Structure of PDB 7oqe Chain p

Receptor sequence

>7oqep (length=444) Species: 4932 (Saccharomyces cerevisiae)

ELEPFQKNFYIESETVSSMSEMEVEELRLSLDNIKIKGTGCPKPVTKWSQ
LGLSTDTMVLITKLHFGSLTPIQSQALPAIMSGRDVIGISKTGSGKTISY
LLPLLRQVKAQRPLSKHETGPMGLILAPTRELALQIHEEVTKFTEADTSI
RSVCCTGGSEMKKQITDLKRGTEIVVATPGRFIDILTLNDGKLLSTKRIT
FVVMDEADRLFDLGFEPQITQIMKTVRPDKQCVLFSATFPNKLRSFAVRV
LHSPISITINSKGMVNENVKQKFRICHSEDEKFDNLVQLIHERSEFFDAK
AIIFVSSQNICDFISKKLLNAGIVTCAIHAGKPYQERLMNLEKFKREKNS
ILLCTEVLSRGLNVPEVSLVIIYNAVKTFAQYVHTTGRTARGSRSGTAIT
LLLHDELSGAYILSKAMRDEEIKALDPLQAKELQEMSAKFESGM

3D structure

• PDB: 7oqe Structural insights into how Prp5 proofreads the pre-mRNA branch site.
• Chain: p
• Resolution: 5.9 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	p	G368 M371 K372 K379	G158 M161 K162 K169

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003724 RNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0008186 ATP-dependent activity, acting on RNA
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0000348 mRNA branch site recognition
• GO:0000398 mRNA splicing, via spliceosome
• GO:0006396 RNA processing
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing
• GO:0022613 ribonucleoprotein complex biogenesis

Cellular Component

• GO:0000243 commitment complex
• GO:0005634 nucleus

External links

• PDB: RCSB:7oqe, PDBe:7oqe, PDBj:7oqe
• PDBsum: 7oqe
• PubMed: 34349264
• UniProt: P21372|PRP5_YEAST Pre-mRNA-processing ATP-dependent RNA helicase PRP5 (Gene Name=PRP5)