Structure of PDB 8fzi Chain v

Receptor sequence

>8fziv (length=525) Species: 83333 (Escherichia coli K-12)

LSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSN
QHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPM
ELLDEVENELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQ
EVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNEFDKELFLAGEIT
PVFFGTALGNFGVDHMLDGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFK
IQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGD
RSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIR
LKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVAR
LKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLA
YIATSMVNLRLAQERYPDVQFHQTR

3D structure

• PDB: 8fzi The ribosome termination complex remodels release factor RF3 and ejects GDP.
• Chain: v
• Resolution: 3.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	v	H311 R312 H368	H309 R310 H366
BS02	rna	v	R146 D147 K177 S195	R144 D145 K175 S193

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0016149 translation release factor activity, codon specific
• GO:0016150 translation release factor activity, codon nonspecific
• GO:0019003 GDP binding
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0006412 translation
• GO:0006415 translational termination
• GO:0006449 regulation of translational termination
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8fzi, PDBe:8fzi, PDBj:8fzi
• PDBsum: 8fzi
• PubMed: 39030416
• UniProt: P0A7I4|RF3_ECOLI Peptide chain release factor RF3 (Gene Name=prfC)