Structure of PDB 8ptk Chain n

Receptor sequence

>8ptkn (length=4566) Species: 9606 (Homo sapiens)

AVQNVADVSVLQKHLRKLVPLLLEDGGEAPAALEAALEEKSALEQMRKFL
SDPQVHTVLVERSTLKEDVGDEGEEEKEFISYNINIDIHYGVKSNSLAFI
KRTPVIDADKPVSSQLRVLTLSEDSPYETLHSFISNAVAPFFKSYIRESG
KADRDGDKMAPSVEKKIAELEMGLLHLQQNIEIPEISLPIHPMITNVAKQ
CYERGEKPKVTDFGDKVEDPTFLNQLQSGVNRWIREIQKVTKLDRDPASG
TALQEISFWLNLERALYRIQEKRESPEVLLTLDILKHGKRFHATVSFDTD
TGLKQALETVNDYNPLMKDFPLNDLLSATELDKIRQALVAIFTHLRKIRN
TKYPIQRALRLVEAISRDLSSQLLKVLGTRKLMHVAYEEFEKVMVACFEV
FQTWDDEYEKLQVLLRDIVKRKREENLKMVWRINPAHRKLQARLDQMRKF
RRQHEQLRAVIVRVLRPQVFDAADANAIEEVNLAYENVKEVDGLDVSKEG
TEAWEAAMKRYDERIDRVETRITARLRDQLGTAKNANEMFRIFSRFNALF
VRPHIRGAIREYQTQLIQRVKDDIESLHDKFKVQYPQSQACKMSHVRDLP
PVSGSIIWAKQIDRQLTAYMKRVEDVLGKGWENHVEGQKLKQDGDSFRMK
LNTQEIFDDWARKVQQRNLGVSGRIFTIKLKVNFLPEIITLSKEVRNLKW
LGFRVPLAIVNKAHQANQLYPFAISLIESVRTYERTCEKVEERNTISLLV
AGLKKEVQALIAEGIALVWESYKLDPYVQRLAETVFNFQEKVDDLLIIEE
KIDLEVRSLETCMYDHKTFSEILNRVQKAVDDLNLHSYSNLPIWVNKLDM
EIERILGVRLQAGLRAWTQVLLGQAEDKAEVDMDTDAPQVSHKPGGEPKI
KNVVHELRITNQVIYLNPPIEECRYKLYQEMFAWKMVVLSLPRIQSQRYQ
VGVHYELTEEEKFYRNALTRMPDGPVALEESYSAVMGIVSEVEQYVKVWL
QYQCLWDMQAENIYNRLGEDLNKWQALLVQIRKARGTFDNAETKKEFGPV
VIDYGKVQSKVNLKYDSWHKEVLSKFGQMLGSNMTEFHSQISKSRQELEQ
HSVDTASTSDAVTFITYVQSLKRKIKQFEKQVELYRNGQRLLEKQRFQFP
PSWLYIDNIEGEWGAFNDIMRRKDSAIQQQVANLQMKIVQEDRAVESRTT
DLLTDWEKTKPVTGNLRPEEALQALTIYEGKFGRLKDDREKCAKAKEALE
LTDTGLLSGSEERVQVALEELQDLKGVWSELSKVWEQIDQMKEQPWVSVQ
PRKLRQNLDALLNQLKSFPARLRQYASYEFVQRLLKGYMKINMLVIELKS
EALKDRHWKQLMKRLHVNWVVSELTLGQIWDVDLQKNEAIVKDVLLVAQG
EMALEEFLKQIREVWNTYELDLVNYQNKCRLIRGWDDLFNKVKEHINSVS
AMKLSPYYKVFEEDALSWEDKLNRIMALFDVWIDVQRRWVYLEGIFTGSA
DIKHLLPVETQEFQSISTEFLALMKKVSKSPLVMDVLNIQGVQRSLERLA
DLLGEIQKALGEYLERERSSFPRFYFVGDEDLLEIIGNSKNVAKLQKHFK
KMFAGVSSIILNEDNSVVLGISSREGEEVMFKTPVSITEHPKINEWLTLV
EKEMRVTLAKLLAESVTEVEIFGKATSIDPNTYITWIDKYQAQLVVLSAQ
IAWSENVETALSSMGGGGDAAPLHSVLSNVEVTLNVLADSVLMEQPPLRR
RKLEHLITELVHQRDVTRSLIKSKIDNAKSFEWLSQMRFYFDPKQTDVLQ
QLSIQMANAKFNYGFEYLGVQDKLVQTPLTDRCYLTMTQALEARLGGSPF
GPAGTGKTESVKALGHQLGRFVLVFNCDETFDFQAMGRIFVGLCQVGAWG
CFDEFNRLEERMLSAVSQQVQCIQEALREHSNPNYDKTSAPITCELLNKQ
VKVSPDMAIFITMNPGSNLPDNLKKLFRSLAMTKPDRQLIAQVMLYSQGF
RTAEVLANKIVPFFKLCDEQLSSQSHYDFGLRALKSVLVSAGNVKRERIQ
KIKREKEERGEAVDEGEIAENLPEQEILIQSVCETMVPKLVAEDIPLLFS
LLSDVFPGVQYHRGEMTALREELKKVCQEMYLTYGDGEEVGGMWVEKVLQ
LYQITQINHGLMMVGPSGSGKSMAWRVLLKALERLEGVEGVAHIIDPKAI
SKDHLYGTLDPNTREWTDGLFTHVLRKIIDSVRGELQKRQWIVFDGDVDP
