Structure of PDB 7nad Chain w

Receptor sequence
>7nadw (length=328) Species: 1247190 (Saccharomyces cerevisiae BY4741) [Search protein sequence]
KNSKGRLDRYYARSSFKIIQINEKYGHFLLHDGAPNVGLGWVQDAFTQSQ
LTLQALKTFVTKIFRSKDYNKLIWVFQQLFEKVEATKPPASRNVSAEIFV
VCKGFKAPKRLDPRLLDPKEVFEELPDEKKVRKRQGYETEEEQIEKDLQG
LQEKQRLNVKRERRRKNEMKQKELQRMQMNMESLFNLKTAEKTGILNDLA
KGKKRMIFTMIKDKDSAADADDLESELNAMYSDYKTRRSERDAKFRAKQA
RGAITNLISKLKGQEGDHKLSSKARMIFNDPIFNNVEPFDSDYDSEEEKN
QTKKEKHSRDENLPDWFLEDAMNARPIK
3D structure
PDB7nad Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
Chainw
Resolution3.04 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.167: 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna w K39 R162 S163 T183 K184 N190 V191 A193 V240 R241 R243 R369 R372 R373 R384 Y473 R476 R480 K24 R65 S66 T86 K87 N93 V94 A96 V131 R132 R134 R161 R164 R165 R176 Y234 R237 R241
BS02 peptide w L462 E465 L466 M469 D472 R476 W698 L223 E226 L227 M230 D233 R237 W316
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0008649 rRNA methyltransferase activity
GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity
GO:0016435 rRNA (guanine) methyltransferase activity
GO:0070039 rRNA (guanosine-2'-O-)-methyltransferase activity
Biological Process
GO:0000451 rRNA 2'-O-methylation
GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0001510 RNA methylation
GO:0006364 rRNA processing
GO:0031167 rRNA methylation
GO:0032259 methylation
GO:0042254 ribosome biogenesis
Cellular Component
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0030687 preribosome, large subunit precursor

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7nad, PDBe:7nad, PDBj:7nad
PDBsum7nad
PubMed36482249
UniProtP25582|SPB1_YEAST 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase (Gene Name=SPB1)

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