 K61 F62 K63 Y64 K223 M318 T319 W320 P321 Q364 I366 K367

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0004177 aminopeptidase activity
• GO:0004239 initiator methionyl aminopeptidase activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0010629 negative regulation of gene expression
• GO:0016485 protein processing

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0010494 cytoplasmic stress granule
• GO:0022626 cytosolic ribosome

External links

• PDB: RCSB:8bqx, PDBe:8bqx, PDBj:8bqx
• PDBsum: 8bqx
• PubMed: 37079644
• UniProt: Q01662|MAP1_YEAST Methionine aminopeptidase 1 (Gene Name=MAP1)