Structure of PDB 5lj5 Chain v

Receptor sequence
>5lj5v (length=426) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
MLCAISGKVPRRPVLSPKSRTIFEKSLLEQYVKDTGNDPITNEPLSIEEI
VSIPNLLTSLQNEWDAIMLENFKLRSTLDSLTKKLSTVMYERDAAKLVAA
QLLMEKNEDSKDLPKSSQKNLELLQAQNYSRNIKTFPYKELNKSMYYDKW
VCMCRCEDGALHFTQLKDSKTITTITTPNPRTGGEHPAIISRGPCNRLLL
LYPGNQITILDSKTNKVLREIEVDSANEIIYMYGHNTEYFIWADNRGTIG
FQQYIVHSAKSDVEYSSGVLHKDSLLLALYSPDGILDVYNLSSPDQASSR
FPVDEEAKIKEVKFADNGYWMVVECDQTVVCFDLRKDVGTLAYPTYTIGT
VTYDIDDSGKNMIAYSNESNSLTIYKFDKKTKNWTKDEESALCLADFTDM
DVVCGDGGIAAILKTNDSFNIVALTP
3D structure
PDB5lj5 Cryo-EM structure of the spliceosome immediately after branching.
Chainv
Resolution10.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.3.2.27: RING-type E3 ubiquitin transferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna v A365 S366 A297 S298
Gene Ontology
Molecular Function
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0042802 identical protein binding
GO:0061630 ubiquitin protein ligase activity
Biological Process
GO:0000349 generation of catalytic spliceosome for first transesterification step
GO:0000398 mRNA splicing, via spliceosome
GO:0006281 DNA repair
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0016567 protein ubiquitination
GO:0070534 protein K63-linked ubiquitination
Cellular Component
GO:0000974 Prp19 complex
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0071006 U2-type catalytic step 1 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5lj5, PDBe:5lj5, PDBj:5lj5
PDBsum5lj5
PubMed27459055
UniProtP32523|PRP19_YEAST Pre-mRNA-processing factor 19 (Gene Name=PRP19)

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