Structure of PDB 8fzh Chain w

Receptor sequence

>8fzhw (length=349) Species: 562 (Escherichia coli)

KPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQLSDVSRC
FTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVDDE
RNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGG
YKEIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPE
LPDAELPDINPADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQ
DERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRNLLGSGDRSDRNR
TYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEPIIQEHQADQLAALS

3D structure

• PDB: 8fzh The ribosome termination complex remodels release factor RF3 and ejects GDP.
• Chain: w
• Resolution: 2.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	w	G118 R137 R183 G194 G301 R303 R306 Q314 R316	G110 R129 R175 G186 G293 R295 R298 Q306 R308
BS02	rna	w	G233 G234 E260 R261	G225 G226 E252 R253
BS03	rna	w	G120 T190 R195 I196 H197 T198	G112 T182 R187 I188 H189 T190
BS04	rna	w	I5 L9 L12 R15 H16 Q20 L23 R40 Y42 R228 S230 G231 A232 G233 G234 Q235 H236 V237 T239 T240 D241 R245 E256 R261 Q263 H264 R294 R295 L298	I4 L8 L11 R14 H15 Q19 L22 R39 Y41 R220 S222 G223 A224 G225 G226 Q227 H228 V229 T231 T232 D233 R237 E248 R253 Q255 H256 R286 R287 L290

Gene Ontology

Molecular Function

• GO:0016149 translation release factor activity, codon specific

Biological Process

• GO:0006412 translation
• GO:0006415 translational termination

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8fzh, PDBe:8fzh, PDBj:8fzh
• PDBsum: 8fzh
• PubMed: 39030416
• UniProt: C3TCZ2