Structure of PDB 7nad Chain x

Receptor sequence

>7nadx (length=539) Species: 1247190 (Saccharomyces cerevisiae BY4741) [Search protein sequence]

SLEWDNLGFSLLPWIRTGLDVMGFETMTPVQASTIPMLAGNKDVVVDSVT
GSGKTAAFVIPVLEKVVKEEANTSKFKKAHFHSLIIAPTRELSRQIESVV
LSFLEHYPSDLFPIKCQLLVGTNEATVRDDVSNFLRNRPQILIGTPGRVL
DFLQMPAVKTSACSMVVMDEADRLLDMSFIKDTEKILRLLPKQRRTGLFS
ATMRSAGSDIFKTGLRNPVRITVNSSSLKLNYCVVNPAEKLQLLVSILNN
YKFKKCIVYFPTCVSVSYFYSFIQYLGKRNILVNEVEIFSLHGKLQTSAR
TKTLTAFTDSNSVLFTTDVAARGIDIPDVDLVIQLDPPTNTDMFMHRCGR
TGRANRVGKAITFLNEGREEDFIPFMQVKNVELEELDLEVKGITANFYED
FRNWILEDRDRFDKGVKAYVAFIKYYSNHSATSIFRLQSLDYVGIAKLYG
LFRLPRMPEITKYLNWLVDPPVNMDEYKYKDKKREKERQETLKNISLIND
KKKLKSELKKKNLAWSDKTLTKERKLERKEKMSLKRKAI

3D structure

PDB

7nad Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.

Chain

Resolution

3.04 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	x	P91 R93 G124 T125 T148 G150 R151 D154 R176 F182 E187 K195 K215 P269 T270 C271 Y278 F297 S298 G301 L303 R308 K310 T311 T327 D328 V329 V430 A431 K434 Y435 R463 L464 P465 R466 M467 P468 E469 K504 K522 K523 K524 K526 N533 L534 W536 S537 D538 R545 K546 E548 R549	P88 R90 G121 T122 T145 G147 R148 D151 R173 F179 E184 K192 K212 P261 T262 C263 Y270 F289 S290 G293 L295 R300 K302 T303 T317 D318 V319 V420 A421 K424 Y425 R453 L454 P455 R456 M457 P458 E459 K483 K501 K502 K503 K505 N512 L513 W515 S516 D517 R524 K525 E527 R528

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003723	RNA binding
GO:0003724	RNA helicase activity
GO:0004386	helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016787	hydrolase activity
GO:0016887	ATP hydrolysis activity

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0000470	maturation of LSU-rRNA
GO:0006364	rRNA processing
GO:0006396	RNA processing
GO:0042254	ribosome biogenesis
GO:1902626	assembly of large subunit precursor of preribosome

	Cellular Component
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005730	nucleolus
GO:0030686	90S preribosome
GO:0030687	preribosome, large subunit precursor

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:7nad, PDBe:7nad, PDBj:7nad
PDBsum	7nad
PubMed	36482249
UniProt	P25808\|SPB4_YEAST ATP-dependent rRNA helicase SPB4 (Gene Name=SPB4)

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