Structure of PDB 5jcs Chain s

Receptor sequence

>5jcss (length=2003) Species: 559292 (Saccharomyces cerevisiae S288C)

TFVPTHKTVSSLRQLGRKIQNSTPIMLIGKAGSGKTFLINELSKYMGCHD
SIVKIHLGEQTDAKLLIGTYTSGDKPGTFEWRAGVLATAVKEGRWVLIED
IDKAPTDVLSILLSLLEKRELTIPSRGETVKAANGFQLISTVRINEDHQK
DSSNKIYNLNMIGMRIWNVIELEEPSEEDLTHILAQKFPILTNLIPKLID
SYKNVKSIYMNTKFISLNKGAHTRVVSVRDLIKLCERLDILFKNNGINKP
DQLIQSSVYDSIFSEAADCFAGAIGEFKALEPIIQAIGESLDIASSRISL
FLTQHVPTLENLDDSIKIGRAVLLKEKLNIQKKSMNSTLFAFTNHSLRLM
EQISVCIQMTEPVLLVGETGTGKTTVVQQLAKMLAKKLTVINVSQQTETG
DLLGGYKPKTVAVPIQENFETLFNATFSLKKNEKFHKMLHRCFNKNQWKN
VVKLWNEAYKMAQSILKITNTENENENAKKKKRRLNTHEKKLLLDKWADF
NDSVKKFEAQSSSIENSFVFNFVEGSLVKTIRAGEWLLLDEVNLATADTL
ESISDLLTEPDSRSILLSEKGDAEPIKAHPDFRIFACMNPATDVGKRDLP
MGIRSRFTEIYVHSPERDITDLLSIIDKYIGKYSVSDEWVGNDIAELYLE
AKKLSDNNTIVDGSNQKPHFSIRTLTRTLLYVTDIIHIYGLRRSLYDGFC
MSFLTLLDQKSEAILKPVIEKFTLGRLKNVKSIMSYIITPFVEKNMMNLV
RATSGKRFPVLIQGPTSSGKTSMIKYLADITGHKFVRINNHEHTDLQEYL
GTYVTDDTGKLSFKEGVLVEALRKGYWIVLDELNLAPTDVLEALNRLLDD
NRELFIPETQEVVHPHPDFLLFATQNPPGIYGGRKILSRAFRNRFLELHF
DDIPQDELEIILRERCQIAPSYAKKIVEVYRQLSIERSASRLFEQKNSFA
TLRDLFRWALRDAVGYEQLAASGYMLLAERCRTPQEKVTVKKTLEKVMKV
KLDMTKGMRRLSVLVSSCLKNKEPVLLVGETGCGKTTICQLLAQFMGREL
ITLNAHQNTETGDILGAQRPVRNRSEIQYKLIKSLKTALNIANDQDVDLK
ELLQLYSKSDNKNIAEDVQLEIQKLRDSLNVLFEWSDGPLIQAMRTGNFF
LLDEISLADDSVLERLNSVLEPERSLLLAEQGSSDSLVTASENFQFFATM
NPGGDYGKKELSPALRNRFTEIWVPSMEDFNDVNMIVSSRLLEDLKDLAN
PIVKFSEWFGKKLGGGNATSGVISLRDILAWVEFINKVFPKIQNKSTALI
QGASMVFIDALGTNNTAYLAENENDLKSLRTECIIQLLKLCGDDLNLTAP
TTASNLVRVVRAMQVHKPILLEGSPGVGKTSLITALANITGNKLTRINLS
EQTDLVDLFGADAPGERSGEFLWHDAPFLRAMKKGEWVLLDEMNLASQSV
LEGLNACLDHRGEAYIPELDISFSCHPNFLVFAAQNPQYQGGGRKGLPKS
FVNRFSVVFIDMLTSDDLLLIAKHLYPSIEPDIIAKMIKLMSTLEDQVCK
RKLWGNSGSPWEFNLRDTLRWLKLLNQYSICEDVDVFDFVDIIVKQRFRT
ISDKNKAQLLIEDIFPLECNVAVYESVLKAINNNWPLVLVGPSNSGKTET
IRFLASILGPRVDVFSMNSDIDSMDILGGYEQVDLTRQISYITEELTNIV
REIISMNMKLSPNATAIMEGLNLLKYLLNNIVTPEKFQDFRNRFNRFFSH
LEGHPLLKTMSMNIEKMTEIITKEASVKFEWFDGMLVKAVEKGHWLILDN
ANLCSPSVLDRLNSLLEIDGSLLINECSQEDGQPRVLKPHPNFRLFLTMD
PKYGELSRAMRNRGVEIYIDELHSRSTAFDRLTLGFELGENIDFVSIDDG
IKKIKLNEPDMSIPLKHYVPSYLSRPCIFAQVHDILLLSDEEPIEESLAA
VIPISHLGEVGKWANNVLNCTEYSEKKIAERLYVFITFLTDMGVLEKINN
LYK

3D structure

• PDB: 5jcs Architecture of the Rix1-Rea1 checkpoint machinery during pre-60S-ribosome remodeling
• Chain: s
• Resolution: 9.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	s	K1017 V1019 K1020	K728 V730 K731

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0000055 ribosomal large subunit export from nucleus
• GO:0006364 rRNA processing
• GO:0110136 protein-RNA complex remodeling
• GO:2000200 regulation of ribosomal subunit export from nucleus

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005739 mitochondrion
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:5jcs, PDBe:5jcs, PDBj:5jcs
• PDBsum: 5jcs
• PubMed: 26619264
• UniProt: Q12019|MDN1_YEAST Midasin (Gene Name=MDN1)