EWVENLNSVLDDNKLLTLPNGERLSLPPNVRIMFEVQDLKYATLATVSRC
GMVWFSEDVLSTDMIFNNFLARLRSIPLDEGEDEAQRRRKGKEDEGEEAA
SPMLQIQRDAATIMQPYFTSNGLVTKALEHAFQLEHIMDLTRLRCLGSLF
SMLHQACRNVAQYNANHPDFPMQIEQLERYIQRYLVYAILWSLSGDSRLK
MRAELGEYIRRITTVPLPTAPNIPIIDYEVSISGEWSPWQAKVPQIEVET
HKVAAPDVVVPTLDTVRHEALLYTWLAEHKPLVLCGPPGSGKTMTLFSAL
RALPDMEVVGLNFSSATTPELLLKTFDHYCEYRRTPNGVVLAPVQLGKWL
VLFCDEINLPDMDKYGTQRVISFIRQMVEHGGFYRTSDQTWVKLERIQFV
GACNPPTDPGRKPLSHRFLRHVPVVYVDYPGPASLTQIYGTFNRAMLRLI
PSLRTYAEPLTAAMVEFYTMSQERFTQDTQPHYIYSPREMTRWVRGIFEA
LRPLETLPVEGLIRIWAHEALRLFQDRLVEDEERRWTDENIDTVALKHFP
NIDREKAMSRPILYSNWLSKDYIPVDQEELRDYVKARLKVFYEEELDVPL
VLFNEVLDHVLRIDRIFRQPQGHLLLIGVSGAGKTTLSRFVAWMNGLSVY
QIKVHRKYTGEDFDEDLRTVLRRSGCKNEKIAFIMDESNVLDSGFLERMN
TLLANGEVPGLFEGDEYATLMTQCKEGAQKEGLMLDSHEELYKWFTSQVI
RNLHVVFTMNPSSEGLKDRAATSPALFNRCVLNWFGDWSTEALYQVGKEF
TSKMDLEKPNYIVPDYMPVVYDKLPQPPSHREAIVNSCVFVHQTLHQANA
RLAKRGGRTMAITPRHYLDFINHYANLFHEKRSELEEQQMHLNVGLRKIK
ETVDQVEELRRDLRIKSQELEVKNAAANDKLKKMVKDQQEAEKKKVMSQE
IQEQLHKQQEVIADKQMSVKEDLDKVEPAVIEAQNAVKSIKKQHLVEVRS
MANPPAAVKLALESICLLLGESTTDWKQIRSIIMRENFIPTIVNFSAEEI
SDAIREKMKKNYMSNPSYNYEIVNRASLACGPMVKWAIAQLNYADMLKRV
EPLRNELQKLEDDAKDNQQKANEVEQMIRDLEASIARYKEEYAVLISEAQ
AIKADLAAVEAKVNRSTALLKSLSAERERWEKTSETFKNQMSTIAGDCLL
SAAFIAYAGYFDQQMRQNLFTTWSHHLQQANIQFRTDIARTEYLSNADER
LRWQASSLPADDLCTENAIMLKRFNRYPLIIDPSGQATEFIMNEYKDRKI
TRTSFLDDAFRKNLESALRFGNPLLVQDVESYDPVLNPVLNREVRRTGGR
VLITLGDQDIDLSPSFVIFLSTRDPTVEFPPDLCSRVTFVNFTVTRSSLQ
SQCLNEVLKAERPDVDEKRSDLLKLQGEFQLRLRQLEKSLLQALNEVKGR
ILDDDTIITTLENLKREAAEVTRKVEETDIVMQEVETVSQQYLPLSTACS
SIYFTMESLKQIHFLYQYSLQFFLDIYHNVLYENPNLKGVTDHTQRLSII
TKDLFQVAFNRVARGMLHQDHITFAMLLARIKLKGTVGEPTYDAEFQHFL
RGNEIVLSAGSTPRIQGLTVEQAEAVVRLSCLPAFKDLIAKVQADEQFGI
WLDSSSPEQTVPYLWSEETPATPIGQAIHRLLLIQAFRPDRLLAMAHMFV
STNLGESFMSIMEQPLDLTHIVGTEVKPNTPVLMCSVPGYDASGHVEDLA
AEQNTQITSIAIGSAEGFNQADKAINTAVKSGRWVMLKNVHLAPGWLMQL
EKKLHSLQPHACFRLFLTMEINPKVPVNLLRAGRIFVFEPPPGVKANMLR
TFSSIPVSRICKSPNERARLYFLLAWFHAIIQERLRYAPLGWSKKYEFGE
SDLRSACDTVDTWLDDTAKGRQNISPDKIPWSALKTLMAQSIYGGRVDNE
FDQRLLNTFLERLFTTRSFDSEFKLACKVDGHKDIQMPDGIRREEFVQWV
ELLPDTQTPSWLGLPNNAERVLLTTQGVDMISKMLKMQMLEDEDAWMRTL
HTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQD
LADVVQVCEGKKKQTNYLRTLINELVKGILPRSWSHYTVPAGMTVIQWVS
DFSERIKQLQNISLAAASGGAKELKNIHVCLGGLFVPEAYITATRQYVAQ
ANSWSLEELCLEVNVTTSQGATLDACSFGVTGLKLQGATCNNNKLSLSNA
ISTALPLTQLRWVKQTNTEKKASVVTLPVYLNFTRADLIFTVDFEIATKE
DPRSFYERGVAVLCTE

