Structure of PDB 8phj Chain p

Receptor sequence

>8phjp (length=117) Species: 562 (Escherichia coli) [Search protein sequence]

ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRR
QRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVF
DKVAFTALVEKAKAALA

3D structure

PDB

8phj Ribosomal stalk-captured CARF-RelE ribonuclease inhibits translation following CRISPR signaling

Chain

Resolution

3.67 Å

3D
structure

Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	p	A2 R3 V4 K5 R6 G7 V8 R11 R13 H14 K15 K16 G23 Y24 Y25 A27 R28 R30 V31 R33 Q37 K41 Y45 Y47 R48 D49 R50 R51 R53 K54 R55 Q56 F57 R58 I62 N66 R70 S75 Y76 S77 N81 K84 D91 R92	A1 R2 V3 K4 R5 G6 V7 R10 R12 H13 K14 K15 G22 Y23 Y24 A26 R27 R29 V30 R32 Q36 K40 Y44 Y46 R47 D48 R49 R50 R52 K53 R54 Q55 F56 R57 I61 N65 R69 S74 Y75 S76 N80 K83 D90 R91

Gene Ontology

	Molecular Function
GO:0000900	mRNA regulatory element binding translation repressor activity
GO:0003729	mRNA binding
GO:0003735	structural constituent of ribosome
GO:0019843	rRNA binding
GO:0070180	large ribosomal subunit rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation
GO:0017148	negative regulation of translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

External links

PDB	RCSB:8phj, PDBe:8phj, PDBj:8phj
PDBsum	8phj
PubMed	38033086
UniProt	P0A7L3\|RL20_ECOLI Large ribosomal subunit protein bL20 (Gene Name=rplT)