Structure of PDB 8way Chain o

Receptor sequence

>8wayo (length=1452) Species: 9823 (Sus scrofa)

SACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDP
RQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCV
CFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEHGGCG
RYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFV
LGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVK
INNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRP
LKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIA
ANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKP
SDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLN
LSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVM
GIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPL
WTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGEL
IMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGH
TIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQ
TFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVI
AVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTE
FFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQ
VVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQ
EDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLR
MIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQE
NATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGE
MVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPK
TPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTV
VAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKI
NAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFL
RCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWI
LETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHV
ISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEET
VDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGME
IP

3D structure

• PDB: 8way Structural Visualization of de novo Transcription Initiation.
• Chain: o
• Resolution: 2.85 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	o	N742 R749 I779 S782 Q783 V788 G789 Q790 Q791 N792 H839 G842 E845 H1108	N707 R714 I744 S747 Q748 V753 G754 Q755 Q756 N757 H804 G807 E810 H1073
BS02	dna	o	R108 S1131 H1417	R98 S1096 H1382
BS03	dna	o	M266 P323 K331 K346 R364 Q461 A855 Y859 R1416 E1433	M231 P288 K296 K311 R329 Q426 A820 Y824 R1381 E1398
BS04	rna	o	V264 M266 F431 D495 D499	V229 M231 F396 D460 D464
BS05	ZN	o	C81 H84	C71 H74
BS06	ZN	o	C111 C114 C184	C101 C104 C149
BS07	MG	o	D495 D497 D499	D460 D462 D464
BS08	W0F	o	N493 D497	N458 D462

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006366 transcription by RNA polymerase II

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0031981 nuclear lumen
• GO:0032991 protein-containing complex

External links

• PDB: RCSB:8way, PDBe:8way, PDBj:8way
• PDBsum: 8way
• PubMed: 38127763
• UniProt: A0A8D1DPV6