3D structure

• PDB: 8ptk Molecular mechanism of dynein-dynactin complex assembly by LIS1
• Chain: n
• Resolution: 10.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	n	V1880 A1908 G1909 T1910 G1911 K1912	V1825 A1853 G1854 T1855 G1856 K1857
BS02	ATP	n	L2191 S2226 G2227 S2228 G2229 K2230 S2231 M2232 M2373 N2377	L2132 S2167 G2168 S2169 G2170 K2171 S2172 M2173 M2314 N2318
BS03	ANP	n	V2569 G2598 G2600 K2601 T2602	V2510 G2539 G2541 K2542 T2543
BS04	ANP	n	L2909 V2910 G2940 G2942 K2943	L2850 V2851 G2881 G2883 K2884

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement
• GO:0007052 mitotic spindle organization
• GO:0007097 nuclear migration
• GO:0008090 retrograde axonal transport
• GO:0031122 cytoplasmic microtubule organization
• GO:0032388 positive regulation of intracellular transport
• GO:0033962 P-body assembly
• GO:0034063 stress granule assembly
• GO:0051293 establishment of spindle localization
• GO:0051301 cell division
• GO:0060236 regulation of mitotic spindle organization
• GO:0090235 regulation of metaphase plate congression
• GO:0120162 positive regulation of cold-induced thermogenesis
• GO:1905832 positive regulation of spindle assembly

Cellular Component

• GO:0005576 extracellular region
• GO:0005737 cytoplasm
• GO:0005813 centrosome
• GO:0005829 cytosol
• GO:0005856 cytoskeleton
• GO:0005868 cytoplasmic dynein complex
• GO:0005874 microtubule
• GO:0005881 cytoplasmic microtubule
• GO:0005938 cell cortex
• GO:0016020 membrane
• GO:0030175 filopodium
• GO:0030286 dynein complex
• GO:0035578 azurophil granule lumen
• GO:0070062 extracellular exosome
• GO:1904115 axon cytoplasm

External links

• PDB: RCSB:8ptk, PDBe:8ptk, PDBj:8ptk
• PDBsum: 8ptk
• PubMed: 38547289
• UniProt: Q14204|DYHC1_HUMAN Cytoplasmic dynein 1 heavy chain 1 (Gene Name=DYNC1H1)