Structure of PDB 8wal Chain p

Receptor sequence

>8walp (length=1149) Species: 9823 (Sus scrofa)

EITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPI
DLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARL
RNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNG
LTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKY
AYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQ
EVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQN
VALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFL
GYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEV
RIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAG
VSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETP
EGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADAT
KIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIY
TDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYI
DTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPD
HNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSME
YLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYK
EQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDV
IIGKTVTLPENETKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRS
VRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSR
MTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGN
EVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPM
EGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIM
AIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

3D structure

• PDB: 8wal Structural Visualization of de novo Transcription Initiation.
• Chain: p
• Resolution: 8.52 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	p	P515 E516 H518 K934 K942 H1053	P500 E501 H503 K909 K917 H1028
BS02	dna	p	N188 S190 Y446 T450 Q456 K457 T746 L747 R812 G1077 R1078 R1085	N173 S175 Y431 T435 Q441 K442 T731 L732 R797 G1052 R1053 R1060
BS03	W0F	p	D792 K942 R975	D777 K917 R950
BS04	ZN	p	C1119 C1122 C1137 C1140	C1094 C1097 C1112 C1115

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005654 nucleoplasm

External links

• PDB: RCSB:8wal, PDBe:8wal, PDBj:8wal
• PDBsum: 8wal
• PubMed: 38127763
• UniProt: A0A4X1TVZ5