Structure of PDB 7r7a Chain r

Receptor sequence

>7r7ar (length=176) Species: 1247190 (Saccharomyces cerevisiae BY4741)

PQNDYIERHIKQHGKRLDHEERKRKREARESHKISERAQKLTGWKGKQFA
KKRYAEKVSMRKKIKAHEQSKVKRMITKHTFVGEGFTRRPVKMERIIRPS
ALRQKKANVTHPELGVTVFLPILAVKKNPQSPMYTQLGVLTKGTIIEVNV
SELVWGKYAQVTNEPDRDGCVNAVLL

3D structure

• PDB: 7r7a Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
• Chain: r
• Resolution: 3.04 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	r	P2 Q3 D5 Y6 I7 E8 R23 R25 K26 A29 R30 S32 H33 S36 A39 Q40 L42 T43 G44 W45 K46 K48 Q49 R54 Y55 K58 K167 N183 T192 F194 S206 P207 T246 N247 R251 D252 N256	P1 Q2 D4 Y5 I6 E7 R22 R24 K25 A28 R29 S31 H32 S35 A38 Q39 L41 T42 G43 W44 K45 K47 Q48 R53 Y54 K57 K92 N108 T117 F119 S131 P132 T162 N163 R167 D168 N172

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding

Biological Process

• GO:0000460 maturation of 5.8S rRNA
• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000470 maturation of LSU-rRNA
• GO:0006364 rRNA processing
• GO:0042254 ribosome biogenesis
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:7r7a, PDBe:7r7a, PDBj:7r7a
• PDBsum: 7r7a
• PubMed: 36482249
• UniProt: P40078|NSA2_YEAST Ribosome biogenesis protein NSA2 (Gene Name=NSA2)