Structure of PDB 6gwt Chain v

Receptor sequence

>6gwtv (length=248) Species: 562 (Escherichia coli)

DPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASE
GEHGGYKEIIAKICGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTV
AVMPELPDAELPDINPADLRIDTFRSSGAGAQHVNTTDSAIRITHLPTGI
VVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRNLLGSGDR
SDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEPIIQEHQAD

3D structure

• PDB: 6gwt Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1.
• Chain: v
• Resolution: 3.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	v	R116 R223 F227 R228 S230 G231 A232 G233 A234 Q235 H236 N238 T239 T240 D241 R245 E256 R261 Q263 H264 R295	R13 R120 F124 R125 S127 G128 A129 G130 A131 Q132 H133 N135 T136 T137 D138 R142 E153 R158 Q160 H161 R192
BS02	rna	v	D122 Q185 R195 I196 H197 T198	D19 Q82 R92 I93 H94 T95
BS03	rna	v	G118 T119 G120 R137 K160 R183 S192 Q193 G194 I196 T198 S300 G301 D302 R303 D305 Q314 R316	G15 T16 G17 R34 K57 R80 S89 Q90 G91 I93 T95 S197 G198 D199 R200 D202 Q211 R213
BS04	rna	v	H156 H236 R261 K265	H53 H133 R158 K162

Gene Ontology

Molecular Function

• GO:0003747 translation release factor activity
• GO:0016149 translation release factor activity, codon specific

Biological Process

• GO:0006415 translational termination

External links

• PDB: RCSB:6gwt, PDBe:6gwt, PDBj:6gwt
• PDBsum: 6gwt
• PubMed: 30076302
• UniProt: P0A7I0|RF1_ECOLI Peptide chain release factor RF1 (Gene Name=prfA)