Structure of PDB 8wib Chain u

Receptor sequence

>8wibu (length=85) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

ANIKSQIKRIRTNERRRLRNQSVKSSLRTAIRGFREAVDAGDKDKASELL
HATSRKLDKAASKGVIHPNQAANKKSALALALNKL

3D structure

• PDB: 8wib Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
• Chain: u
• Resolution: 3.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	u	A2 N3 K5 S6 Q7 K9 R10 I11 R12 R16 R17 R18 R20 N21 S26 S27 R29 T30 R33 H52 R56 D59 K60 S63 K64 H68 N70 Q71 A73 N74 K76 S77 A80 N84	A1 N2 K4 S5 Q6 K8 R9 I10 R11 R15 R16 R17 R19 N20 S25 S26 R28 T29 R32 H51 R55 D58 K59 S62 K63 H67 N69 Q70 A72 N73 K75 S76 A79 N83

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0070181 small ribosomal subunit rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8wib, PDBe:8wib, PDBj:8wib
• PDBsum: 8wib
• PubMed: 38245551
• UniProt: A0R102|RS20_MYCS2 Small ribosomal subunit protein bS20 (Gene Name=rpsT)